[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(01):73-78,87.[doi:10.11942/j.issn1002-2767.2021.1.0073]
应用正交试验建立树莓组织培养快繁体系
- 文献标志码:
- A
- 摘要:
- 为促进树莓工厂化育苗，本试验采用俄罗斯引进的树莓品种“双季2381”和“单季1132”为材料，以茎段为外植体，应用正交试验对最佳腋芽萌发培养、丛生芽增殖及组培苗生根培养基进行筛选，建立树莓快繁技术体系。结果表明：腋芽萌发最佳培养基“双季2381”为6-BA 1 mg?L-1 + NAA 0.3 mg?L-1，“单季1132”为6-BA 1.0 mg?L-1 + TDZ 0.5 mg?L-1 + NAA 0.5 mg?L-1，其萌芽率分别为91.7%和92.3%，且腋芽萌发快、叶色绿。丛生芽增殖最佳培养基：“双季2381”为6-BA 2.0 mg?L-1 + TDZ 0.5 mg?L-1 + NAA 0.1 mg?L-1，“单季1132”为6-BA 1.0 mg?L-1 + TDZ 1.0 mg?L-1 + NAA 0.3 mg?L-1 ，其增殖系数分别为6.7和6.2，且丛生芽增殖快，叶色绿。组培苗生根最佳培养基“双季2381”和“单季1132”均为1/2MS + IBA 0.2 mg?L-1，其生根率分别达96.9%和95.1%，且自培苗生根快，叶色绿。
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备注/Memo
收稿日期：2020-09-27