HU Min,YUAN Lin.Extraction and Viability Determination of Soybean Nucleases[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(08):75-77.[doi:DOI:10.11942/j.issn1002-2767.2018.08.0075]
大豆核酸酶的提取及活力测定
- Title:
- Extraction and Viability Determination of Soybean Nucleases
- Keywords:
- soybean; nuclease; enzyme vitality
- 文献标志码:
- A
- 摘要:
- 为优化大豆核酸酶的提取工艺并测定其活力，分别通过考察单因素试验和正交试验对温度、提取时间、硫酸锌浓度进行优化。结果表明：以干大豆粉为原材料，温度为60 ℃、提取时间为20 h、硫酸锌浓度为0.25 mol·L-1时，大豆核酸酶活力最高，为198.24 U·mL-1。影响大豆核酸酶提取的因素主次顺序为提取时间、温度、硫酸锌浓度。
- Abstract:
- In order to optimize the extraction conditions of soybean nuclease and determine its vitality.The extracting conditions including the temperature，the extraction time，the ZnSO4?concentration were optimized by singlefactor experiment and orthogonal test.The results showed that the dry soybean powder was used,the temperature was 60 ℃,the extraction time was 20 h,the ZnSO4 concentration was 0.25 mol·L-1,and the maximum vitality of soybean nuclease was 198.24 U·mL-1.The main and secondary factors affecting the extraction of soybean nuclease were the extraction time，the temperature，the ZnSO4?concentration.
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备注/Memo
收稿日期：2018-03-23