[1]金梦如,王帅,于春生,等.垦鉴稻10号遗传转化体系的建立[J].黑龙江农业科学,2020,(07):15-20.[doi:10.11942/j.issn1002-2767.2020.07.0015]
　JIN Meng-ru,WANG Shuai,YU Chun-sheng,et al.Establishment of Genetic Transformation System in Kenjiandao No.10[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(07):15-20.[doi:10.11942/j.issn1002-2767.2020.07.0015]
点击复制

垦鉴稻10号遗传转化体系的建立

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2020年07期 页码: 15-20 栏目: 出版日期: 2020-07-10
Title:
Establishment of Genetic Transformation System in Kenjiandao No.10
作者:
金梦如1; 王帅1; 于春生2; 孙冬梅1
1．黑龙江八一农垦大学 生命学院，黑龙江 大庆 163319；2.林口县农技推广中心，黑龙江 林口 157600
Author(s):
JIN Meng-ru1; WANG Shuai1; YU Chun-sheng2; SUN Dong-mei1
1.College of Life Sciences，Heilongjiang Bayi Agricultural University,Daqing 163319,China; 2.Linkou Agricultural Technology Extension Center,Linkou 157600,China
关键词:
水稻; 成熟胚; 愈伤组织; 转化
Keywords:
rice; mature embryo; callus; transformation
DOI:
10.11942/j.issn1002-2767.2020.07.0015
文献标志码:
A
摘要:
农杆菌介导的遗传转化技术,可优化水稻遗传转化体系。为研究农杆菌介导的遗传转化，以垦鉴稻10号去壳种子作为试验材料，研究了75%乙醇、0.1%升汞和5%次氯酸钠不同配比及处理时间消毒后的效果；利用试验筛选的最佳消毒条件，分别以MS培养基、B5培养基、N6培养基以及NMB培养基附加不同浓度2,4-D，筛选诱导愈伤的最佳培养基，并进行愈伤组织的转化。结果表明：诱导愈伤的最佳消毒处理组合为75%酒精1 min+0.1%升汞10 min+5%次氯酸钠4 min；经过方差分析NMB培养基附加2.5 mg?L-12,4-D对该品种愈伤组织的诱导效果明显优于其他培养基，诱导的愈伤组织大而洁白，硬度适中。将含p3300-ubi-BhGolS1-Tnos重组子的根癌农杆菌与愈伤组织共同培养，经检测，得到转化成功的愈伤组织。
Abstract:
Agrobacterium-mediated genetic transformation technology can optimize rice genetic transformation system.In order to study agrobacterium-mediated genetic transformation,taking Kenjiandao No.10 as the experimental materials,the effect of different matching and treatment time on the mature embryo callus were studied using 75% alcohol,0.1% mercuric chloride and 5% sodium hypochlorite as disinfectants.Use MS,B5,N6 and NMB as selective medium and add different concentrations of plant hormones to find out the best medium to inductive callus.The results showed that,the best callus induction disinfection treatment combination was:75% alcohol 1 min +0.1% mercuric chloride 10 min +5% sodium hypochlorite 4 min；after analysis of variance NMB medium with 2.5 mg?L-1 2,4-D was better than the other medium in callus induction,and the induced callus were larger with white color and moderate hardness.After cultivating Agrobacterium tumefaciens plasmid with vector p3300-ubi-BhGolS1-Tnos and callus together,the successful transformation of callus were obtained.

参考文献/References:

［1］张欣,付亚萍,周君莉,等.水稻规模化转基因技术体系构建与应用[J].中国农业科学,2014,47(21):4141-4154.

[2]张丽,宋玉霞,巩檑,等.农杆菌介导的马铃薯转化研究进展[J].分子植物育种,2015,13(7):1660-1667.
[3]苏振华,张俊华,张泽鑫,等.农杆菌介导的甜椒遗传转化体系的建立[J].江西农业学报,2019,31(6):9-15.
[4]高璐,薛永来.农杆菌介导的水稻快速高效基因转化系统[J].江苏农业科学,2019,47(4):39-42.
[5]朱克明,陶慧敏,徐硕,等.水稻愈伤分化和再生研究进展[J].种子,2016,35(11):55-59.
[6]王兴春,方红明,徐惠兰.提高粕稻优质米品种成熟胚培养力的研究[J].种子,2001,113(1):5-8.
[7]王萍,李杰,王恩旭,等.水稻成熟胚愈伤组织的诱导与增殖研究[J].种子,2008(5):17-19.
[8]刘香玲,王玉珍,罗景兰.水稻成熟胚愈伤组织的诱导和分化因素的研究[J].山东农业科学,2005,37(5):7-9.
[9]沈娟,徐利娟,张启军,等.不同接种方式对水稻成熟胚组织培养的影响[J].江苏农业科学,2012,40(8):67-69.
[10]郑责朝,胡事君.提高水稻愈伤组织植株再生能力几种方法的评价[J].杂交水稻,2005,20(2):54-57.
[11]Karthikeyan A,Pandian S T K,Ramesh M.High frequency plant regeneration from embryogenic callus of a popular indica rice(Oryza sativa L.)[J].Physiology and Molecular Biology of Plants,2009,15(4):371.
[12]朱克明,陶慧敏,徐硕,等.水稻愈伤分化和再生研究进展[J].种子,2016,35(11):55-59.
[13]Ge X,Chu Z,Lin Y,et al.A tissue culture system for different germplasms of indica rice[J].Plant Cell Reports,2006,25(5):392-402.
[14]廖鹏飞,赵君幸,雷发扬,等.不同基本培养基和激素对籼稻成熟胚愈伤组织诱导的影响[J].湖北农业科学,2012,51(18):4121-4125.

相似文献/References:

[1]迟莉.11%多·咪·福美双悬浮种衣剂防治水稻恶苗病田间药效试验[J].黑龙江农业科学,2013,(02):59.
　CHI LI.The Field Efficacy Trial of 11% Carbendazim?Prochloraz?Thiram FSC to Prevent Rice Bakanae Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):59.
[2]郭震华,刘传雪,张兰民,等.分子标记辅助选择在水稻抗稻瘟病育种中的研究进展[J].黑龙江农业科学,2013,(02):135.
　GUO Zhen-hua,LIU Chuan-xue,ZHANG Lan-min,et al.Research Progress of Molecular Marker-assisted Selection in Rice Blast Resistance Varieties Breeding[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):135.
[3]俄立生,刘婷婷,杜国春. 胜利农场2011~2012年水稻 展示品种对比与筛选[J].黑龙江农业科学,2013,(01):4.
　ELi-shegn,LIUTing-ting,DUGuo-chun.ContrastandSelectionofRiceDemonstrationVarietiesinVictoryFarmfrom2011to2012[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):4.
[4]王玉洋,蒋柳青,董雪梅,等. 不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(01):11.
　WANGYu-yang,JIANGLiu-qing,DONGXue-mei,et al.EffectofDifferentFactorsonRiceCallusFormation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):11.
[5]丁佳红,薛正莲,杨超英. 水杨酸对铜胁迫下水稻幼苗膜脂过氧化作用的影响[J].黑龙江农业科学,2013,(01):14.
　DINGJia-hong,XUEZheng-lian,YANGChao-ying.EffectofSalicylicAcidonMembraneLipidPeroxidationinRiceSeedlingsunderCopperStress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):14.
[6]王岩成,胡焕春,岳萍. 一定体积的水稻种子间隙吸水量的定量试验[J].黑龙江农业科学,2013,(01):27.
　WANGYan-cheng,HUHuan-chun,YUEPing.QuantitativeTestofRiceSeedsGapWaterAbsorptionofCertainVolume[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):27.
[7]解忠.不同温度对水稻灌浆期籽粒淀粉关键酶活性及稻米品质的影响[J].黑龙江农业科学,2014,(07):32.
　XIE Zhong.Effect of Different Temperature on Grain Starch Key Enzyme Activity and Rice Quality at Filling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):32.
[8]张智杰.牡丹江地区水稻生产现状及发展趋势[J].黑龙江农业科学,2014,(07):144.
　ZHANG Zhi-jie.Present Situation and Development Trends of Rice Production in Mudanjiang Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):144.
[9]王晓东,王茂青,王红霞.寒地水稻种质资源产量构成因素与产量的关系研究[J].黑龙江农业科学,2014,(06):5.
　WANG Xiao-dong,WANG Mao-qing,WANG Hong-xia.Analysis on the Relationship Between Yield Components and Yield of Rice Germplasm in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):5.
[10]张智杰.水稻粒形性状的QTL分析[J].黑龙江农业科学,2014,(06):15.
　LIN Hong,SUN Dequan,LI Suiyan,et al.Evaluation on Induction Rate of Maize Haploid Derived ?Lines for Different Groups F1[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):15.
[11]王瑞英.寒地粳稻成熟胚培养研究[J].黑龙江农业科学,2001,(05):19.[doi:10.11942/j.issn1002-2767.2001.05.0019]
　WANGRui-ying.Study on Culture Techniques of Mature Embryo of Cold Region Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2001,(07):19.[doi:10.11942/j.issn1002-2767.2001.05.0019]

备注/Memo

收稿日期：2020-01-12

基金项目：黑龙江农垦总局项目（HKKY190403）；大庆市指导项目（zd-2017-63）。
第一作者：金梦如（1995-），女，在读硕士，从事微生物学研究。E-mail:907401509@qq.com。
通信作者：孙冬梅（1970-），女，博士，教授，从事应用微生物研究。E-mail:sdmlzw@126.com。

更新日期/Last Update: 2020-08-21