LIU Wenlin,ZHANG Hongji,SUN Yan,et al.Detection and Composition Analysis of Powdery Mildew Resistance Genes Introduced from Russian Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(01):1-6.[doi:10.11942/j.issn1002-2767.2024.01.0001]
俄罗斯春小麦抗白粉病基因检测及其组成分析
- Title:
- Detection and Composition Analysis of Powdery Mildew Resistance Genes Introduced from Russian Spring Wheat
- 文章编号:
- 1
- Keywords:
- wheat; powdery mildew; resistance gene; molecular marker
- 文献标志码:
- A
- 摘要:
- 为了解俄罗斯春小麦抗白粉病基因的组成及分布规律，利用已开发的Pm2、Pm3b、Pm4、Pm8、Pm13和Pm21标记对外引俄罗斯251份春小麦品种进行了检测和分析。结果表明，在251份小麦品种中，分布5种抗白粉病基因，其中Pm2和Pm3分布频率较高，均为90.44%；Pm13为57.77%，抗病基因Pm4分布频率为10.36%，Pm8基因的分布频率为5.58%，抗病基因Pm21在引入的俄罗斯春小麦中没有分布，有6份材料没有检测出含有上述基因标记。俄罗斯251份春小麦品种中小麦抗白粉病基因组合共有15种类型，其中116份小麦品种以Pm2/Pm3/Pm13类型所占比例为46.22%；其他14种类型所占比例依次为，Pm2/Pm3类型比例为23.90%；Pm2/Pm3/Pm4类型比例为4.78%；Pm2/Pm3/Pm4/Pm13类型比例为4.38%；Pm2类型比例为3.58%；Pm3和Pm2/Pm13类型比例均为2.79%；Pm3/Pm13和Pm2/Pm3/Pm8类型比例均为2.39%；Pm2/Pm3/Pm4/Pm8和Pm2/Pm3/Pm8/Pm13类型比例均为1.20%；Pm13类型比例为0.79%；Pm2/Pm8、Pm3/Pm4/Pm13和Pm3/Pm8/Pm13比例为0.40%；本研究明确了俄罗斯春小麦品种抗白粉病基因的组成和分布频率，可进一步利用外引材料开展抗白粉病育种。
- Abstract:
- In order to understand the composition and distribution of powdery mildew resistance genes in Russian spring wheat， 251 spring wheat cultivars introduced from Russia were detected and analyzed by using the markersPm2， Pm3b， Pm4， Pm8， Pm13andPm21. The results showed that 5 powdery mildew resistance genes are distributed in 251 wheat varieties， and the distribution frequencies ofPm2andPm3were 90.44%. The second gene wasPm13(57.77%)， then the disease resistance genePm4(10.36%)， and the lowest gene wasPm8(5.58%). The disease resistance genePm21was not distributed in Russian spring wheat， and 6 materials were not detected to contain the above gene markers. There were 15 powdery mildew resistance gene combinations among 251 spring wheat varieties. Among 116 wheat varieties，Pm2/Pm3/Pm13had the highest proportion (46.22%). The proportions of the other 14 types were as follows:Pm2/Pm3type accounted for 23.90%;Pm2/Pm3/Pm4accounted for 4.78%; The proportion ofPm2/Pm3/Pm4/Pm13was 4.38%;Pm2type accounted for 3.58%;Pm3andPm2/Pm13accounted for 2.79%.Pm3/Pm13 and Pm2/Pm3/Pm8accounted for 2.39%. The proportion ofPm2/Pm3/Pm4/Pm8 and Pm2/Pm3/Pm8/Pm13was 1.20%.Pm13types accounted for 0.8%; The proportions ofPm2/Pm8， Pm3/Pm4/Pm13andPm3/Pm8/Pm13were all 040%. This study clarified the composition and distribution frequency of powdery mildew resistance genes in Russian spring wheat， providing theoretical guidance for wheat powdery mildew resistance breeding.
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备注/Memo
收稿日期：2023-09-06