HOU Ru-ni,KUANG Bing-hui,ZHANG Ling-mei,et al.Effect of Pleurotus ostreatus Scraps with Hyphal Activity on the Culturable Endophytes of Capsicum annuum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(12):68-73.[doi:DOI:10.11942/j.issn1002-2767.2021.12.0068]
未腐熟平菇菌糠对辣椒植株内可培养内生菌的影响
- Title:
- Effect of Pleurotus ostreatus Scraps with Hyphal Activity on the Culturable Endophytes of Capsicum annuum L.
- Keywords:
- Capsicum annuum L.; Pleurotus ostreatus scraps with hyphal activity; culturable microorganisms; endophytic bacteria; endophytic fungi
- 文献标志码:
- A
- 摘要:
- 为探究土壤中施用未腐熟平菇菌糠对栽培辣椒植株内生菌种类和数量的影响，用穴施不同量出菇两茬平菇菌糠处理土壤，以不加菌糠为对照，辣椒移植75 d时，采用常规组织分离法对辣椒植株茎和叶内的内生菌进行分离，200 d时，分离根内的内生菌，根据菌落形态初步区别种类，计数不同处理中各微生物的数量及种类。结果表明：不同处理分离出各内生菌的数量或种类均有不同，对照中分离出内生菌10种；167 g·穴-1处理分离的内生菌的种类最多，共11种，而其他处理均为9种。但从数量上来看，83 g·穴-1处理分离内生菌菌落数量最多，共285个，而125 g·穴-1处理分离的内生菌菌落数量最少，共105个。
- Abstract:
- In order to study the effect of the application of oyster mushroom waste with hyphal activity on the species and quantity of endophytes in pepper,the soil was treated with different amount of two-crops waste of Pleurotus ostreatus,and planted chilies in the soil.When the peppers were transplanted outdoors for 75 days,the endophytes were isolated from the stems and leaves of pepper plants by routine tissue isolation method,and the endophytesofits roots were isolated after transplanted for 200 days.The results showed that the number and species of endophytes were different in different treatments,10 species of endophytes were isolated from control,and the most endophytes were isolated by 167.0 g per hole treatment,there were 11 endophytes in total,and 9 in other treatments.However,in terms of quantity,the number of endophytes colonies was the highest in 83.0 g per hole treatment(285),but the number of endophytes colonies isolated from 125.0 g per hole was the least(105).
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备注/Memo
收稿日期：2021-08-14