AN Yong mei,WANG Jia jun,LI Jin rong,et al.Molecular Marker Assisted Selection on Soybean Resistant to Soybean Cyst Nematode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):15-17.
大豆抗胞囊线虫的分子标记研究
- Title:
- Molecular Marker Assisted Selection on Soybean Resistant to Soybean Cyst Nematode
- 文章编号:
- 10022767（2014）07001503
- Keywords:
- soybean; soybean cyst nematode; molecular marker
- 分类号:
- S435.651
- 文献标志码:
- A
- 摘要:
-
为了在分子水平验证大豆材料的抗病性，以对大豆胞囊线虫3号生理小种表现为抗性和感性的大豆为试材，利用已经报道的与SCN抗病基因连锁的SSR标记引物，对供试的大豆材料进行指纹图谱鉴定。结果表明：引物Satt130无扩增结果，Satt301没有多态性，Satt309和Satt082表现出多态性。聚类分析结果表明，所有供试材料被分成3大类，其中在合丰25×抗线4号正反交组合中，抗病亲本抗线4号与（抗线4号×合丰25）F2、（合丰25×抗线4号）F3抗病后代抗221、抗131聚为一类；感病亲本合丰25与感病材料（合丰25×抗线4号）F2感121被聚为一类，在这个区域将感病材料分开。而其它后代材料则聚为一类，并没有将抗感材料区分开。
- Abstract:
-
In order to verify the soybean resistance to SCN at the molecular level,taking resistance soybean and susceptible soybean to SCN as materials,and SSR markers linked to the resistance genes to SCN were used to identify the fingerprint of the selected material of soybean.The results indicated that there was no amplification result on Satt130 and no polymorphism on Satt301,but polymorphism showed on Satt309 and Satt082.The clustering analysis showed that materials were separated into 3 classes,the Hefeng 25×Kangxian 4 reciprocal cross,Kangxian 4 and resistant offspring 7,8 were in the same class,susceptible parent Hefeng 25 and susceptible material 3 were in another class,so the materials could be separated into resistance and susceptible.Other materials in the progeny were clustered in one class and did not separate the susceptible and resistant materials.
参考文献/References:
[1]Matson A L,Willams L F.Evidence of genes for resistance to the soybean cyst nematode[J].Crop Sci.,1965，22：581-590.
[2]Tautz D.Hypervariability of simple sequences as a general source of polymorphic DNA markers[J].Nucleic Acids Res.,1989,17：6463-6471.
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备注/Memo
收稿日期：2014-05-26
第一作者简介：安咏梅(1965-)，女，黑龙江省哈尔滨市人，学士，高级农艺师，从事植物保护研究。E-mail:anyongmei1965@sina.com。