SU Yu-jing,YAO Wen-min,CHEN Zhao-gui.Identify of Genetic Diversity in Phalaenopsis by ISSR Markers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(12):62-67.[doi:DOI:10.11942/j.issn1002-2767.2021.12.0062]
利用ISSR分子标记鉴定蝴蝶兰不同种资源的遗传多样性
- Title:
- Identify of Genetic Diversity in Phalaenopsis by ISSR Markers
- Keywords:
- Phalaenopsis; molecular markers; ISSR; genetic diversity
- 文献标志码:
- A
- 摘要:
- 为有效利用蝴蝶兰种质资源，促进蝴蝶兰新品种选育，本研究以不同的蝴蝶兰品种为研究对象,利用ISSR分子标记技术，通过DNA提取,优化ISSR-PCR扩增体系及聚类分析探究18种蝴蝶兰的遗传多样性。结果表明：8条ISSR引物经ISSR-PCR共扩增出61条条带，多态性条带为52条，平均多态性比率为86.0%。蝴蝶兰样品在遗传相似系数0.736水平上可分为三大类群，且第一大类群在0.760处可划分为两个类群。18种蝴蝶兰样品之间的相似系数为0.625 0~0.938 3，平均遗传相似系数为0.784 2，表明资源之间的遗传多样性适中，品种间差异不大。
- Abstract:
- In order to make effective use of Phalaenopsis germplasm resources and promote the breeding of new varieties of Phalaenopsis,this study investigated the genetic diversity of 18 varieties of Phalaenopsis by using ISSR molecular marker technology,extracting DNA,optimizing ISSR-PCR amplification system and clustering analysis.These results showed that 61 bands were amplified from 8 ISSR primers by ISSR-PCR,and 52 bands were polymorphic,with an average polymorphism ratio of 86.0%.The samples of Phalaenopsis were divided into three groups at the level of genetic similarity coefficient 0.736,and the first group could be divided into two groups at 0.760.The similarity coefficient between the 18 species of Phalaenopsis was 0.625 0-0.938 3,and the average genetic similarity coefficient was 0.784 2,indicating that the genetic diversity among the species was moderate,and there was little difference among the varieties.
参考文献/References:
［1］张果,赵玉安,刘晓鹏,等.国内蝴蝶兰育种技术的研究进展[J].贵州农业科学,2020,48(9):86-92.［2］ZIETKIEWICZ E,RAFALSKI A,LABUDA D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.［3］JUNG W S,CHUNG I M,KIM S H，et al.Direct shoot organogenesis from Lycium chinense Miller leaf explants and assessment of genetic stability using ISSR markers[J].Agronomy,2021,11(3):503.［4］IBRAHIM E A.Genetic diversity in Egyptian bottle gourd genotypes based on ISSR markers[J].Ecological Genetics and Genomics,2021,18:100079.［5］LIU J,LI X M,LIU Y Q,et al.Evaluation of genetic diversity and development of core collections of industrial brewing yeast using ISSR markers[J].Archives of Microbiology,2020,203(3):1-8.［6］段义忠,王海涛,张格格,等.基于ISSR分子标记的孑遗濒危植物四合木遗传结构分析[J].植物研究,2021,41（3）:466-473.［7］覃芳,史艳财,邹蓉,等.闹羊花ISSR-PCR反应体系建立及优化[J].北方园艺,2020(21):96-102.［8］孟珊,狄佳春,苏彩霞,等.江苏省扁豆地方种质资源遗传多样性评价[J/OL].植物遗传资源学报:1-18[2021-04-01].https://doi.org/10.13430/j.cnki.jpgr.20210302002.［9］陆婷,陈少瑜,吴涛,等.迪庆州核桃种质资源的遗传多样性与遗传结构[J].西部林业科学,2021,50(2):71-77.［10］吴国林.基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D].南昌：江西农业大学,2014.［11］孟丽娟,赵桂琴.红三叶ISSR-PCR反应体系的建立与优化[J].草原与草坪,2015,35(2):21-26.［12］黄亚芳.蒙顶甘露ISSR-DNA指纹图谱的初步构建[D].雅安：四川农业大学,2015.［13］荣广天.密花豆种质资源ISSR分析[D].长沙：中南林业科技大学,2016.［14］李建军,霍光,唐慧娟,等.60份红麻种质资源ISSR和RAPD的聚类分析[J].中国麻业科学,2015,37(6):273-279.［15］彭丽娟,牟柯澴,张健伟,等.基于SSR及ISSR标记的菜豆遗传多样性分析[J/OL].分子植物育种:1-15[2021-05-19].http://kns.cnki.net/kcms/detail/46.1068.S.20210318.1438.024.htm.［16］何荆洲,卢家仕,卜朝阳,等.36个蝴蝶兰品种遗传关系的ISSR分析[J].广东农业科学,2014,41(21):140-143.［17］谢启鑫,缪南生,宋小民,等.蝴蝶兰种质资源遗传多样性的ISSR分析[J].西北植物学报,2010,30(7):1331-1336.［18］赵谦.ISSR标记在植物遗传分析中的应用研究[D].汕头：汕头大学,2008.［19］谢琳丹.应用ISSR技术和观赏性状变异特性筛选蝴蝶兰杂交亲本[D].福州：福建农林大学,2014.［20］蹇黎.ISSR和SRAP标记技术在兰花植物种质资源研究中的应用[J].北方园艺,2011(23):190-192.［21］薄璇,侯艳霞,常宁,等.红枣分子标记技术应用研究进展[J].中国果菜,2020,40(9):56-60.［22］陈雅琦,苏楷淇,李春杰,等.醉马草DNA提取方法的对比与优化[J].草学,2020(5):32-38.［23］CHEN W H,JIANG Z Y,HSU H F,et al.Silencing of FOREVER YOUNG FLOWER-like genes from Phalaenopsis orchids promotes flower senescence and abscission[J].Plant and Cell Physiology,2021,62(1):111-124.［24］李佐,陈和明,肖文芳,等.蝴蝶兰GENOMIC-SSR与EST-SSR分子标记遗传差异性分析[C]//杜永臣.中国园艺学会2015年学术年会论文摘要集.北京:园艺学报编辑部,2015.［25］孙汝斌.分子标记技术在蝴蝶兰研究中的应用[J].品牌(下半月),2014(10):278.［26］崔学强,唐璇,黄昌艳,等.基于ISSR标记的卡特兰种质资源遗传多样性分析[J].西南农业学报,2020,33(7):1383-1388.［27］户帅雅,李斌奇,陈孝丑,等.红掌遗传多样性及亲缘关系的ISSR分析[J].福建农业学报,2020,35(1):20-27.
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备注/Memo
收稿日期：2021-09-09