[1]刘炳福,宫志远,张华,等.灰树花多糖提取工艺的研究[J].黑龙江农业科学,2014,(03):104-106.
　LIU Bing fu,GONG Zhi yuan,ZHANG Hua,et al.Study on Extract Technique of Polysaccharide in Griflola frondosa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):104-106.
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灰树花多糖提取工艺的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年03 页码: 104-106 栏目: 出版日期: 2014-03-10
Title:
Study on Extract Technique of Polysaccharide in Griflola frondosa
文章编号:
1002-2767（2014）03-0104-03
作者:
刘炳福1; 宫志远2; 张华3; 姚强2
1.山东省农业科学院 科技信息研究所，山东 济南 250100；2.山东省农业科学院 农业资源与环境研究所，山东 济南 250100；3.山东中医药大学，山东 济南 250352
Author(s):
LIU Bingfu1; GONG Zhiyuan2; ZHANG Hua3; YAO Qiang2
1.Institute of Scientific Information,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100;2.Institute of Agricultural Resources and Environment,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100;3.Shandong University of Traditional Chinese medlcine,Jinan,Shandong 250352
关键词:
灰树花; 多糖; 提取工艺; 硫酸—蒽酮法
Keywords:
Griflola frondosa; polysaccharide; extract technique; anthronesulfuric acid
分类号:
S646
文献标志码:
A
摘要:
为进一步优选灰树花多糖的最佳提取工艺，研究了溶剂用量、提取时间及提取次数对灰树花多糖提取的影响。结果表明：通过提取方法的考查确定葡糖糖吸收曲线为Y=0.125 8X+0.105 5(R2=0.999 2)，浓度在0.021 4～0.128 4 mg?mL-1时与吸收度呈良好的线性关系。采用正交设计，用硫酸蒽酮法在波长618 nm处测定其吸收度，通过灰树花多糖得率的计算最终确定灰树花多糖的最佳提取工艺为水煎煮2次，煎煮时间分别为第1次提取2.0 h，第2次提取1.0 h,加28倍水，该方法简单，重现性好，提取率高。
Abstract:
In order to optimize the best extract technique for Polysaccharide of Griflola frondosa,the effect of dosage of solvent,extraction time and times on extracting of Griflola frondosa were studied.The results showed that through the determine of extraction method of polysaccharide,the amylose sorption degree linearity relation was Y=0.1258X+0.105 5(R2=0.999 2)，the range of concentration was in 0.021 4～0.128 4 mg?mL-1，and had good linearity relation with absorbance.Orthogonal experiment was adopt,the absorbance was measured by anthronesulfuric acid at 618 nm.The best technique for Griflola frondosa polysaccharide was boiling 2 times,decoction time was 2.0 h first and then 1.0 h,amount of added water were 28 times.The simple method which was good for extracting of polysaccharide had good reproducibility and high extraction efficiency.

参考文献/References:

[1]杨海，耿传信，周学锋.灰树花多糖药理研究综述[J].中国执业药师，2012,9（3）：30-33. [2]冯怡，韩宁，徐德生.麦冬多糖含量测定方法的研究[J].中成药，2006,28（5）:705-707. [3]马昌豪，刘红艳，彭慧敏，等.不同品种玫瑰花多糖含量测定[J].食品与药品，2011,13（11）：432-433. [4]朱伟.蒽酮硫酸比色法测定香菇多糖含量[J].北方药学，2011，8(8):8-10.

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备注/Memo

收稿日期：2013-12-18基金项目：国家食用菌产业技术体系资助项目（CARS-24）第一作者简介：刘炳福（1964-），男，山东省青岛市人，农艺师，从事农业技术与推广研究。E-mail:qbswjs2008@sina.com。通讯作者：张华（1967-），女，山东省青岛市人，副教授，硕士生导师，从事中药新药研究。E-mail：zhongyiyao77@126.com。

更新日期/Last Update: 2014-09-16