ZHAO Xiao-feng,YU Wen-qing,TIAN Yan-hong,et al.Isolating and Screening of NH3 Removing Strains andBacterial Agent Preparation of Chicken Manure[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):85-87.
鸡粪除氨菌株的分离、筛选与菌剂配制
- Title:
- Isolating and Screening of NH3 Removing Strains andBacterial Agent Preparation of Chicken Manure
- 文章编号:
- 1002-2767（2012）06-0085-03
- Keywords:
- microbial deodorization; chicken manure; ammonia removing strains; strains complex; bacterial agent preparation
- 分类号:
- S141.4
- 文献标志码:
- A
- 摘要:
- 为减少鸡粪高温堆肥氨气的释放量，于2010~2011年，采用嗅味法进行初筛，采用硼酸吸收，稀硫酸滴定的方法进行复筛研究。结果表明：初筛得到具有除臭能力的菌株449株，并最终分离了细菌KB217、放线菌KA46和真菌KF21三个菌株，使鸡粪堆肥的氨气释放量分别降低了59.40%、55.05%和56.38%。初步鉴定细菌KB217为微球菌属，放线菌KA46为链霉菌属，真菌KF21为青霉属。拮抗试验证明3株菌之间无拮抗作用，菌株复合试验结果表明3株菌的混合制剂能使鸡粪堆肥的氨气释放量降低67.33%，并且缩短了氨气的释放时间。
- Abstract:
- Aiming at reducing the release amount of NH3 of chicken manure during high temperature compost，primary screened by smell and secondary screening by the method of boric acid absorption，then titrated with dilute sulfuric acid were studied from 2010 to 2011.The result showed that primary screened 449 strains，and finally obtained three strains that could remove NH3，named KB217，KA46 and KF21，their removal rate were 59.40%、55.05% and 56.38%.Preliminary identified KB217 was micrococuss，KF21was penicillium，and KA46 was streptomyces.There was no antagonism between three strains，the result of strains complex experiment indicated that the agent mixed with three stains could decrease the release amount of NH3 by 67.33%，and shorten its release time.
参考文献/References:
[1]陈瑞勤.鸡粪除臭微生物的筛选[J].中国饲料添加剂，2009（6）：24-26. [2]东秀珠，蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社，2011. [3]布坎南R E，吉本斯N E.伯杰细菌鉴定手册[M].8版.北京：科学出版社，1984. [4]阎逊初.放线菌的分类和鉴定[M].北京:科学出版社，1974. [5]魏景超.真菌鉴定手册[M].上海:科学技术出版社，1979.
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备注/Memo
基金项目： 黑龙江省科技厅科技攻关资助项目（GA09B502-1）