LI Hui.Determination of Daidzein and Genistein in Sorbean by RP -HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2005,(05):6-8.[doi:10.11942/j.issn1002-2767.2005.05.0006]
大豆异黄酮检测技术的研究
- Title:
- Determination of Daidzein and Genistein in Sorbean by RP -HPLC
- 文章编号:
- 1002 -2767(2005)05 -0006 -03
- Keywords:
- soybean isoflavo ne ; HPLC ; daidzein ; geni stein
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在介绍了大豆异黄酮成分结构的基础上, 分析了几种主要测定方法的特点及应用范围, 并完善了HPLC 测定大豆异黄酮的方法。采用适宜的水解溶液和条件, 将大豆中的染料木甙和大豆黄酮甙分解为染料木素和大豆黄酮, 以染料木素和大豆黄酮为标准物质, 利用高效液相色谱定性定量检测水解液中的染料木素和大豆黄酮, 从而得出大豆异黄酮的含量。以2 mo l/L 的盐酸乙醇溶液为水解液, 水解时间2 h 。该方法快速、灵敏、重现性好, 回收率达96 %以上,RSD 小于3 %。
- Abstract:
- All kinds o f assay of soybean i sof lavone are repo rted and the assay condi tions were listed and compared in thi s paper .On the basi s of collecting and analyzing relating literature s at home and abroad , a metho d determination o f daidzein and genistein in soy bean by HPLC w as described .This method is rapid , sensi tive , reproduciable and provides a recovery of more than 96 % wi th RSD less than 3 %
参考文献/References:
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[ 7] Wang G.Ku an .Asimplif ied HPLC method for the det erminat ion of phyt oest rogens in soybean and it s p rocess ed products [ J] .Agric Food Chem , 1990 , 38 :185-190 .
[ 8] Adrian A .Fran k , Laurie J .cus ter , Camencit a M ., et al . .Qu an tit ati on of Phy toest rogens in legumes by HPLC[ J] .Agric Food chem ., 1994 , 42 :1905-1913 .
[ 9] 鞠兴荣, 袁建, 汪海波, 等.高效液相色谱法测定大豆提取物中大豆异黄酮的含量[ J] .中国粮油学报, 2000 , 15(4):26-29 .
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备注/Memo
收稿日期:2005 -03 -29
基金项目:黑龙江省农科院课题, 获黑龙江省农科院科技进步二等奖
作者简介:李辉(1975 - ), 女, 黑龙江省延寿县人, 助理研究员, 硕士, 主要从事谷物品质研究。