[1]黄景华,王广金,孙　岩,等.小麦花粉管通道形成时期的研究[J].黑龙江农业科学,2004,(02):20-22.[doi:10.11942/j.issn1002-2767.2004.02.0020]
　HUANG Jing-hua,WANG Guang-jin,SUN Yan,et al.Studies on the Formation Date of Pollen Tube Way of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2004,(02):20-22.[doi:10.11942/j.issn1002-2767.2004.02.0020]
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小麦花粉管通道形成时期的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2004年02 页码: 20-22 栏目: 出版日期: 2004-03-10
Title:
Studies on the Formation Date of Pollen Tube Way of Wheat
文章编号:
1002 -2767(2004)02 -0020 -03
作者:
黄景华;  王广金;  孙　岩;  刁艳玲
黑龙江省农科院作物育种所小麦辐射与生物技术研究室, 哈尔滨150086
Author(s):
HUANG Jing-hua ;  WANG Guang-jin ;  SUN Yan ;  DIAO Yan-ling
Crop Breeding Institnte of Heilong jiang Academy of Agricnltural Sciences,Harbin 150086
关键词:
小麦; 花粉管通道法; 基因枪法
Keywords:
wheat ; pallen tube way ; gene gun.
分类号:
S512 .1
DOI:
10.11942/j.issn1002-2767.2004.02.0020
文献标志码:
A
摘要:
在Leica 万能显微镜下观察了小麦花粉管的发育过程, 以及在小麦花期不同间隔时间去雄后调查结实率。研究表明, 小麦自然受粉3 min 后花粉管的最大长度相当于花粉粒的直径, 10 ～ 20min 时花粉管较长, 但尚未达到子房, 30 min 时在子房内可以观察到花粉管, 40 min 有些花粉管已接近胚囊, 且此时花粉管内胼胝质已经开始形成, 但数量较少, 60 min 后花粉管内胼胝质塞大量形成。
Abstract:
Observed the grow th course of the w heat pollen tubes on leica multiple -microscope and investigatedthe seed set ting rate in dif ferent t reatment of st igmas excised after self -pollination .The resultshow ed that the length of pollen tubes w as equal to the diameter the pollen pellet 3 min af ter pollination and the leng th w as longer in 10 ～ 20 min , but not entered into ovary .Pollen tubes could bechecked on the upper part of ovary at 30 min .several pollen tubes approached the embryo 40 min af terself -pollination and sedimentation of callus w as found inside pollen tubes , but it w as poor .There wasmuch sedimentation in the pollen tubes af ter self -pollination .The t reatment of stigmas excised af ter -pollination show ed that w heat started seed setting ?20 min after self -pollination .And the seed set ting tend to stable 40 min after self -pollinat ion .

参考文献/References:

[ 1]王广金.葡聚糖酶基因、高分子量谷蛋白优质亚基基因转化小麦的研究[ D] .哈尔滨:东北农业大学, 2002.

[ 2]周思军.大豆抗虫基因转移及其转化系统优化研究[ D] .哈尔滨:东北农业大学, 2000.
[ 3]胡适宜.被子植物胚胎学[ M] .北京:人民教育出版社, 1982.
[ 4]周光宇.植物分子育种的兴起与展望[ M] .北京:中国农业科技出版社, 1993.1-12

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备注/Memo

收稿日期:2003 -07 -10　

第一作者简介:黄景华(1976 -), 男, 黑龙江省鸡东县人, 实研, 从事小麦遗传育种研究。　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　

更新日期/Last Update: 2016-12-07