[1]刘文林,张宏纪,孙岩,等.平阳霉素对小麦雌雄配子诱变效果的研究[J].黑龙江农业科学,2021,(01):1-5.[doi:10.11942/j.issn1002-2767.2021.1.0001]
　Xiang-yu,JPZHANG Bao-hui.Effects of Pingyangmycin on Mutagenesis of Male and Female Gametes in Triticum aestivum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(01):1-5.[doi:10.11942/j.issn1002-2767.2021.1.0001]
点击复制

平阳霉素对小麦雌雄配子诱变效果的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年01 页码: 1-5 栏目: 出版日期: 2021-01-10
Title:
Effects of Pingyangmycin on Mutagenesis of Male and Female Gametes in Triticum aestivum L.
作者:
刘文林1; 张宏纪1; 孙岩1; 唐婧泉1; 杨淑萍1; 王翔宇1; 张宝辉2
1.黑龙江省农业科学院 作物资源研究所，黑龙江 哈尔滨 150086；2.内蒙古呼伦贝尔市特泥河农牧场，内蒙古 呼伦贝尔 021000
Author(s):
Xiang-yu1; 〖JP〗ZHANG Bao-hui2
1.Crop Resources Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China；2.Tenihe Farm of Hulunbuir of Inner Mongolia Autonomous Region,Hulunbuir 021000,China
关键词:
小麦; 化学诱变; 平阳霉素; 性状变异
Keywords:
wheat(Triticum aestivum L.);  chemical mutagenesis;  pingyangmycin;  character variation
DOI:
10.11942/j.issn1002-2767.2021.1.0001
文献标志码:
A
摘要:
为提高化学诱变剂的诱变效果，本研究采用叶鞘注入和颖壳注入的处理方法将浓度分别为20，40和80 μg?mL-1 的平阳霉素注入2个小麦品种的叶鞘和颖壳内，使其直接作用于小麦雌雄配子,调查平阳霉素诱变小麦后代的变异情况。结果表明：活体诱变处理的龙辐06K508和龙06-6798的M1出苗率都随着平阳霉素浓度的增大而降低。小穗颖壳注入法在两个品种间半致死剂量存在差异，而叶鞘注入法没有达到半致死剂量。两种处理方式在M1、M2的株高、生育期和芒型等性状上均发生了变异。经数据分析显示，叶鞘注入法M1代株高、穗长、分蘖数、小穗数和主穗粒重与CK相比变化不显著，而小穗颖壳注入法株高、穗长和小穗数与CK相比变化显著，叶鞘注入法M2代穗长小穗数与CK相比变化显著，而小穗颖壳注入法株高和小穗数与CK相比变化达到显著和极显著水平。说明小颖壳注入法直接作用于雌雄配子，保湿效果好，药效时间长，在诱变后代中能产生丰富的变异，可以在诱变育种中加以利用。
Abstract:
In order to improve the mutagenic effects of chemical mutagens,pingyangmycin (20,40 and 80 μg?mL-1) were injected into the glume and sheath of two wheat varieties and made a direct effect on wheat gametes by husk and sheath injection.Agronomic traits of wheat genetic offspring was investigated.The results showed that in vivo mutagenesis treatment,the emergence rate of the M1 of both Longfu 06K508 and Longfu 06-6798 decreased with the increase of concentration.There was a difference in the lethal dose between the two varied by glume injection,but the median lethal dose was not achieved by leaf sheath injection.The two treatments showed variation in plant height,growth period and awn type of M1 and M2.Data analysis showed that the plant height,ear length,and spikelet number of M1 generation was significantly different to CK by glume injection method.However,the method of leaf sheath injection was not significant.Compared with CK,the ear length and spikelet number of the M2 by leaf sheath injection method changed significantly,while the plant height and spikelet number of the glume injection method changed significantly and extremely significantly compared with CK.It indicated that the glume injection method has direct effect on male and female gametes,it was a good moisturizer and the effect time was long.It could produce abundant variations in mutagenesis progeny and be used in mutation breeding.

参考文献/References:

［1］张月学.诱变在作物育种中的应用[J].黑龙江农业,1994(3):43-44.

[2]林赴田，李电东，杨小平,等.平阳霉素的抗肿瘤作用与临床前药理研究[J].中华肿瘤杂志,1979,1(3):161-166.
[3]赵丽梅,许耀奎.平阳霉素Pingyangmycin对大麦的诱变效应[J].核农学报,1990,4(4):199-205.
[4]孙德全.穗茎注射平阳霉素对小麦诱变效果的研究.核农学报,1999,13(6):321-324.
[5]夏英武,吴关庭,舒庆尧,等.平阳霉素对水稻诱变效应的研究[J].核农学报,1997,11(1):26-30.
[6]朴铁夫,原亚萍,李全国,等.平阳霉素（pingyangmycin）对小麦诱变效应的研究[J].核农学报,1998,12(3):139-145.
[7]王红,陈发棣,赵宏波,等.平阳霉素对盆栽小菊意大利红的诱变效应[J].南京农业大学学报,2007,6(1):7-8.
[8]王红.平阳霉素对离体培养条件下小菊品种诱变效应研究[D].南京:南京农业大学,2006.
[9]原亚平,许耀奎.平阳霉素（pingyangmycin）对大豆诱变效应的研究[J].作物学报,1993,19(1):7-16.〖ZK)〗
[10]黄丽华,赖宇文,陈庭俊.平阳霉素对烟草诱变作用的初探[J].作物杂志,1998,8(4):7-9.
[11]张秋英,叶定生,金美玉,等.平阳霉素诱导小麦农艺性状变异的研究[J].麦类作物学报,2002,8(4):15-17.
[12]赵明霞,隋炯明,王晓杰,等.平阳霉素对花生体细胞胚胎发生的影响[J].核农学报,2011,25(2):242- 246.
[13]吴关婷,夏英武,舒庆尧,等.平阳霉素对不同水稻品种的生物学效应[J].浙江农业大学学报,1997,23(1):1-6.
[14]王晶珊,姜亚男,尹秀波,等.离体诱变定向培育高油花生新品种宇花9号[J].生物工程学报,2019,5(7):1277-1285.
[15]陈洋,高兰英,邵艳军,等.EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析[J].核农学报,2011,25(4):617-621.
[16]姚恒,白戈,谢贺,等.EMS对烤烟K326种子发芽率及幼苗成活率的处理效应[J].分子植物育种,2019(14):261-267.
[17]乔利仙,隋炯明,赵丽兰,等.平阳霉素离体诱变花生M2农艺性状分析[J].核农学报,2014,28(6):955-960.

相似文献/References:

[1]宋维富,肖志敏.小麦阶段发育理论研究与应用[J].黑龙江农业科学,2013,(02):1.
　SONG Wei-fu,XIAO Zhi-min.Study and Application of Wheat Phasic Development[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):1.
[2]孙岩,张宏纪,王广金,等.航天处理对小麦组织培养效果的研究[J].黑龙江农业科学,2014,(11):1.
　SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):1.
[3]张宏纪,刘东军,孙 岩,等.高亲和铵转运因子在春小麦中的遗传转化研究[J].黑龙江农业科学,2014,(06):1.
　ZHANG Hongji,LIU Dong-jun,SUN Yan,et al.Wheat Transformation with High affinity NH+4 Transporter Gene by Particle Bombardment Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):1.
[4]孙岩,张宏纪,辛文利,等.苗期镇压与矮壮素结合处理对小麦生育及其基部节生长的影响[J].黑龙江农业科学,2013,(02):14.
　SUN Yan,ZHANG Hong-ji,XIN Wen-li,et al.Influence of Rolling and Spraying TUR at Seedling Stage on Growth Period and Growth of Base Stem for Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):14.
[5]丁琦,张延滨,李集临,等.穗发芽小麦面团流变学特性动态研究初探[J].黑龙江农业科学,2012,(05):1.
　DING Qi,ZHANG Yan-bin,LI Ji-lin,et al.Preliminary Study on Dynamic Change of Dough Rheological Properties of Pre-harvest Sprouting Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(01):1.
[6]张欣欣,张延滨,赵海滨,等.黑龙江省小麦品种HMW-GS的RP-HPLC分析及其方法研究[J].黑龙江农业科学,2013,(05):1.
　ZHANG Xin-xin,ZHANG Yan-bin,ZHAO Hai-bin,et al.Study on RP-HPLC Analysis on HMW-GS Wheat Cultivars in Heilongjiang Province and Its Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):1.
[7]徐松杰,郑根昌.不同灌水模式对小麦产量和品质的影响[J].黑龙江农业科学,2013,(05):8.
　XU Song-jie,ZHENG Gen-chang.Influence of Different Irrigation Modes on the Yield and Quality of the Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):8.
[8]丁海燕,张利敏,郑茂波,等.应用小麦-黑麦代换系间杂交培育小麦新品种探讨[J].黑龙江农业科学,2011,(04):4.
　DING Hai-yan,ZHANG Li-min,ZHENG Mao-bo,et al.Study on Breeding High Yield Wheat by the Hybridization of Two Different Wheat-Rye Substitution Lines[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(01):4.
[9]刘东军,张宏纪,郭长虹,等.小麦春化突变系T128的获得及变异分析[J].黑龙江农业科学,2013,(07):1.
　LIU Dong-jun,ZHANG Hong-ji,GUO Chang-hong,et al.Obtaining and Variation Analysis of Vernalization Mutant Line T128 of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):1.
[10]姜焕焕,张延滨,李集临,等.龙麦19 Glu-D1位点近等基因系5+10供体品种醇溶蛋白块鉴定[J].黑龙江农业科学,2011,(05):1.
　JIANG Huan-huan,ZHANG Yan-bin,LI Ji-lin,et al.Identification of Gliadin Block from Donor Variety ofHMW-GS 5+10 in NILs of Longmai 19 at Glu-D1 Loci[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(01):1.

备注/Memo

收稿日期：2020-11-02

基金项目：黑龙江省农业科学院“农业科技创新跨越工程”专项（HNK2019CX04-06）；国家重点研发计划（2016YFD0102101）。
第一作者：刘文林（1976-），男，硕士，助理研究员，从事小麦生物技术和遗传育种研究。E-mail：2533383054@qq.com。
通信作者：张宏纪（1969-），男，博士，研究员，从事小麦遗传育种研究。E-mail：fumai@163.com。

更新日期/Last Update: 2021-02-23