MENG Fan-juan,HUANG Feng-lan,XIE Li-bo,et al.Study on Rapid in Vitro Propagation of Hibiscus rosa-siensis L[J].HEILONGJIANG AGRICULTURAL SCIENCES,2007,(02):58-60.[doi:10.11942/j.issn1002-2767.2007.02.0058]
扶桑(Hibiscus rosa -siensis L)的离体快繁
- Title:
- Study on Rapid in Vitro Propagation of Hibiscus rosa-siensis L
- 文章编号:
- 1002 -2767(2007)02-0058-03
- Keywords:
- Hibiscus rosa -siensis L ; in vitro culture
- 分类号:
- S685 .220.43
- 文献标志码:
- A
- 摘要:
- 以扶桑带嫩芽的茎段为外植体进行快繁。试验结果表明:最佳基本培养基为1/ 2MS ;为防止褐化可加入抗坏血酸(100 mg/ L);最有效诱导丛生芽的激素配比为6 -BA(4.0 mg/ L)+NAA(0 .5 mg/ L);最佳诱导生根的激素配比为1/ 2MS +100 mg/ L 抗坏血酸+NAA1 .0 mg/ L 。该技术为扶桑的规模化生产提供了技术保障。
- Abstract:
- The tissue culture o f tende r stem from Hibiscus rosa -siensis L w as done .The results showed :the suitable basic medium was 1/2MS , V -tamin C w as used to prevent becoming brown , the proper bud inductio n hormone pro po r tion was 6-BA(4.0 mg/ L)+NAA(0 .5 mg/ L), the suitable ro oting ho rmo ne propo rtio n was1/ 2MS +Vc100 mg/ L +NAA1.0 mg/ L, A macropro pag atio n method to the large scale pr oductio n o f Hibiscus rosa -siensis L plants w as provided .
参考文献/References:
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[ 4] 袁翠陵.扶桑[ J] .植物杂志, 2003 ,(5):26 .
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[ 6] 黄凤兰, 梁冰, 卢宝伟,等.小叶绿萝的组织培养技术研究[ J] . 北方园艺, 2004 ,(6):76-77 .
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备注/Memo
收稿日期:2007 -01 -08
第一作者简介:孟凡娟(1975 -), 女, 黑龙江省兰西县人, 博士, 讲师, 从事植物遗传研究。E -mai l:mf j19751 @163 .com 。