GUO Yi-fan,ZHANG Hong-ji,LIU Wen-lin,et al.Study on Spring Wheat Variation of Progeny by Space Induced Mutation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):1-4.
春小麦空间诱变后代的变异研究
- Title:
- Study on Spring Wheat Variation of Progeny by Space Induced Mutation
- 文章编号:
- 1002-2767(2014)10-0001-04
- Keywords:
- wheat; space induction; character variance; microsatellite analysis
- 分类号:
- S512.0+2
- 文献标志码:
- A
- 摘要:
- 为了进一步探索和研究春小麦空间诱变后代的变异,对经返地卫星搭载纯系小麦龙麦26和克丰10号航天诱变种子种植后选育出的7份品系的农艺性状进行了调查,同时对其DNA多态性进行了分析。结果表明:变异品系较原品种在农艺性状上均有所变异,但差异不显著。变异品系与亲本之间存在着不同程度的微卫星多态性,在有效扩增的27对引物中出现3种突变类型:扩增片段增多、扩增片段减少和扩增片段长度有差异,出现的单一突变类型居多。
- Abstract:
- In order to explore and study the mutation of spring wheat offspring further,the agronomic trait and DNA polymorphism of 7 lines after the planting of space mutation seed of pure line Longmai 26 and Kefeng 10 were investigated and analyzed.The results showed that the mutant plants had more variation in yield characters than the original varieties and mostly positive mutation.Mutant plants and native species existed between different levels of microsatellite polymorphism,there were 3 types of mutations amplified among 27 groups of SSR primers:fragments amplification increased,amplified fragment decreased and different length of amplified fragment,the single mutation types were in the majority.
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备注/Memo
收稿日期:2014-06-04
基金项目:国家“863”航天育种资助项目(2012AA101202-1)
第一作者简介:郭怡璠(1981-),女,黑龙江省哈尔滨市人,硕士,助理研究员,从事春小麦辐射诱变育种及生物技术研究。E-mail:yifan-guo@163.com。