[1]宋维富,杨雪峰,刘东军,等.龙麦系列部分品种（系）Wx-B1b基因检测与淀粉特性分析[J].黑龙江农业科学,2024,(07):1-5.[doi:10.11942/j.issn1002-2767.2024.07.0001]
　SONG Weifu,YANG Xuefeng,LIU Dongjun,et al.Molecular Detection of Wx-B1b Gene and Analysis of Starch Characteristics in Some Varieties (Lines) of Longmai Series[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(07):1-5.[doi:10.11942/j.issn1002-2767.2024.07.0001]
点击复制

龙麦系列部分品种（系）Wx-B1b基因检测与淀粉特性分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2024年07 页码: 1-5 栏目: 出版日期: 2024-07-10
Title:
Molecular Detection of Wx-B1b Gene and Analysis of Starch Characteristics in Some Varieties (Lines) of Longmai Series
文章编号:
1
作者:
宋维富; 杨雪峰; 刘东军; 赵丽娟; 仇琳; 宋庆杰; 张春利; 辛文利
（黑龙江省农业科学院 作物资源研究所，黑龙江 哈尔滨 150086）
Author(s):
SONG Weifu;  YANG Xuefeng;  LIU Dongjun;  ZHAO Lijuan;  QIU Lin; SONG Qingjie;  ZHANG Chunli;  XIN Wenli
(Crop Recourse Institute,Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
关键词:
小麦; 育种; 龙麦系列; 稳定品系; Wx-B1b基因
Keywords:
wheat;  breeding;  Longmai series;  stable strain;  Wx-B1b gene
DOI:
10.11942/j.issn1002-2767.2024.07.0001
文献标志码:
A
摘要:
小麦品质改良是东北春麦区强筋小麦育种的主要目标之一，小麦籽粒淀粉特性是决定面条品质的重要因素。淀粉特性与直链淀粉含量密切相关，Wx-B1b基因为控制小麦直链淀粉含量的主效基因。为明确该基因在龙麦系列品种(系)中的分布情况，从而为面包面条兼用型强筋小麦育种和品质改良提供有用信息，利用Wx-B1b基因特异性分子标记检测了153份小麦品种(系)，并对该基因在东北春麦区强筋小麦遗传背景下淀粉特性的影响进行分析。结果表明，该特异性标记能准确鉴定Wx-B1b基因，在检测的153份材料中，82份材料含有Wx-B1b基因，占比为53.6%；黏度仪的峰值黏度在Wx-B1b和Wx-B1a基因型间差异达到显著水平（P<0.05），为淀粉特性改良的主效基因。因此在东北春麦区为进一步提高面包面条兼用型强筋小麦新品种选育效率，在亲本组配、后代选择及稳定品系处理中应加强Wx-B1b基因的应用和选择。
Abstract:
Wheat quality improvement is one of the great challenges of strong-gluten wheat breeding in the Northeast Spring Wheat Zone. The starch characteristics of wheat grains are important factors determining noodle quality. The starch characteristics are closely related to the content of amylose in wheat. The Wx-B1b gene is the major gene controlling the content of amylose in wheat. In order to clarify the distribution of this gene in the Longmai series varieties (lines) and provide useful information for quality improvement in bread and noodle type strong gluten wheat breeding, 153 wheat varieties (lines) were detected by Wx-B1b gene specific molecular markers in this study. And the influence of this gene on starch characteristics in the genetic background of strong gluten wheat in the northeast spring wheat region were analyzed. The results showed that this specific marker can accurately identify the Wx-B1b gene. Among the 153 materials detected, 82 materials contained the Wx-B1b gene, accounting for 53.6%. The peak viscosity of the viscometer showed a significant difference (P<0.05) between the Wx-B1b and Wx-B1a genotypes, indicating that it was the main gene for improving starch properties. Therefore, in the spring wheat region of Northeast China, in order to further improve the breeding efficiency of bread and noodle type strong gluten wheat, Wx-B1b gene should be used and selected in parent pairing, off spring selection, and stable strain treatment.

相似文献/References:

[1]宋维富,肖志敏.小麦阶段发育理论研究与应用[J].黑龙江农业科学,2013,(02):1.
　SONG Wei-fu,XIAO Zhi-min.Study and Application of Wheat Phasic Development[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):1.
[2]孙岩,张宏纪,王广金,等.航天处理对小麦组织培养效果的研究[J].黑龙江农业科学,2014,(11):1.
　SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):1.
[3]张宏纪,刘东军,孙 岩,等.高亲和铵转运因子在春小麦中的遗传转化研究[J].黑龙江农业科学,2014,(06):1.
　ZHANG Hongji,LIU Dong-jun,SUN Yan,et al.Wheat Transformation with High affinity NH+4 Transporter Gene by Particle Bombardment Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):1.
[4]石凤善.我国苜蓿育种研究现状及发展方向[J].黑龙江农业科学,2014,(07):155.
　SHI Feng-shan.Present Research Situation and Development Direction of Alfalfa Breeding in China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):155.
[5]孙岩,张宏纪,辛文利,等.苗期镇压与矮壮素结合处理对小麦生育及其基部节生长的影响[J].黑龙江农业科学,2013,(02):14.
　SUN Yan,ZHANG Hong-ji,XIN Wen-li,et al.Influence of Rolling and Spraying TUR at Seedling Stage on Growth Period and Growth of Base Stem for Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):14.
[6]刘凤歧,张月学,唐凤兰,等.肇东苜蓿与三种国外引进苜蓿品种间杂交的初步研究[J].黑龙江农业科学,2012,(02):93.
　LIU Feng-qi,ZAHNG Yue-xue,TANG Feng-lan,et al.Preliminary Study on Intervarietal Hybridization of Medicago sativa L.cv.Zhaodong and Three Introduced Abroad Alfalfa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):93.
[7]丁琦,张延滨,李集临,等.穗发芽小麦面团流变学特性动态研究初探[J].黑龙江农业科学,2012,(05):1.
　DING Qi,ZHANG Yan-bin,LI Ji-lin,et al.Preliminary Study on Dynamic Change of Dough Rheological Properties of Pre-harvest Sprouting Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):1.
[8]张淑华,潘国君,鄂文顺,等.黑龙江省水稻育种成就与展望[J].黑龙江农业科学,2012,(05):144.
　ZHANG Shu-hua,PAN Guo-jun,E Wen-shun,et al.Achievements and Prospects of Rice Breeding in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):144.
[9]郭震华,张淑华,刘传雪,等.水稻稻米香味基因的遗传研究及其在育种中应用的研究进展[J].黑龙江农业科学,2013,(03):143.
　GUO Zhen-hua,ZHANG Shu-hua,LIU Chuan-xue,et al.Research Progress of Genetic Studies of Rice Fragrance Gene and Its Application in Breeding[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):143.
[10]李金良.黑河地区玉米生产现状及育种对策[J].黑龙江农业科学,2012,(12):137.
　LI Jin-liang.Status and Breeding Countermeasures of Maize Production in Heihe Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):137.

备注/Memo

收稿日期：2023-11-06

基金项目：财政部和农业农村部现代农业产业技术体系春麦改良岗位科学家项目(CARS-03-12)；黑龙江省自然科学基金(YQ2020C039)；黑龙江省博士后科研启动基金(LBH-Q20176)；黑龙江省省属科研院所科研业务费(CZKYF2021-2-B007)。
第一作者：宋维富(1982-)，男，博士，副研究员，从事小麦品质遗传育种研究。E-mail：songweifu1121@126.com。通信作者：张春利(1970-)，男，博士，研究员，从事小麦遗传育种研究。E-mail:zclwheat@126.com。

更新日期/Last Update: 2024-07-10