CAO Hong,CHEN Bai-jie,JIN Rong-rong,et al.SRAP Marker of the Unisexual Flower Gene in Pellicle Melon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(06):1-4.
与薄皮甜瓜单性花基因有关的SRAP标记研究
- Title:
- SRAP Marker of the Unisexual Flower Gene in Pellicle Melon
- 文章编号:
- 1002-2767(2010)06-0001-04
- Keywords:
- melon; unisexual flower; SRAP marker
- 分类号:
- S652
- 文献标志码:
- A
- 摘要:
- 用甜瓜单性花即雌雄同株异花的多代自交系T912作母本,两性花即雄全同株多代自交系哈甜一号作父本,配制杂交F2群体,采用相关序列扩增多态性(SRAP)分子标记结合离体分组混合分析法(BSA)筛选与甜瓜单性花有关的基因标记,在64对组合引物中,找到1个能在单性花和两性花中扩增出特异条带的标记,并在F2群体中得到验证,命名为Sme1em5,计算出其遗传距离为8.9 cM。
- Abstract:
- The objective of this study was indentify SRAP marker for monoecious characteristic on pellicle melon. BSA(Bulked Segregant Analysis)was used to search for SRAP marker linked to monoecious gene, using F2 segreganting population derived from a cross of T912(unisexual flower.) and 1-4(hermaphrodite flower). The subject found a maker sme1em5 which was related to monoecious characteristic from 64 pairs of SRAP primers.
参考文献/References:
[1]Jin W, Palmer R G,Horner H T .Molecular mapping of a male-sterile gene in soybean[J].Crop Sci.,1998,38:1681-1685. [2]Creight J D,Elmstron G W.A third muskmelon male sterility gene[J].HortScience,1984,19:268-270. [3]Kenigsbuch D,Cohen Y. The inheritance of gynoecy in muskmelon[J]. Genome,1990,33: 317-327. [4]王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998:598-602. [5]Michelmore R W,Paran I,Kesseli R V.Identification of markers linked to disease resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations[J].Natl Acad Sci.,USA,1991,88(21):9828-9832. [6]Li G,Quiros C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461. [7]Ferriol M,Pico B,Nuez F.Genetic diversity of a germplasm collection of Cucubita pepo using SRAP and AFLP markers[J].Theor Appl Genet,2003,107:271-282. [8]林忠旭,张献龙,聂以春.新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析[J].遗传学报,2004,31(6):622-626. [9]潘俊松,王刚,李效尊,等.黄瓜SRAP遗传连锁图的构建及始花节位的基因定位[J].科学通报,2005,15(2):167-172. [10]邓思立,潘俊松,何欢乐,等.黄瓜M基因连锁的SRAP分子标记[J].上海交通大学(农业科学版 ),2006,24 (3):240-244. [11]李晓慧,王从彦,张四普,等.西瓜二倍体及同源多倍体SRAP多态性分析[J].分子植物育种,2007,5 (S1),61-63. [12]Sanguinettic J,Dias N E,Simpdon A J.Rapid silver staining and recovery of PCR products separated on polyacrylamide gels [J].Biotechniques,1994,17(5):914-921. [13]方宣钧,吴为人,唐纪良.作物 DNA 标记辅助育种[M].北京:科学出版社,2001. [14]Kosambi D D.The estimation of map distances from recombination values[J].Ann Eugen,1944,12:172-175. [15]Mibus H,Tatlinglu T. Molecular characterization and isolation of the F/f gene for femaleness in cucumber (Cucumis sativusL.)[J].Theor Appl Genet,2004,109:1669-1676. [16]Noguera F J,Capel J,Alvarez J I,et al. Evelopment and mappingofa codominant SCAR marker linked to the andromonoecious gene of melon[J].Theor Appl Genet,2005,110:714-720. [17]于蓉.甜瓜单性花形状遗传规律及分子标记研究[D].杨凌:西北农林科技大学,2006. [18]张小波.甜瓜雌雄异花同株性状的遗传特征及SSR分子标记的研究[D].哈尔滨:东北农业大学,2006. [19]李征.黄瓜M基因分子标记精细定位和图位克隆初探[D].沈阳:沈阳农业大学,2007.
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备注/Memo
基金项目: 哈尔滨市科学技术计划资助项目(GJ2007GG002536)