[1]牛庭莉,张树权,韩喜财,等.大麻雌化处理方法的研究进展[J].黑龙江农业科学,2022,(01):100-103.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0100]
　NIU Ting-li,ZHANG Shu-quan,HAN Xi-cai,et al.Research Progress on Female Treatment of Cannabis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(01):100-103.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0100]
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大麻雌化处理方法的研究进展

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年01 页码: 100-103 栏目: 出版日期: 2022-01-10
Title:
Research Progress on Female Treatment of Cannabis
作者:
牛庭莉1; 张树权2; 韩喜财3; 牛江帅1; 吴 荣1; 程欣然1; 戴凌燕1
1.黑龙江八一农垦大学 生命科学技术学院，黑龙江 大庆 163319；2.黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086；3.黑龙江省科学院 大庆分院，黑龙江 大庆 163319
Author(s):
NIU Ting-li1; ZHANG Shu-quan2; HAN Xi-cai3; NIU Jiang-shuai1; WU Rong1; CHENG Xin-ran1; DAI Ling-yan1
1.College of Life Science and Technology，Heilongjiang Bayi Agricultural University，Daqing 163319，China; 2.Institute of Idustrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086，China； 3.Daqing Branch,Heilongjiang Academy of Sciences，Daqing 163319，China
关键词:
大麻; 雌化; 全雌种子; 大麻素; 处理方法
Keywords:
Cannabis;  feminization;  all-female seeds;  cannabinoid;  treatment method
DOI:
DOI:10.11942/j.issn1002-2767.2022.01.0100
文献标志码:
A
摘要:
为提高大麻育种效率，本文对诱导大麻雌株形成的物理化学方法、环境因素等及全雌种子产生的方法进行概述，并提出未来利用现代分子生物学技术对大麻进行雌化的可能途径。一方面是建立大麻植株性别早期鉴选技术，从而提高栽培效率并缩短育种进程；另一方面可通过分子设计育种来达到雌化目标，即建立稳定高效的大麻遗传转化体系和克隆大麻调控雌性分化的重要功能基因。
Abstract:
In order to improve the efficiency of hemp breeding, this paper summarized the physicochemical methods and environmental factors of inducing the formation of female plants of hemp and the methods of producing all female seeds, and put forward the possible ways of using modern molecular biology technology to feminize hemp in the future. On the one hand, it is the early sex discrimination technology of hemp plants, so as to improve the cultivation efficiency and shorten the breeding process; On the other hand, we can achieve the goal of feminization through molecular design breeding, that is, to establish a stable and efficient genetic transformation system of hemp and clone the important functional genes of hemp regulating female differentiation.

参考文献/References:

参考文献： 

［1］张建春,关华,刘雪强,等.汉麻种植与初加工技术[M].北京：化学工业出版社,2009.

[2］李仕金,辛培尧,周军,等.大麻性别研究进展[J].中国麻业科学,2008(2):110-113.

[3］陈其本.大麻利用的新趋势[J].中国麻作,1999(1):49-50.

[4］赵铭森,康红梅.山西省发展大麻生产的现状及构想[J].安徽农学通报,2007,13(10):140.

[5］尹燕霞,吴和珍,魏群.火麻仁的研究进展[J].中国中医药信息杂志,2003,10(6):92- 94.

[6］吴姗.外源激素对大麻中大麻素含量的影响及转录组分析[D].北京：中国农业科学院,2020.

[7］SOLDATOVA N A,KHRYANIN V N.The effects of heavy metal salts on the phytohormonal status and sex expression in marijuana[J].Russian Journal of Plant Physiology,2010,57(1):96-100.

[8］YIN T J,QUINN J A.Tests of a mechanistic model of one hormone regulating both sexes in Buchloe dactyloides(Poaceae)[J].American Journal of Botany,1995,82(6):745-751. [9］YIN T J,QUINN J A.Tests of a mechanistic model of One hormone regulating both sexes in Cucumis sativus(Cucurbitaceae)[J].American Journal of Botany,1995,82(12):1537-1546. 

[10］BOISSAY E,DELAIGUE M,SALLAUD C,ESNAULT R,KAHLEM G.Predominant expression of a peroxidase gene in staminate flowers of Mercurialis annua[J].Physiologia Plantarum,1996,96(2):251-257.

[11］CHAILAKHYAN M K,KHRYANIN V N.The role of rootsin sex expression in hemp plants[J].Planta,1978,138(2):185-187.

[12］CHAILAKHYAN M K,KHRYANIN V N.The role of leavesin sex expression in hemp and spinach[J].Planta,1979,144(2):205-207.

[13］CHAILAKHYAN M K.Gormonal′naya teoriya razvitiya rastenii.Hormonal theory of plant development[M].Moscow:AkadNaukSSSR,1937.

[14］HERICH R.Gibberellin and sex differentiation of flowering plants［J］.Nature(Lond.),1960,188:599-600. 

[15］房郁妍.不同药剂对大麻性别分化的影响[J].黑龙江农业科学,2010(10):38-39.

[16］RAM H Y M,JAISWAL V S.Induction of male flowers of female plants of Cannabis sativa by gibberellin and its inhibition by abscisic acid[J].Planta,1972,105(3):263-266. [17］RAM H Y M,JAISWAL V S.Induction of female flowers on male plants of Cannabis sativa L.by 2-chloroethanephos-phonic acid[J].Experientia,1970,26(2):214-216. 

[18］RAM H Y M,SETT R.Induction of fertile male flowers in genetically female Cannabis sativa,plants by silver nitrate and silver thiosulphate anionic complex[J].Theoretical and Applied Genetics,1982,62(4):369-375. 

[19］RAM H Y M,JAISWAL V S.Feminization of male flowers of Cannabis sativa L.by a morphactin[J].Die Natur-wissenschaften,1971,58(3):149-150.

[20］关凤芝.大麻遗传育种与栽培技术[M].哈尔滨：黑龙江人民出版社,2010.

[21］龚军辉.环境与性别分化[J].高等函授学报(自然科学版),2005,19(3):48-49.

[22］李广华.环境对于植物性别分化的影响[J].生物学杂志,2004,21(4):61.

[23］辛培尧,何承忠,孙正海,等.短日照处理对大麻开花及性别表达的影响[J].湖北农业科学,2008(7):776-778.

[24］WERF H M G,BERG W.Nitrogen fertilization and sex expression affect size variability of fibre hemp(Cannabis sativa L.)[J].Oecologia,1995,103(4):462-470.

[25］陈其本,余立惠,杨明,等.大麻栽培利用及发展对策[M].成都:电子科技大学出版社,1993.

[26］强晓霞.大麻性别分化的生理学研究[D].南京：南京农业大学,2012. 

[27］张玲玲.苦瓜性别分化的形态学及生理基础研究[D].南宁:广西大学,2006.

[28］汪俏梅,曾广文.苦瓜性别分化特异蛋白质的研究[J].植物学报,1998,40(3):241-246.

[29］陈璇,杨明,郭鸿彦,等.一种诱导纯雌性工业大麻的方法:CN104335895A[P].2015-02-11.

[30］董晓慧,景玉良,史冬梅,等.一种诱导纯雌性工业大麻的方法:CN111328711A[P].2020-06-26.

[31］李骏,刘祥忠,汤小苏.一种工业大麻植株的高效雌化方法：CN112655548A[P].2021-04-16.

[32］崔丹丹,杨瑞芳,佘玮,等.工业大麻性别鉴定研究进展[J].中国麻业科学,2018,40(5):244-248.

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备注/Memo

收稿日期：2021-09-08

基金项目：黑龙江省农业创新工程棚室种植技术（MLCX-16）；黑龙江省麻类（药用）体系组培与克隆协同创新岗（YYM19STX-19）。

第一作者：牛庭莉（1997-），女，硕士研究生，从事大麻单细胞培养研究。E-mail:1074349711@qq.com。

通信作者：戴凌燕（1977-），女，博士，教授，从事植物生理相关研究。E-mail:dailingyan770416@126.com。

更新日期/Last Update: 2022-04-01