[1]张利国.利用原位杂交技术鉴定大麻花粉管导入材料的研究[J].黑龙江农业科学,2010,(08):14-15.
　ZHANG Li-guo.Research of Indentifying Transformed Hemp with Pollen Tube Pathway Method by GISH[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(08):14-15.
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利用原位杂交技术鉴定大麻花粉管导入材料的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2010年08 页码: 14-15 栏目: 出版日期: 2010-08-10
Title:
Research of Indentifying Transformed Hemp with Pollen Tube Pathway Method by GISH
文章编号:
1002-2767(2010)08-0014-02
作者:
张利国
黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086
Author(s):
ZHANG Li-guo
Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences,Harbin，Heilongjiang 150086
关键词:
大麻; 原位杂交; 花粉管通道
Keywords:
hemp; GISH; pollen tube pathway
分类号:
S563.3
文献标志码:
A
摘要:
应用基因组原位杂交(Genomic in situ hybridization,GISH)技术,采用花粉管通道法将亚麻黑亚14基因组导入大麻五常40并进行鉴定。结果表明：在减数分裂和有丝分裂时期通过分析所导入的7份材料的染色体状态,鉴定出成功导入的材料为1份。
Abstract:
The research indentified 7 hemp materials which was transformed with pollen tube pathway by using GISH(Genomic in situ hybridization),and analysed chromosome behavior in period of meiosis and mitosis.The experiment successfully indentified 1 hemp material by transforming,the research was very impormant for learning transformed position and transformed mechanism pollen of tube pathway method and improving transformed efficiency.

参考文献/References:

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[2] 陈春丽,郭文武,邓秀新.染色体原位杂交技术与植物体细胞杂种遗传鉴定[J].华中农业大学学报,2002,21(2):189-194.

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[4] 洪义欢,钱凯,秦秋琳,等.东乡野生稻重复序列的克隆分析及染色体定位[J].扬州大学学报(农业与生命科学版),2006,27(3):56-61.

[5] Santos A P,Wegel E,Allen G C,et al.In situ me-thods to localize transgenes and transcripts in interphase nuclei:a tool for transgenic plant research[J].Plant Methods，2006(2):18.

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备注/Memo

基金项目：国家科技攻关资助项目(2005DKA21001-19-03)；哈尔滨市科技局科技创新资助项目(2008RFXYN031)

作者简介：张利国(1978-)，男，黑龙江省哈尔滨市人，硕士，研究实习员，从事麻类分子细胞遗传学方面的研究。E-mail:zlg86@yahoo.com.cn。

更新日期/Last Update: 2014-11-06