GUO Xiang-feng,HOU Dian-yun,ZHANG Yu-xian,et al.Optimization Research on Extraction Process of? Polysaccharide from Smallanthus sonchifolius[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):106-110.
不同方法提取雪莲果多糖工艺的优化研究
- Title:
- Optimization Research on Extraction Process of? Polysaccharide from Smallanthus sonchifolius
- 文章编号:
- 1002-2767（2014）07-0106-05
- Keywords:
- Smallanthus sonchifolius; hot water extraction; microwave extraction; ultrasonic extraction
- 分类号:
- TQ461
- 文献标志码:
- A
- 摘要:
-
为确定雪莲果多糖提取的最佳条件，研究了热水提取、微波提取和超声波提取3种方法从雪莲果干粉中提取多糖的最佳工艺条件。结果表明：3种方法在最佳条件下雪莲果多糖得率高低顺序为：超声波法>微波法>热水法。影响微波法提取的各因素作用高低顺序为：料液比>提取温度>提取时间，提取多糖的最佳条件为料液比1∶25、温度90℃、时间35 min，多糖得率为3.24%。超声波法提取多糖的各因素顺序为：提取时间>料液比>提取温度，提取多糖的最佳条件为料液比1∶25、温度75℃、时间50 min，多糖得率为3.42%。通过紫外吸收光谱分析可知，所得粗多糖产品的纯度较高。
- Abstract:
-
In order to clear the best extraction conditions of polysaccharides from Smallanthus sonchifolius，the polysaccharide was extracted from dry powder of Smallanthus sonchifolius by hot water，microwave and ultrasonic extraction methods and the optimization conditions were studied.The results showed that under the optimum conditions three methods were ranking as the extraction ratio of polysaccharide:ultrasonic extraction＞microwave extraction＞hot water extraction.The order of affecting factors on microwave extraction was solidliquid ratio＞extraction temperature＞extraction time.The optimization conditions showed that the solidliquid ratio was 1∶25，the extraction temperature was 90℃,the time was 35 minutes and the polysaccharide extraction rate reached 3.24%.The order of affecting factors of ultrasonic extraction was extraction time＞solidliquid ratio＞extraction temperature,and the optimization conditions were showed that the solidliquid ratio was 1∶25，the extraction temperature was 75℃,the time was 50 minutes and the polysaccharide extraction rate reached 3.42%.Ultraviolet absorption spectrum showed that the polysaccharide was high purity.
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备注/Memo
收稿日期：2014-03-12
基金项目：国家自然科学基金资助项目（31372098）；河南科技大学大学生SRTP资助项目（2009131）
第一作者简介：郭香凤（1964-），女，河南省渑池县人，硕士，副教授，从事农产品贮藏加工与采后生物学研究。E-mail：gxfeng40@163.com。
通讯作者：史国安（1963-），男，河南省伊川县人，博士，教授，从事生物化学与植物资源利用研究。E-mail：gashi1963@126.com。