[1]王海洋,龙飞,沈伟祥,等.滇黄精离体快繁体系建立及优化[J].黑龙江农业科学,2022,(05):85-90.[doi:10.11942/j.issn1002-2767.2022.05.0085]
　WANG Hai-yang,LONG Fei,SHEN Wei-xiang,et al.Establishment and Optimization of in vitro Rapid Propagation System of Polygonatum kingianum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(05):85-90.[doi:10.11942/j.issn1002-2767.2022.05.0085]
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滇黄精离体快繁体系建立及优化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年05 页码: 85-90 栏目: 出版日期: 2022-05-10
Title:
Establishment and Optimization of in vitro Rapid Propagation System of Polygonatum kingianum
作者:
王海洋1; 2; 龙飞2; 沈伟祥1; 2; 董章宏1; 2; 王正德1; 2; 夏茂甜1; 2; 赵文植1; 2; 辛培尧1; 2
1.西南林业大学 园林园艺学院/国家林业和草原局西南风景园林工程技术研究中心，云南 昆明 650224；2.西南林业大学 林学院/西南地区生物多样性保育国家林业和草原局重点实验室，云南 昆明 650224
Author(s):
WANG Hai-yang1; 2; LONG Fei2; SHEN Wei-xiang1; 2; DONG Zhang-hong1; 2; WANG Zheng-de1; 2; XIA Mao-tian1; 2; ZHAO Wen-zhi1; 2; XIN Pei-yao1; 2
1.College of Landscape and Horticulture，Southwest Forestry University/Southwest Research Center for Landscape Architecture Engineering,State Forestry and Grassland Administration,Kunming 650224，China; 2.College of Forestry，Southwest Forestry University/Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Kunming 650224,China
关键词:
滇黄精; 离体快繁; 基质筛选
Keywords:
In order to promote the industrial production of Polygonatum kingianum;  using young rhizome of Polygonatum kingianum as explant; the variety concentration ratio and medium type of exogenous growth regulator suitable for axillary bud induction; multiplication and rooting of P.kingianum were studied; and transplanting experiments were performed.The results showed that the best medium for rhizome induction and differentiation of P.kingianum was 1/2MS+6-BA 2.0 mg·L-1+2; 4-D 0.2 mg·L-1+sucrose 30 g·L-1+agar 5.5 g·L-1; The propagation medium of stem segments with buds of P.kingianum was formulated as MS+6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1+sucrose 30 g·L-1+agar 5.5 g·L-1; The best rooting medium formulation was 1/2MS+NAA 0.2 mg·L-1+sucrose 30 g·L-1+agar 5.5 g·L-1;  The optimum substrate ratio for the growth of P.kingianum plantlets was humus soil∶red sandstone soil∶perlite=1∶1∶1; graftings had 100% survival rate.
DOI:
10.11942/j.issn1002-2767.2022.05.0085
文献标志码:
A
摘要:
为促进滇黄精的工厂化生产，选取滇黄精（Polygonatum kingianum Coil.et Hemsl）当年生幼嫩根状茎作为外植体，研究适合滇黄精腋芽诱导、增殖、生根培养的外源生长调节剂的种类、浓度配比、培养基类型，并进行移栽试验。结果表明：滇黄精根状茎诱导分化的最适培养基配方为1/2MS+6-BA 2.0 mg·L-1+2,4-D 0.2 mg·L-1+蔗糖30 g·L-1+琼脂5.5 g·L-1；滇黄精带芽茎段增殖最适培养基配方为MS+6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1+蔗糖30 g·L-1+琼脂5.5 g·L-1；滇黄精带芽茎段生根最适培养基配方为1/2MS+NAA 0.2 mg·L-1+蔗糖30 g·L-1+琼脂5.5 g·L-1；滇黄精组培苗生长最适基质配比是腐殖质土∶红泥土∶珍珠岩为1∶1∶1，其成活率可达100%。
Abstract:
Polygonatum kingianum; rapid propagation in vitro; matrix optimization

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备注/Memo

收稿日期：2022-01-15

基金项目：云南省科技人才与平台计划项目（202205AF150022）；云南省科技厅科技计划重点研发项目（2018BB005）。 

第一作者：王海洋（1995-），男，硕士研究生，从事林木遗传育种学习与工作。E-mail：458083617@qq.com。

通信作者：辛培尧(1975-)，男，博士，教授，从事植物遗传育种与快繁研究。E-mail:xpytgyx@163.com。

更新日期/Last Update: 2022-07-15