[1]叶永亮,杜　波.黄瓜再生体系和遗传转化的建立[J].黑龙江农业科学,2009,(05):5-7.[doi:10.11942/j.isn1002-2767.2009.05.0005]
　YE Yong-liang,DU Bo.Construction of Cucumber Regeneration System and GeneticTransformation System[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(05):5-7.[doi:10.11942/j.isn1002-2767.2009.05.0005]
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黄瓜再生体系和遗传转化的建立

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年05 页码: 5-7 栏目: 出版日期: 2009-09-10
Title:
Construction of Cucumber Regeneration System and GeneticTransformation System
文章编号:
1002-2767(2009)05-0005-02
作者:
叶永亮; 杜　波
(哈尔滨市农业科学院,黑龙江哈尔滨 150070)
Author(s):
YE Yong-liang; DU Bo
(Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang 150070)
关键词:
黄瓜; 子叶节; 不定芽; 再生体系; 遗传转化体系; PGRs
Keywords:
cucumber; cotyledon node; adventitious shoot; regeneration system; genetic transformation system; PGRs
分类号:
S532
DOI:
10.11942/j.isn1002-2767.2009.05.0005
文献标志码:
A
摘要:
以黄瓜(Cucumissativus L.)哈研3 号为试验材料, 采用组织培养技术系统探讨以子叶或子叶节为外植体时不定芽的诱导效率, 最终确定以子叶节作为再生体系及遗传转化体系的试验材料;并系统探讨了不同 PGRs 浓度对不定芽诱导、不定芽伸长及生根阶段的影响;建立了哈研 3号黄瓜的高频再生体系;并在子叶节根端膨大阶段进行了抗生素(Km)浓度的摸索, 建立了较为适宜的遗传转化体系, 为转基因工作打下坚实的基础。
Abstract:
Taking cucumber(Cucumissativus L. )“ Hayan No.3” as the test material, using tissue culture technology systemto explore inductionefficiency of adventitious shoot for cotyledon or cotyledon node as explants.Then, the cotyledon node asa regeneration system and genetic transformation system was determined as test materials.And systems of different PGRsconcentration on adventitious bud induction, adventitious shoot elongation and rooting stages of impact were discussed.Final-ly, the“Hayan No.3” the high-frequency regeneration system of cucumber was set up.Cotyledon node at the root end-stageexpansion of the antibiotic (Km )concentration was explored, and a more appropriate genetic transformation system wasconstructed for genetically modified job thus laying a solid foundation.

参考文献/References:

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备注/Memo

第一作者简介:叶永亮(1981-), 男, 河北省南皮县人, 学士, 中级农艺师,从事黄瓜分子育种研究, E-mail:yyl8998@126.com

更新日期/Last Update: 2016-11-27