XING Gui-mei,WU Hai-hong,LI Dan,et al.Influencing Factors of Regeneration in vitro on Begonia tuberhybrida Voss cv Jinzhengri[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(12):9-12.
球根秋海棠金正日离体再生影响因素研究
- Title:
- Influencing Factors of Regeneration in vitro on Begonia tuberhybrida Voss cv Jinzhengri
- 文章编号:
- 1002-2767（2013）12-0009-04
- 分类号:
- S682.2+9
- 文献标志码:
- A
- 摘要:
- 为了建立球根秋海棠组培快繁体系，以球根秋海棠(Begonia tuberhybrida Voss)品种金正日的叶为外植体，研究了培养中各因素对叶基部的影响。结果表明：不同叶片部位外植体的诱导和分化能力不同，叶基部愈伤组织诱导和分化能力最强，诱导率和分化率分别为85.7%和85.4%；暗培养和接种前4℃低温预处理降低了外植体的诱导率和分化率，在金正日花离体培养中不宜采用；叶基部最适宜的诱导和分化培养基为MS+2,4-D 1.0 mg.-1+BA 2.0 mg.-1+NAA 0.5 mg.-1。
- Abstract:
- In order to establish rapid propagation system of tissue culture for Begonia tuberhybrida Voss，the leaves of Begonia tuberhybrida Voss cv Jinzhengri were used as explants for regeneration in vitro,influencing factors of cultivation in vitro were studied.The results showed that the rates of induction and differentiation of four kinds of explants were different,the leaf base had the highest induction and differentiation rates of 85.7% and 85.4%;the induction and differentiation rates reduced after dark culturing and in 4℃ before inoculation,which were not fit to be used in leaf base culture in vitro of Begonia tuberhybrida Voss cv Jinzhengri；the best medium for induction and differentiation of the leaf base was MS+2,4-D1.0 mg.-1+BA 2.0 mg.-1+NAA 0.5 mg.-1.
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备注/Memo
收稿日期：2013-06-29
第一作者简介：邢桂梅（1982-），女，内蒙古自治区赤峰市人，硕士，助理研究员，从事观赏植物遗传育种和组织培养研究。E-mail:xingguimei0105@163.com。