[1]姜涛,赵琳,兰静,等.QuEChERS-超高效液相色谱串联质谱法测定玉米中的17种真菌毒素[J].黑龙江农业科学,2019,(11):106-115.[doi:10.11942/j.issn1002-2767.2019.11.0106]
　JIANG Tao,ZHAO Lin,LAN Jing,et al.Determination of 17 Mycotoxins in Maize by QuEChERS-ultra High Performance Liquid Chromatography Tandem Mass Spectrometry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(11):106-115.[doi:10.11942/j.issn1002-2767.2019.11.0106]
点击复制

QuEChERS-超高效液相色谱串联质谱法测定玉米中的17种真菌毒素

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年11期 页码: 106-115 栏目: 出版日期: 2019-11-11
Title:
Determination of 17 Mycotoxins in Maize by QuEChERS-ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
作者:
姜涛1; 赵琳2; 兰静2; 钱镭1; 王冰2; 贾雯静2; 张瑞英 2
1.黑龙江东方学院 食品与环境工程学部，黑龙江 哈尔滨 150066；2.黑龙江省农业科学院 农产品质量安全研究所/农业农村部农产品质量安全风险评估实验室，黑龙江 哈尔滨 150086
Author(s):
JIANG Tao1; ZHAO Lin2; LAN Jing2; QIAN Lei1; WANG Bing2; JIA Wen-jing2; ZHANG Rui-ying 2
1.Department of Food and Environmental，East University of Heilongjiang，Harbin 150066，China；2.Institute of Agricultural Product Quality and Safety,Heilongjiang Academy of Agricultural Sciences,Laboratory of Agricultural Product Quality and Safety Risk Assessment,Ministry of Agriculture and Rural Agriculture,Harbin 150086,China
关键词:
真菌毒素; QuEChERS; 超高效液相色谱－串联质谱; 玉米; 食品
Keywords:
mycotoxins;  QuEChERS;  ultra performance liquid chromatography-tandem mass spectrometry;  maize;  food
DOI:
10.11942/j.issn1002-2767.2019.11.0106
文献标志码:
A
摘要:
为改进我国食品安全监测的方法，采用QuEChERS净化方法结合超高效液相色谱－串联质谱（UPLC-MS/MS）对玉米中的真菌毒素进行检测，建立了玉米中共17种真菌毒素的测定方法。结果表明：17种毒素的线性相关系数（R2）均不小于0.994，检出限为0.1~20.0 μg?kg-1，加标回收率为70.76%~115.14%，相对标准偏差为2.21%~11.34%。该方法具有快速、准确、提取效率高、净化效果好、回收率高、准确灵敏等优点，此方法可适用于玉米和食品中17种真菌毒素的快速检测。
Abstract:
In order to improve the method of food safety monitoring in China,QuEChERS purification method combined with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to detect mycotoxins in maize,and a method for determination of 17 mycotoxins in maize was established.The results showed that the linear correlation coefficient (R2) of 17 toxins was not less than 0.994,the detection limit was 0.1~20.0 μg?kg-1,the recoveries were 70.76%~115.14%,and the relative standard deviation was 2.21%~11.34%.The method has the advantages of fast,accurate,high extraction efficiency,good purification effect,high recovery rate,accuracy and sensitivity,and the method can be applied to the rapid detection of 17 mycotoxins in maize and food.

参考文献/References:

［1］陈慧菲,朱天仪,陈凤香,等.QuEChERS-超高效液相色谱串联质谱法测定谷物中的8种真菌毒素[J].粮食与油脂,2016,29(5):67-70.

[2]Alshannaq A,Yu J H.Occurrence,toxicity,and analysis of major mycotoxins in food[J].International Journal of Environmental Research and Public,2017,14(6):632.
[3]Hui Y,Zhuang D,Hu X,et al.Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline[J].Analytical Methods,2018,10.DOI: 10.1039/C8AY00336J.
[4]Pallarés N,Font G,Manes J,et al.Multimycotoxin LC-MS/MS analysis in tea beverages after dispersive liquid-liquid microextraction (DLLME)[J].Journal of Agricultural and Food Chemistry,2017,65(47):10282.
[5]Man Y,Liang G,Li A,et al.Recent advances in mycotoxin determination for food monitoring via microchip[J].Toxins,2017,9(10):324.DOI：10.3390/toxins9100324.
[6]苏碧玲,谢维平,欧阳燕玲,等.QuEChERS净化－超高效液相色谱－串联质谱法测定粮食制品中4种真菌毒素[J].中国食品卫生杂志,2018,30(1):34-37.
[7]Chen Y,Chen C J,Li J,et al.Determination of 10 mycotoxin contaminants in Panax notoginseng by ultra performance liquid chromatography-tandem mass spectrometry[J].Acta Pharmaceutica Sinica,2015,50(1):81-85.
[8]Jin P G,Han Z,Cai Z X,et al.Simultaneous determination of 10 mycotoxins in grain by ultra-high-performance liquid chromatography-tandem mass spectrometry using 13C15-deoxynivalenol as internal standard[J].Food Additives & Contaminants,2010,27(12):1701-1713.
[9]Koesukwiwat U,Sanguankaew K,Leepipatpiboon N.Evaluation of a modified QuEChERS method for analysis of mycotoxins in rice[J].Food Chemistry,2014,153(9):44-51.
[10]Tamura M,Takahashi A,Uyama A,et al.A method for multiple mycotoxin analysis in wines by solid phase extraction and multifunctional cartridge purification,and Ultra-High-Performance liquid chromatography coupled to tandem mass spectrometry[J].Toxins,2012,4(12):476-486.
［11］Swayne D E,Pavade G,Hamilton K,et al.Assessment of national strategies for control of high-pathogenicity arian influerea and low-pathogenicity notifiable avian influenza in poultry with emphasis on vaccines and vaccimation［J］.Revue Scientifique et Technique(International Office of Epizootics),2011,30(3):839-870.

相似文献/References:

[1]刘畅.蔬菜中二十种磺胺类抗生素残留的QuEChERS-超高效液相色谱-串联质谱法检测[J].黑龙江农业科学,2018,(06):101.[doi:10.11942/j.issn1002-2767.2018.06.0101]
　LIU Chang.Determination of Sulfonamides in Vegetables by QuEChERS-high Performance Liquid Chromatography-tandem Mass Spectrometry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(11):101.[doi:10.11942/j.issn1002-2767.2018.06.0101]
[2]张海霞,杨静,刘畅,等.QuEChERS-超高效液相色谱-串联质谱法快速检测玉米中7种真菌毒素[J].黑龙江农业科学,2017,(07):61.[doi:10.11942/j.issn1002-2767.2017.07.0061]
　ZHANG Hai-xia,YANG Jing,LIU Chang,et al.Rapid Detection of Seven Mycotoxins in Maize by QuEChERS with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):61.[doi:10.11942/j.issn1002-2767.2017.07.0061]
[3]张海霞,徐晶,徐连伟,等.利用QuEChERS法筛查并分析哈尔滨市“地趴”储玉米中的真菌毒素[J].黑龙江农业科学,2017,(08):73.[doi:10.11942/j.issn1002-2767.2017.08.0073]
　ZHANG Hai-xia,XU Jing,XU Lian-wei,et al.Screening and Analysis of the Mycotoxin in the Maize Stored on the Ground of Harbin by QuEChERS Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):73.[doi:10.11942/j.issn1002-2767.2017.08.0073]

备注/Memo

收稿日期：2019-05-07

基金项目：国家科技重大专项子课题（2018ZX08015001-003）。
第一作者简介：姜涛(1993-)，男，硕士，从事食品质量与安全研究。E-mail： 15776898680@163.com。
通讯作者：张瑞英（1982-），女，硕士，研究员，从事农产品质量安研究。E-mail：zhruiying@163.com。

更新日期/Last Update: 2019-12-09