LIU Dong-jun,ZHANG Hong-ji,GUO Chang-hong,et al.Obtaining and Variation Analysis of Vernalization Mutant Line T128 of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):1-4.
小麦春化突变系T128的获得及变异分析
- Title:
- Obtaining and Variation Analysis of Vernalization Mutant Line T128 of Wheat
- 文章编号:
- 1002-2767（2013）07-0001-04
- Keywords:
- wheat; vernalization mutantion line; vernalization gene; variation of non-target agronomic traits
- 分类号:
- S512
- 文献标志码:
- A
- 摘要:
- 春小麦龙辐10号外源基因转化后代中发现了变异植株，并选育出弱冬习性的突变系T128。为了明确T128的突变机理，利用特异标记检测了龙辐10号和突变系T128的春化基因。结果表明：龙辐10号和T128的春化基因分别是Vrn-A1a、vrn-B1、vrn-D1、vrn-B3和vrn-A1、Vrn-B1、vrn-D1、vrn-B3。龙辐10号具有显性基因Vrn-A1a，而突变系T128的显性基因为Vrn-B1，致使突变系T128变为弱冬习性，生长发育时期延迟，表明外源基因转化过程可以导致基因变异。
- Abstract:
- Agronomic trait variation was found in the offsprings of genetic transformation in wheat Longfu 10,and mutant line T128 with medium winterness was bred.In order to determine mutation mechanism,vernalization genes of Longfu 10 and mutant line T128 were tested by designed mark.The result showed that vernalization genes type of Longfu10 were Vrn-A1a,vrn-B1,vrn-D1 and vrn-B3,that of mutant line T128 were vrn-A1,Vrn-B1,vrn-D1 and vrn-B3.Longfu 10 had dominant gene Vrn-A1a，dominant gene of mutant line T128 was Vrn-B1.It induced medium winterness of mutant line T128,delayed developmental stage.The research considered that genetic transformation induces variation of non-target agronomic traits.
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备注/Memo
收稿日期：2013-04-02
基金项目：国家863课题资助项目（2012AA1012-1）
第一作者简介：刘东军（1978-），男，陕西省富平县人，硕士，助理研究员，从事小麦辐射与生物技术研究。E-mail：dongdong415@126.com。
通讯作者：张宏纪（1969-），男，黑龙江省抚远县人，博士，研究员，从事小麦辐射与生物技术研究。E-mail：fumai@163.com。