BI Wei-wei,ZHAO Gui-xing,XIA Xiao-yu,et al.Effects of Light on Isoflavone and Protein During Germination of Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):37-41.[doi:10.11942/j.issn1002-2767.2020.12.0037]
光照对大豆萌发过程中蛋白质和异黄酮的影响
- Title:
- Effects of Light on Isoflavone and Protein During Germination of Soybean
- Keywords:
- light time; soybean isoflavones; different varieties; germination time; protein
- 文献标志码:
- A
- 摘要:
- 为提高大豆芽苗菜的品质,促进其工业化生产,以黑暗培养为对照,研究不同光照时间对12个大豆品种萌发后生长性状、蛋白含量及大豆异黄酮含量的影响。利用光照和黑暗处理比较分析不同品种大豆发芽过程中异黄酮的含量变化,利用分光光度法检测异黄酮的含量,利用近红外分析仪检测蛋白质含量。结果表明:光照对发芽率的影响不显著,光照有利于大豆芽长和产量的增加,光照增加了大豆芽蛋白质的含量。随着光照处理时间的延长,12个品种大豆芽中的异黄酮含量总体呈先增加后降低的趋势,与对照组相比,萌发第2天异黄酮含量增加较多的品种为黑农50、黑农48、黑农52、黑农63和黑农43,与籽粒相比,分别增加了174.33%、126.47%、107.05%、105.90%和104.89%,其中黑农52在萌发第2天时异黄酮含量达到最大,为10.87 mg?g-1。光照有利于大豆芽中异黄酮的积累,控制光照时间可以获得较多的异黄酮积累。
- Abstract:
- In order to improve the quality of soybean sprouts and promote its industrial production,this paper used dark culture as a control,studied the effects of different light time on the growth characteristics,protein content and soybean isoflavone content of 12 soybean varieties after germination.The light and dark treatments were used to compare and analyze the isoflavone content changes during the germination of different varieties of soybeans,the isoflavone content was detected by spectrophotometry,and the protein content was detected by the near-infrared analyzer.The results showed that the effect of light on germination rate was not significant.Light was beneficial to the growth of soybean sprouts length and yield.Light increased the protein content of soybean sprouts.With the extension of light treatment time,in general,the isoflavone content in 12 varieties of soybean sprouts first increased and then decreased.Compared with the control group,the varieties with more increased isoflavone content are Heinong 50,Heinong 48,Heinong 52,Heinong 63,and Heinong 43,increased by 174.33%,126.47%,107.05%,105.90% and 104.89%,respectively,of which Heinong 52 reached the maximum content of 10.87 mg?g-1 on the second day of germination.Illumination is conducive to the accumulation of isoflavones in soybean sprouts,and controlling the light time can obtain soybean sprouts with the most isoflavone accumulation.
参考文献/References:
[1]谢灵玲,赵武玲,沈黎明.光照对大豆叶片苯丙氨酸解氨酶(PAL)基因表达及异黄酮合成的调节[J].植物学通报,2000,17(5):443-449.
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备注/Memo
收稿日期:2020-07-31