WANGRui-ying.Study on Culture Techniques of Mature Embryo of Cold Region Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2001,(05):19-21.[doi:10.11942/j.issn1002-2767.2001.05.0019]
寒地粳稻成熟胚培养研究
- Title:
- Study on Culture Techniques of Mature Embryo of Cold Region Rice
- 文章编号:
- 1002 -2767(2001)05 -0019 -03
- Keywords:
- rice ; mature embryo ; differentiation ; variation
- 分类号:
- S511 .032
- 文献标志码:
- A
- 摘要:
- 试验表明, 寒地粳稻成熟种胚愈伤组织诱导率很高, 但分化率不够理想, 通过调整分化培养基中激素的浓度和配比来提高分化率, 收到了一定成效。诱导培养基用6 %浓度的蔗糖诱导出的愈伤组织分化率高于3 %和5 %两种浓度。R2 代存在大量可遗传变异, 通过田间细致观察比较和选择, 已获得一批优良变异株系, 正在进一步观察鉴定中。
- Abstract:
- Using nine varieties of japanica rice as materials , culture techniques of mature embryo of cold region rice are studied .The results show that the rate of induction is high , but the rate of differentiat ion is low .Dif ferentiation can be boosted through v arious regulator (IAA and KT)concent rations in differentiat ing medium .6 % sucrose is bet ter than 3 % and 5 % for dif ferentiation in callus format ion medium .There are lot s of variation that could be inheried in R2 .Some good materials are obtained throug h selection , w hich are in test and planting .
参考文献/References:
[ 1] 高振宇, 黄大年.影响籼稻幼胚愈伤组织形成和植株再生若干因素(简报)[ J] .植物生理学通讯, 1999 ,(4):113-115.
[ 2] 赵成章.提高水稻成熟种胚体细胞培养力的研究[ J] .中国水稻科学, 1987 , 1(3):171-176.
[ 3] 明凤, 李文雄, 胡尚连.小麦胚性愈伤组织发生影响因素的研究[ J] .东北农业大学学报, 2000 , 31(1):1-6.
[ 4] 朱秀英, 陈璋, 卢勤.水稻体细胞无性系的建立及其遗传变异的研究[ J] .遗传, 1990 , 12(6):1-4.
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备注/Memo
收稿日期:2001 -02 -21
作者简介:王瑞英(1972 -), 女, 黑龙江省虎林县人, 助研, 从事水稻生物技术育种研究