HAN Yang-hua.Study on Technology of Tissue Culture and Rapid ?Propagation of ?Vaccinium myrtillus? L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):12-15.[doi:10.11942/j.issn1002-2767.2016.03.0012]
野生黑果越橘组培快繁技术研究
- Title:
- Study on Technology of Tissue Culture and Rapid ?Propagation of ?Vaccinium myrtillus? L.
- 文章编号:
- 1002-2767（2016）03-0012-04
- 分类号:
- S663.9
- 文献标志码:
- A
- 摘要:
- 为了促进蓝莓种质资源的保护和开发利用，以黑果越橘茎段为材料，进行了离体培养和植株再生的研究。结果表明：茎段外植体用0.1%HgCl2消毒8~10 min为宜，最佳的丛生芽诱导培养基为改良WPM+ZT2.0 mg·L-1，丛生芽的诱导率达到55.17%，且苗健壮；最佳的增殖培养基为改良WPM+ ZT1.0 mg·L-1，增殖倍数为5.26。采用IBA 0.5 mg·L-1+NAA 0.5 mg·L-1浸泡水苔，包裹试管苗基部进行瓶外生根，生根率可达91.71%。
- Abstract:
- In order to promote the protection and development utilization of blueberry germplasm resources，taking stem section as explants,the tissue culture and plantlet regeneration of ?Vaccinium myrtillus?L.were studied.The results showed that the stem section disinfected with 0.1% for HgCl2?for 8 to 10 min,the best multiple shoot clumps induction media was the improved WPM+ZT2.0 mg· L-1,induction of the rate was 55.17%;The best proliferation medium was the improved WPM+ZT1.0 mg· L-1,proliferation ratio was 5.26.The rooting rate was up to 91.71% using sphagnum as transplant matrix when sphagna was soaked by IBA ?0.5 mg·L-1 + NAA 0.5 mg·L-1?
参考文献/References:
[1]中国科学院植物研究所．中国高等植物图鉴(第三册)[M]．北京：科学出版社，2001：212.
相似文献/References:
[1]张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014,(12):19.
ZHANG Tian-jing,LEI Jia-jun,SUN Wen-song.Study on Stem Micropropogation of Chrysanthemum cv.Jinba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):19.
[2]孟凡娟,黄凤兰,谢立波,等.扶桑(Hibiscus rosa -siensis L)的离体快繁[J].黑龙江农业科学,2007,(02):58.[doi:10.11942/j.issn1002-2767.2007.02.0058]
MENG Fan-juan,HUANG Feng-lan,XIE Li-bo,et al.Study on Rapid in Vitro Propagation of Hibiscus rosa-siensis L[J].HEILONGJIANG AGRICULTURAL SCIENCES,2007,(03):58.[doi:10.11942/j.issn1002-2767.2007.02.0058]
[3]郭艳茹,詹亚光.植物离体快繁中的常见问题及防止措施[J].黑龙江农业科学,2008,(01):19.[doi:10.11942/j.issn1002-2767.2008.01.0019]
GUO Yan-ru,ZHAN Ya-guang.Problems and Countermeasures in Plant Rapid Propagation in Vitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(03):19.[doi:10.11942/j.issn1002-2767.2008.01.0019]
[4]王海洋,龙飞,沈伟祥,等.滇黄精离体快繁体系建立及优化[J].黑龙江农业科学,2022,(05):85.[doi:10.11942/j.issn1002-2767.2022.05.0085]
WANG Hai-yang,LONG Fei,SHEN Wei-xiang,et al.Establishment and Optimization of in vitro Rapid Propagation System of Polygonatum kingianum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(03):85.[doi:10.11942/j.issn1002-2767.2022.05.0085]
备注/Memo
收稿日期：2016-01-28