SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):1-3.
航天处理对小麦组织培养效果的研究
- Title:
- Study on the Effect of Space Treatment on Wheat Tissue Culture
- 文章编号:
- 1002-2767(2014)11-0001-03
- Keywords:
- wheat; space treatment; tissue culture; induce frequence; differentiation frequence; agriculture characters
- 分类号:
- S512.11
- 文献标志码:
- A
- 摘要:
- 为了促进春小麦航天诱变育种和体细胞无性系变异育种,以航天搭载的小麦纯系种子00-0387、01-0089、02-0174及其相应对照的幼穗、幼胚为外植体进行组织培养,调查了诱变频率、分化频率和S1的农艺性状。结果表明:航天处理及其相应对照的幼穗、幼胚诱导率均在98%以上,差异不显著。航天处理3份幼穗、幼胚的平均分化率均低于相应对照,而且品种间存在基因型差异。组织培养和航天加组织培养对S1农艺性状有不同程度的影响,航天加组培尤为明显。
- Abstract:
- In order to promote the spaceflight mutation breeding and somaclonal variation breeding of spring wheat,taking seed,young ear and young embryo of wheat pure lines 00-0387,01-0089 and 02-0174 as explants experienced by space treatment.Induce frequency,differentiation frequency and agriculture characters of S1 generation of space treatment and tissue culture were investigated.The results showed that the mean induce frequency of space treatment and relevant CK exceeded 98%,and there were no difference between them.The mean differentiation frequency of young ear and young embryo through space treatment was lower,and there were different gene type of material between them.Space treatment and space treatment+tissue culture had different effect on agriculture characters of S1 generation.The effect of space treatment+tissue culture was more protruding.
参考文献/References:
[1]韦彦余,赵民安,王晓军.植物体细胞无性系变异在植物性状改良中的应用[J].植物生理学通讯,2004,40(6):763-771.
[2]孙振元,韩蕾,李银凤.植物体细胞无性系变异的研究与应用[J].核农学报,2005,19(6):479-484.
[3]陈军营,何盛莲,陈新建,等.体细胞无性系变异在小麦育种中的应用[J].麦类作物学报,2005,25(2):112-115.
[4]白斌.我国小麦航天诱变育种研究进展与思考[J].甘肃农业科技,2007(4):22-24.
[5]程西永,许海霞,董中东,等.小麦航天诱变育种效果研究[J].中国农学通报,2007,23(7):598-601.
[6]刘录祥,郭会君,赵林姝,等.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592.
[7]吴伟刚,刘桂茹,杨学举.诱变与组织培养相结合在植物育种中的应用[J].中国农学通报,2005,21(11):197-201.
[8]刘进平,郑成木.诱变结合植物组织培养在植物育种中的应用[J].上海农业学报,2004,20(1):19-22.
[9]李玉全.航天搭载小麦诱变SP1代性状的研究[J].中国农学通报,2008,24(10):256-259.
[10]孙岩.提高小麦幼胚组织培养效果的初步研究[J].黑龙江农业科学,2004(1):25-27.
[11]李慧敏,赵凤梧,杨建忠,等.冬小麦轮选987航天辐射二代农艺性状变异观察及分析[J].华北农学报,2009,24(S):68-71.
[12]王美芳,杨会民,杨攀,等.冬小麦品种航天诱变后代性状分析[J].核农学报,2011,25(5):833-838.
相似文献/References:
[1]宋维富,肖志敏.小麦阶段发育理论研究与应用[J].黑龙江农业科学,2013,(02):1.
SONG Wei-fu,XIAO Zhi-min.Study and Application of Wheat Phasic Development[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):1.
[2]张宏纪,刘东军,孙 岩,等.高亲和铵转运因子在春小麦中的遗传转化研究[J].黑龙江农业科学,2014,(06):1.
ZHANG Hongji,LIU Dong-jun,SUN Yan,et al.Wheat Transformation with High affinity NH+4 Transporter Gene by Particle Bombardment Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):1.
[3]孙岩,张宏纪,辛文利,等.苗期镇压与矮壮素结合处理对小麦生育及其基部节生长的影响[J].黑龙江农业科学,2013,(02):14.
SUN Yan,ZHANG Hong-ji,XIN Wen-li,et al.Influence of Rolling and Spraying TUR at Seedling Stage on Growth Period and Growth of Base Stem for Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):14.
[4]丁琦,张延滨,李集临,等.穗发芽小麦面团流变学特性动态研究初探[J].黑龙江农业科学,2012,(05):1.
DING Qi,ZHANG Yan-bin,LI Ji-lin,et al.Preliminary Study on Dynamic Change of Dough Rheological Properties of Pre-harvest Sprouting Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):1.
[5]张欣欣,张延滨,赵海滨,等.黑龙江省小麦品种HMW-GS的RP-HPLC分析及其方法研究[J].黑龙江农业科学,2013,(05):1.
ZHANG Xin-xin,ZHANG Yan-bin,ZHAO Hai-bin,et al.Study on RP-HPLC Analysis on HMW-GS Wheat Cultivars in Heilongjiang Province and Its Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):1.
[6]徐松杰,郑根昌.不同灌水模式对小麦产量和品质的影响[J].黑龙江农业科学,2013,(05):8.
XU Song-jie,ZHENG Gen-chang.Influence of Different Irrigation Modes on the Yield and Quality of the Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):8.
[7]丁海燕,张利敏,郑茂波,等.应用小麦-黑麦代换系间杂交培育小麦新品种探讨[J].黑龙江农业科学,2011,(04):4.
DING Hai-yan,ZHANG Li-min,ZHENG Mao-bo,et al.Study on Breeding High Yield Wheat by the Hybridization of Two Different Wheat-Rye Substitution Lines[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):4.
[8]刘东军,张宏纪,郭长虹,等.小麦春化突变系T128的获得及变异分析[J].黑龙江农业科学,2013,(07):1.
LIU Dong-jun,ZHANG Hong-ji,GUO Chang-hong,et al.Obtaining and Variation Analysis of Vernalization Mutant Line T128 of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):1.
[9]姜焕焕,张延滨,李集临,等.龙麦19 Glu-D1位点近等基因系5+10供体品种醇溶蛋白块鉴定[J].黑龙江农业科学,2011,(05):1.
JIANG Huan-huan,ZHANG Yan-bin,LI Ji-lin,et al.Identification of Gliadin Block from Donor Variety ofHMW-GS 5+10 in NILs of Longmai 19 at Glu-D1 Loci[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):1.
[10]邢立伟,肖英旭.不同浓度TD植物细胞稳态剂对小麦产量的影响[J].黑龙江农业科学,2013,(09):43.
XING Li-wei,XIAO Ying-xu.Effects of Different Concentrations of TD Plant Cell Homeostasis Agent on the Yield of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):43.
备注/Memo
收稿日期:2014-07-14
基金项目:国家863计划资助项目(2012AA101202-1);黑龙江省自然科学基金重点资助项目(ZJN0704);哈尔滨市科技局科技创新人才研究专项资金资助项目(2014RFQYJ034)
第一作者简介:孙岩(1972-),女,黑龙江省哈尔滨市人,硕士,副研究员,从事小麦生物技术育种研究。E-mail:sunyan720722@sohu.com。