ZHANGLi-Guo.RAPD Cluster Analysis of 27 Hemp Cultivars[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):14-16.
27 种大麻资源的RAPD 聚类分析
- Title:
- RAPD Cluster Analysis of 27 Hemp Cultivars
- 文章编号:
- 1002-2767(2009)02-0014-03
- Keywords:
- hemp; RAPD; cluster analysis
- 分类号:
- S563.3
- 文献标志码:
- A
- 摘要:
- 采用RAPD 技术对27 个大麻品种进行了分类研究。从300 个10 bp 随机引物中筛选出34 个扩增效果较好的引物进行扩增, 共产生261 条带, 其中233 条为多态性带, 占89.27%, 根据扩增结果构建反映品种间亲缘关系的UPGMA聚类图, 27 个品种可划分为3 大类。
- Abstract:
- Random amplified polymorphic DNA(RAPD)was used to study the classification of 27 hemp cultivars.Thirty-four10 bp primers selected from 300 arbitrary primers were applied fo r amplifying the hemp DNA.Total 261 bands were produced,among which 233 bands(89.27%)were polymo rphic .A dendrogram showed that genetic relationships was constructedthrough an unweighted pair-group method(UPGMA)based on the DNA polymorphism and the 27 cultivars were clusteredinto 3 main g roups.
参考文献/References:
[ 1] 宋书娟, 刘卉, 邵宏.大麻性别连锁的特异DNA 标记的初步研究[ J] .中国药物依赖性杂志, 2001 , 10(3):182-184.[ 2] Faeti V, Maodolino G , Ranalli P .Genetic diversity of Cannabi s sativagermplasm based on RAPD markers[ J] .Plant Breeding, 1996, 11(5):367-370.[ 3] 苏友波, 朱颖,林春, 等.大麻RAPD 分子标记引物筛选[ J] .中国麻业,2002 ,24(5):12-16 .[ 4] 卢江.随机放大多态性DNA[ J] .植物学报, 1993 , 35(增刊):119-127.[ 5] Williams J G K ,Kubelik A R, Livak K J , et al.DNA Polymerphismsamplified by arbimary primers are useful as genetic morkors[ J] .NucleicAcids Res., 1990(18):6531-6534.[ 6] Nei M , W H Li.Mathematical model f or studying genetical variationin terms of rest riction endonucleases[ J] .Proceedings of the NationalAcademy of Sciences of the USA ,1979 ,74:5267-5273
相似文献/References:
[1]吴广文.欧洲大麻育种及发展方向研究[J].黑龙江农业科学,2013,(06):150.
WUGuang-wen.StudyonBreedingandDevelopmentFuture ofEuropeanHemp[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):150.
[2]郑楠,宋宪友,房郁妍,等.不同收获时期对大麻全麻率的影响[J].黑龙江农业科学,2013,(08):21.
ZHENG Nan,SONG Xian-you,FANG Yu-yan,et al.Study on the Influence of Harvest Time on Total Fiber Content of Hemp[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):21.
[3]李小梅,栾 杰,张景涛,等.芫荽RAPD体系优化[J].黑龙江农业科学,2011,(06):9.
LI Xiao-mei,LUAN Jie,ZHANG Jing-tao,et al.Optimization of RAPD Reaction Conditions for?Coriander sativum [J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):9.
[4]韩微波,杜连莹,张月学,等.实践八号卫星搭载8个苜蓿品种的生理特性与RAPD分析[J].黑龙江农业科学,2011,(10):101.
HAN Wei-bo,DU Lian-ying,ZHANG Yue-xue,et al.Physiological Characteristic and RAPD Analysis of Alfalfa Carried by SJ-8 Recoverable Satellite[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):101.
[5]房郁妍.大麻栽培技术研究[J].黑龙江农业科学,2010,(06):38.
FANG Yu-yan.Research on Technology of Hemp Cultivation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):38.
[6]黄文功.亚麻种质资源的RAPD分析[J].黑龙江农业科学,2011,(08):11.
HUANG Wen-gong.RAPD Analysis of Flax Germplasm[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):11.
[7]张利国.利用原位杂交技术鉴定大麻花粉管导入材料的研究[J].黑龙江农业科学,2010,(08):14.
ZHANG Li-guo.Research of Indentifying Transformed Hemp with Pollen Tube Pathway Method by GISH[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):14.
[8]房郁妍.不同药剂对大麻性别分化的影响[J].黑龙江农业科学,2010,(10):38.
FANG Yu-yan.Effect of Different Medicament on the Hemp Sex Differentiation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):38.
[9]刘淑霞,潘冬梅,魏国江,等.四种植物提取物对大豆胞囊线虫毒杀作用[J].黑龙江农业科学,2013,(11):46.
LIU Shu-xia,PAN Dong-mei,WEI Guo-jiang,et al.The Toxic Effect of Four Plants Extracts on Soybean Cyst Nematode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):46.
[10]张利国,张效霏,房郁妍,等.大麻纤维产量构成因素的相关分析初报[J].黑龙江农业科学,2014,(01):31.
ZHANG Li-guo,ZHANG Xiao-fei,FANG Yu-yan,et al.Preliminary Study about Correlation Analysis of Yield Component Factors of Hemp Fiber[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):31.
备注/Memo
收稿日期:2008-11-31基金项目:黑龙江省农业科学院创新工程项目(2006-2008)作者简介:张利国(1978-), 男,黑龙江哈尔滨人, 硕士, 研究实习员,从事分子细胞遗传学方面的研究。Tel :13804522396 ;E-mail:zlg86@yahoo .com.cn 。