[1]付洪冰,刘丽艳,刘昭军,等.白藜芦醇合酶基因表达载体构建及对马铃薯的遗传转化[J].黑龙江农业科学,2009,(05):1-4.[doi:10.11942/j.isn1002-2767.2009.05.0001]
　FU Hong-bing,LIU Li-yan,LIU Zhao-jun,et al.Construction of Resveratrol Synthase Gene Expression Vector and theGenetic Transformation of Potato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(05):1-4.[doi:10.11942/j.isn1002-2767.2009.05.0001]
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白藜芦醇合酶基因表达载体构建及对马铃薯的遗传转化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年05 页码: 1-4 栏目: 出版日期: 2009-09-10
Title:
Construction of Resveratrol Synthase Gene Expression Vector and theGenetic Transformation of Potato
文章编号:
1002-2767(2009)05-0001-04
作者:
付洪冰 1 ; 刘丽艳 2 ; 刘昭军 2 ; 盛万民 2 ; 王　 珣 2 ; 李　 铁2; 王俊河 2 ; 杨　 学 2 ; 王秀君 2 ; 赵　 曦2
(1.东北农业大学园艺学院,黑龙江哈尔滨 150030;2.黑龙江省农业科学院,黑龙江哈尔滨 150086)
Author(s):
FU Hong-bing1; LIU Li-yan2;  LIU Zhao-jun 2 ;  SHENG Wan-min2;  WANG Xun 2 ;  LI Tie2;  WANG Jun-he 2 ; YANGXue 2 ; WANG Xiu-jun 2 ; ZHAO Xi 2
(1.Horticultural College of Northeast Agricultural University, Harbin, Heilongjiang 150030;2 Hei-longjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)
关键词:
白藜芦醇合酶; 马铃薯; 表达载体; 农杆菌介导; 遗传转化
Keywords:
resveratrol synthase; potato; expression vector; agrobacterium-mediated; genetic transformation
分类号:
S531
DOI:
10.11942/j.isn1002-2767.2009.05.0001
文献标志码:
A
摘要:
利用从葡萄中克隆到的白藜芦醇合酶基因(Resveratrol Synthase, 简称 RS)构建了含有 35S 组成型启动子的植物表达载体 P35s-2300-RS, 应用农杆菌介导方法对马铃薯品种进行遗传转化。通过对抗性芽、生根、再生植株的筛选, 得到5 株再生小苗。 经 PCR、Southern检测证实, 有3株为真正的转基因植株。
Abstract:
The resveratrol synthase gene expression vector has been constructedwith the construction of constitutive 35S pro-moter of the plant expression vector P35s-2300-RS(Resveratrol Synthase, referred to asRS), The gene was originally clonedfrom grapes.The gene was transferred by the Agrobacterium-mediatedinto the potato varieties.The study obtained5 regen-erated seedlings by screening the resistant shoot, root, the regenerated plant, 3 of which were the real transgenic plant con-firmed by PCR,Southern Testing .

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备注/Memo

基金项目:黑龙江省留学回国人员科技活动项目(黑人函(2005)307);黑龙江省博士后资助项目(LSZH-04086)第一作者简介:付洪冰(1979-), 男, 黑龙江人, 博士, 从事蔬菜分子育种研究。通讯作者:刘丽艳, 博士, 研究员。 E-mail:liuliyanlocus @yahoo.com.cn。

更新日期/Last Update: 2016-11-27