WANG Ruo-ding,WANG Chao,MENG Fan-juan,et al.The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):106-109.
工布乌头中三种生物碱HPLC法测定优化
- Title:
- The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L.
- 文章编号:
- 1002-2767（2014）01-0106-04
- Keywords:
- Aconitum kongboense L.; high performance liquid chromatography(HPLC); mesaconitine; Talatisamine; aconitine
- 分类号:
- R284.2
- 文献标志码:
- A
- 摘要:
- 为优化工布乌头中生物碱的测定方法，采用HPLC法测定工布乌头中新乌头碱、塔拉萨敏和乌头碱的含量。结果表明:采用10%氨水1 mL、乙醚20 mL，浸泡24 h后超声处理30 min可有效地对工布乌头中生物碱进行定量分析。
- Abstract:
- In order to optimize the extracting method of alkaloids in Aconitum kongboense L.,the content of mesaconitine,talatisamine and aconitine were determined by HPLC.The results showed that 10% ammonia 1 mL,ether 20 mL,soak for 24 h,30 min after ultrasonic treatment could be used to the quantitative analysis of the alkaloids of Aconitum kongboense L..
参考文献/References:
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备注/Memo
基金项目：国家“十二五”科技支撑计划资助项目(2011BAI13B06)