[1]于金海.玉米自交系B73全基因组cystatin抗虫基因家族分析[J].黑龙江农业科学,2010,(08):10-13.
　YU Jin-hai.Genome-Wide Analysis of cystatin Gene Family in Maize Inbred Line B73[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(08):10-13.
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玉米自交系B73全基因组cystatin抗虫基因家族分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2010年08 页码: 10-13 栏目: 出版日期: 2010-08-10
Title:
Genome-Wide Analysis of cystatin Gene Family in Maize Inbred Line B73
文章编号:
1002-2767(2010)08-0010-04
作者:
于金海
黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086
Author(s):
YU Jin-hai
Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086
关键词:
玉米; cystatin基因; 全基因组; 抗虫; 基因家族
Keywords:
maize; cystatin gene; genome wide; insect-resistance; gene family
分类号:
S513
文献标志码:
A
摘要:
半胱氨酸蛋白酶抑制剂(cystatin)基因具有cystatin结构特征的蛋白结构域，广泛存在于植物或动物体内。此类基因在植物或动物蛋白质组中具有多个功能各异的家族成员，并在抗虫中扮演重要角色。通过利用玉米B73全基因组的基因表达序列数据，对12个cystatin基因的结构组成、染色体分布、系统进化等进行了分析。鉴定了该基因家族在蛋白序列间3个高度保守基序，构建了基因家族系统进化树，为该重要基因家族的进化提供了依据的同时也为克隆和分离该类抗虫基因提供参考。
Abstract:
Cystatin genes are cysteine proteinase inhibitors with a protein pattern of cystatin-containing domain，which exist widely in many proteins from plants and animalsThis protein has many different family members distributing in plant and animal proteome，and plays key roles in plants against pestsHere，we identified complete set of 12 different cystatin gene in the maize(Zea mays L)inbred line B73 genomeThe putative cystatin genes were characterized with respect to structural diversity，chromosome distribution，phylogenetic relationship and so onThree conserved motifs were found among proteins of cystatin gene of maizePhylogenetic analysis of these proteins indicated that four groups existThe results of this study will provide evidence for evolution for cystatin gene family in maize and benefit for separating and cloning resistance gene to defend the insects

参考文献/References:

[1] Vaeck M ，Reynaerts A，Herman H，et al .Transgenic plants protected from insect attack [J]. Nature ，1987，328 ：33 - 37 

[2] Hilder V A，Gatehouse A M R，Sheerman S E，et al.A novel mechanism of insect resistance engineered into tobacoo[J].Nature，1987，33：160-163

[3] 高越峰，朱祯，肖桂芳，等.大豆Kunitz型胰蛋白酶抑制剂基因的分离及其在抗虫植物基因工程中的应用[J].植物学报，1998，40(5)：404-406

[4] Rao K V，Rathore K S，Hodges T K，et al.Expression of snowdrop lectin(GNA)in transgenic rice plants confers resistance to rice brown planthopper[J]Plant Jounal，1998，15(4)：469-477

[5] 梁辉，朱银峰，朱祯，等.雪莲花凝集素基因转化小麦及转基因小麦抗蚜性的研究[J].遗传学报，2004，31(2)：189-195

[6] Ryan C A.Proteinase inhibitor gene families，strategies for transformation improve plant defense against herb-ivores[J].Bioessays，1989，10(1):20-22

[7] Chen M S，Johnson B，Wen L，et al.Rice cystatin:bacterial expression，purification，cysteine proteinase inhibitory activity，and insect growth suppressing activity of a truncated form of the protein[J].Protein Expr Purif，1992，3(1)：41-49

[8]Takafumi Y，Hiroyuki O，Azusa S,et al.A Cysteine Protease from Maize Isolated in a Complex with Cystatin[J].Plant and Cell Physiology，2000，41(2):185-191

[9] Massonneau A，Condamine P，Wisniewski J P，et al.Maize cystatins respond to developmental cues，cold stress and drought[J]Biochim Biophys Acta，2005，1729(3):186-199

[10] Sadami O，Mayumi T，Tomokazu K，et alOryza cystatin-II，a Cystatin from Rice(Oryza sativaL.japonica)，Is a Dimeric Protein:Possible Involvement of the Interconversion between Dimer and Monomer in the Regulation of the Reactivity of Oryza cystatin-II[J].Jounal Agric Food Chem，2007，55(5):1762-1766

[11] 卢晓风，夏玉先，裴炎.植物蛋白酶抑制剂在植物抗虫与抗病中的作用[J].生物化学与生物物理学进展，1998，25(4)：328-333

[12] 柳武革，薛庆中.蛋白酶抑制剂及其在抗虫基因工程中的应用[J].生物技术通报，2000(1)：20-25

[13] 达克东，崔德才，张松，等.超强表达豇豆胰蛋白酶抑制剂基因(CpTI)转化苹果的研究[J].园艺学报，2001，28(1)：57-58

[14] Patrick S，Schnable D W,Robert S，et al.The B73 Maize Genome:Complexity，Diversity，and Dynamics[J].Science，2009，326：1112-1115

[15] Bailey T L，Gribskov MCombining evidence using P-values:application to sequences homology searches[J].Bioinformatics，1998，14:48-54

[16]杨泽峰，顾世梁，许花，等.拟南芥和水稻cystatin基因家族的生物信息学分析[J].扬州大学学报，2007，28(3)：51-57

[17] 汪结明，江海洋，赵阳，等.玉米自交系B73全基因组NBS类型抗病基因分析[J].作物学报，2009，35(3)：566-570

[18] Ledent V，Paquet O，Venvoort M.Phylogenetic analysis of the human basic helix-loop-holix proteins[J].Genome Biology，2002，3(6):30-38


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备注/Memo

作者简介：于金海(1971-)，男，黑龙江省密山市人，学士，助理研究员，从事玉米遗传育种研究。E-mail：hnygy@163.com。

更新日期/Last Update: 2014-11-06