[1]谢金燕,吴思,欧慧敏,等.平菇下脚料对番茄植株内可培养内生菌的影响[J].黑龙江农业科学,2020,(02):49-52.[doi:10.11942/j.issn1002-2767.2020.02.0049]
　XIE Jin-yan,WU Si,OU Hui-min,et al.Effects of Pleurotus ostreatus Scraps on Culturable Endophytes of Lycopersicon esculentum Plants[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(02):49-52.[doi:10.11942/j.issn1002-2767.2020.02.0049]
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平菇下脚料对番茄植株内可培养内生菌的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2020年02期 页码: 49-52 栏目: 出版日期: 2020-02-10
Title:
Effects of Pleurotus ostreatus Scraps on Culturable Endophytes of Lycopersicon esculentum Plants
作者:
谢金燕; 吴思; 欧慧敏; 范婷婷; 卢静; 李玉中
衡阳师范学院 生命科学与环境学院，湖南 衡阳 421001
Author(s):
XIE Jin-yan; WU Si; OU Hui-min; FAN Ting-ting; LU Jing; LI Yu-zhong
College of Life Sciences and Environment,Hengyang Normal University,Hengyang 421001,China
关键词:
番茄; 平菇下脚料; 可培养微生物; 内生细菌; 内生真菌
Keywords:
Lycopersicon esculentum;  Pleurotus ostreatus scraps;  culturable microbes;  endophytic bacteria;  endophytic fungi
DOI:
10.11942/j.issn1002-2767.2020.02.0049
文献标志码:
A
摘要:
为了解平菇下脚料的施用对番茄内微生物的影响，在室内盆栽条件下，以纯灭菌土为对照，在灭菌土中施入灭菌和未灭菌平菇下脚料为处理，分别移栽灭菌土培育的番茄苗，移栽30 d后，用常规分离方法分离番茄植株茎和根部的内生真菌和内生细菌，计数不同处理中可培养内生菌的数量和种类。结果表明：对照、未灭菌下脚料加土壤和灭菌下脚料加土壤的3个处理栽培的番茄中分别分离内生细菌6种、9种和7种，而分离内生真菌分别为3种、6种和4种；分离细菌总个数分别为91、86和109个，分离真菌总个数分别为5、21和30个。表明未灭活的平菇下脚料处理土壤可明显提高可培养番茄内生菌的种类，而灭活平菇下脚料处理土壤可使可培养番茄内生菌个别种类的数量明显增加。
Abstract:
In order to understand the impact of microorganism on tomato endophytes,tomato seedlings cultured in the sterilized soil were respectively planted in pure sterilized soil,sterilized soil added mushroom residues and sterilized soil added sterilized mushroom residues under the condition of indoor potted.After 30 d,endophytic fungi and endophytic bacteria of tomato plant stems and roots were separated in different treatments with conventional separation methods,and the number and species of culturable endophytes were counted.The results showed that 6 kinds,9 kinds and 7 kinds of endophytic bacteria and 3 kinds,6 kinds and 4 kinds of endophytic fungi were isolated from the control,the treatment of sterilized soil added unsterilized waste materials and the treatment of sterilized soil added sterilized waste materials respectively,and the total number of bacteria was 91,86 and 109,and the total number of fungi was 5,21 and 30 respectively.The experiment showed that the soil treated with inactivated mushroom waste could significantly improve the types of culturable endophytes in tomato plant,while the soil treated with inactivated mushroom waste could significantly increase the number of some culturable endophytes in tomato plant.

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备注/Memo

收稿日期：2019-10-27

基金项目：地方高校国家级大学生创新创业训练计划项目（教高司函〔2017〕40号：8448）；湖南省大学生研究性学习和创新性实验计划项目（湘教通〔2017〕205号：527）；衡阳师范学院大学生研究性学习和创新性实验计划项目（C1715）；湖南省自然科学基金项目（2015JJ2019）。
第一作者：谢金燕（1996-），女，在读学士，从事植物内生菌研究。E-mail: 1477952680@ qq.com。
通信作者：李玉中（1978-），男，博士，副教授，从事微生物及植物病害生物防治等研究。E-mail：yzl_2002@163.com。

更新日期/Last Update: 2020-05-06