[1]宋维富,杨雪峰,张延滨,等.东北春麦区小麦品种(系)Bx7亚基超量表达基因(Bx7OE)的分子检测[J].黑龙江农业科学,2017,(07):1-4.[doi:DOI:10.11942/j.issn1002-2767.2017.07.0001]
　SONG Wei-fu,YANG Xue-feng,ZHANG Yan-bin,et al.Detection of Overexpressed Bx7 Gene (Bx7OE) of Wheat ?Varieties(Lines) in the Northeast Spring Wheat Region by STS Markers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(07):1-4.[doi:DOI:10.11942/j.issn1002-2767.2017.07.0001]
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东北春麦区小麦品种(系)Bx7亚基超量表达基因(Bx7OE)的分子检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年07期 页码: 1-4 栏目: 出版日期: 2017-07-10
Title:
Detection of Overexpressed Bx7 Gene (Bx7OE) of Wheat ?Varieties(Lines) in the Northeast Spring Wheat Region by STS Markers
文章编号:
1002-2767（2017）07-0001-04
作者:
宋维富; 杨雪峰; 张延滨; 宋庆杰; 张春利; 辛文利; 肖志敏
黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086
Author(s):
SONG Wei-fu; YANG Xue-feng; ZHANG Yan-bin; SONG Qing-jie; ZHANG Chun-li; XIN Wen-li; XIAO Zhi-min
Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
小麦; Bx7OE基因; STS标记; 分子标记辅助选择
Keywords:
wheat;  102;  102);  font-family: Arial;  Verdana;  sans-serif;  font-size: 12px; ">Bx7 102;  102);  font-family: Arial;  Verdana;  sans-serif; ">OE?gene;  STS marker;  marker-assisted selection
分类号:
S512.1
DOI:
DOI:10.11942/j.issn1002-2767.2017.07.0001
文献标志码:
A
摘要:
高分子量谷蛋白亚基Bx7的超量表达可显著提高HMW-GS和不溶性谷蛋白大聚体的含量，使面团强度明显增强。为明确Bx7OE基因在东北春麦区小麦品种(系)中的分布情况，利用2个Bx7OE基因STS标记检测127份东北春麦区小麦品种(系)。结果表明：仅通过选择性回交转Bx7OE基因的克丰6号(7OE+8*)和龙麦20(7OE+8*)携带Bx7OE基因。
Abstract:
Due to the over-expression of the high molecular weight glutenin subunit (HMW-GS) Bx7,the content of HMW-GS and insolubility glutenin macro-polymer (GMP) were increased.And the gluten strength also was increased significantly.In order to identify the wheat varieties (lines) carrying Bx7OE gene in the northeast spring wheat region,127 wheat varieties (lines) were examined by two STS markers.The results showed that only the Kefeng 6 and Longmai20 transferred the Bx7OEgene by five consecutive backcrosses with biochemical marker assisted selection carried the Bx7OEgene.

参考文献/References:

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备注/Memo

收稿日期：2017-05-05

基金项目：国家现代农业产业技术体系建设专项资助项目(CARS-3-1-6)；国家重点研发计划资助项目(2016YFD0101802)；2013年度黑龙江省农业科学院重点基金资助项目(No.ZD011)；黑龙江省农业科学院引进博士科研启动基金资助项目(No.201507-18)
第一作者简介：宋维富(1982-)，男，黑龙江省甘南县人，博士，助理研究员，从事小麦品质遗传育种研究。E-mail：songweifu1121@126.com。

更新日期/Last Update: 2017-09-13