ZENG Xiaotong,WANG Yi,CHEN Liqiang,et al.Comparison of Antioxidant Activities ofAuricularia auricularPolysaccharides from Different Producing Areas[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(01):51-57.[doi:10.11942/j.issn1002-2767.2024.01.0051]
不同产地黑木耳多糖的抗氧化活性比较
- Title:
- Comparison of Antioxidant Activities ofAuricularia auricularPolysaccharides from Different Producing Areas
- 文章编号:
- 9
- Keywords:
- Auricularia auricular; polysaccharides; antioxidant activities; TongjiangAuricularia auricular
- 文献标志码:
- A
- 摘要:
- 为促进通江黑木耳的进一步开发利用,以东北小碗耳、通江黑木耳、白背黑木耳3种不同产地的黑木耳为试材,以提取率和多糖含量为评价指标,利用热水提取法、Sevag脱蛋白得到3种黑木耳多糖,进行Molish反应分析,采用体外测试法考察不同产地黑木耳多糖的抗氧化活性。结果表明,东北小碗耳的多糖提取率和多糖含量最高,分别为2.48%和43.64%,意味着东北小碗耳的多糖提取率和多糖含量存在优势。不同产地黑木耳的多糖有不同的抗氧化活性,其中通江黑木耳多糖在抗氧化活性方面存在较为明显的优势,羟基自由基,1,1-二苯基-2-苦味基肼(DPPH·)自由基,2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+·)自由基的清除率分别高达50.11%,56.68%和32.64%,还原力能力高达0.12,表明通江黑木耳多糖抗氧化活性方面更具有潜力。
- Abstract:
- To promote the further development and utilization of TongjiangAuricularia auricular, three kinds ofA. auriculusfrom different producing areas in Northeast XiaowanAuricularia,TongjiangA. auricularand BaibeiA. auricularwere studied. With extraction rate and polysaccharide content as evaluation index,A. auricularpolysaccharides were obtained by hot water extraction and Sevag deproteinization, respectively. The different ofA. auricularpolysaccharides from different producing areas were investigated by Molish reaction. And the antioxidant activities of polysaccharides from different producing areas were investigated byin vitro. The results showed that the extraction yield and polysaccharides content of Northeast XiaowanAuriculariawere the highest, 2.48% and 43.64%, respectively, which indicated that Northeast XiaowanAuriculariahad advantages of extraction rate and polysaccharide content.A. auricularpolysaccharides from different producing areas had different antioxidant activities, and TongjiangA. auricularhad obvious advantages in antioxidant activities, the scavenging rates of hydroxyl free radical, DPPH· free radical, and ABTS+· free radical were up to 50.11%, 56.68%, 32.64%, respectively, the reducing power was as high as 0.12, which indicated that TongjiangA.uricularia auricularpolysaccharides had high antioxidant activities.
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备注/Memo
收稿日期:2023-08-11