[1]边道林,孙磊,王爽,等.嗜冷产甲烷菌群的选育及产甲烷效能研究[J].黑龙江农业科学,2014,(10):44-48.
　BIAN Dao-lin,SUN Lei,WANG Shuang,et al.Study on Cultivation and Performance of Psychrophilic Methane-Producing Bacteria[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):44-48.
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嗜冷产甲烷菌群的选育及产甲烷效能研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年10 页码: 44-48 栏目: 出版日期: 2014-10-10
Title:
Study on Cultivation and Performance of Psychrophilic Methane-Producing Bacteria
文章编号:
1002-2767（2014）10-0044-05
作者:
边道林1; 孙磊2; 王爽2; 郭炜3
1.黑龙江省农业科学院，黑龙江 哈尔滨 150086；
2.黑龙江省农业科学院 土壤肥料与环境资源研究所，黑龙江 哈尔滨 150086；
3.黑龙江省农业科学院 农村能源研究所，黑龙江 哈尔滨 150086
Author(s):
BIAN Dao-lin1; SUN Lei2; WANG Shuang2; GUO Wei3
1.Heilongjiang Academy of Agriculture Sciences,Harbin,Heilongjiang 150086;
2.Institute of Soil Fertilizer and Environment Resources of Heilongjiang Academy of Agriculture Sciences,Harbin,Heilongjiang 150086;
3.Rural Energy Researchof Institute of Heilongjiang Academy of Agriculture Sciences,Harbin,Heilongjiang 150086
关键词:
低温; 产甲烷菌; 克隆文库; 沼气
Keywords:
low temperature; methanogen; clone library; biogas
分类号:
Q93;TK6
文献标志码:
A
摘要:
筛选低温环境的复合产甲烷菌群更适合提高系统低温产甲烷效能，从自然低温厌氧环境采集活性污泥，利用人工培养基进行富集驯化，以温度为胁迫因子研究复合菌群的产甲烷效能。同时，运用克隆文库的分子生物学技术对复合低温产甲烷菌群的细菌和产甲烷古菌进行了解析。结果表明:采集的自然生境的低温复合菌群细菌克隆文库共获得80条有效序列，分布在11个细菌门，最优势细菌属Proteobacteria门（72.5%），其次是Bacteroidetes门（8.8%）。产甲烷菌克隆文库获得20条有效序列，可鉴定的产甲烷古菌为Methanosaetaceae科和Methanosarcinaceae科。经富集驯化的低温复合菌群在18℃时产气量最大，沼气产量为0.25 m3.(m3reactor d)-1，甲烷浓度为56.3%。
Abstract:
Screening of the composite methane-producing bacteria in low temperature is more suitable to improve the efficiency of methane-producing.Collecting of activated sludge from natural low temperature anaerobic and enrichment domestication were conducted by artificial medium,methane-producing performance was studied taking temperature as stress factor.In addition,the molecular biological technique of clone library was used to analyze the structure of bacteria and archaea.The results showed that 80 sequences were obtained from bacterial clone libraries,and distributed in 11 bacterial phyla.The most dominant bacteria was Proteobacteria(72.5%)followed by Bacteroidetes(8.8%).There were 20 sequences obtained from Methanogenic clone library.The classified archaea were Methanosaetaceae and Methanosarcinaceae.The maximum biogas yield of psychrophilic methane-producing bacteria after cultivation achieved 0.25 m3. (m3reactor d)-1,and methane concentration achieved 56.3% while the fermentation temperature was 18℃.

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备注/Memo

收稿日期：2014-08-05
基金项目： “十二五”科技支撑计划资助项目（2013BAD07B01）；黑龙江省科技特派员农村创新创业资助项目（GC12B711）;黑龙江省博士后基金资助项目
第一作者简介：边道林(1978-)，男，山东省郓城县人，硕士，助理研究员，从事科研管理和农业资源环境研究。E-mail:330834043qq.com。
通讯作者：王爽（1981-），博士，副研究员，从事环境和微生物等方面研究。E-mail：wangshuang0726@163.com。

更新日期/Last Update: 2014-12-05