[1]郑明子,范志航,尹梦梦,等.高效促进番茄生长的生防木霉菌的筛选[J].黑龙江农业科学,2019,(01):48-54.[doi:10.11942/j.issn1002-2767.2019.01.0048]
　ZHENG Ming-zi,FAN Zhi-hang,YIN Meng-meng,et al.Screening of Trichoderma Strains with High-efficient Growth-promoting Activity on Tomato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(01):48-54.[doi:10.11942/j.issn1002-2767.2019.01.0048]
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高效促进番茄生长的生防木霉菌的筛选

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年01期 页码: 48-54 栏目: 出版日期: 2019-01-10
Title:
Screening of Trichoderma Strains with High-efficient Growth-promoting Activity on Tomato
作者:
郑明子1; 范志航1; 2; 3; 尹梦梦1; 2; 3; 郭坚华1; 2; 3; 蒋春号1; 2; 3
1.南京农业大学 植物保护学院，江苏 南京 210095；2.江苏省生物源农药工程中心，江苏 南京 210095；3.农业部作物病虫害监测与防控重点开放实验室，江苏 南京 210095
Author(s):
ZHENG Ming-zi1; FAN Zhi-hang1; 2; 3; YIN Meng-meng1; 2; 3; GUO Jian-hua1; 2; 3; JIANG Chun-hao1; 2; 3
1．College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China； 2．Engineering Center of Bioresource Pesticide in Jiangsu Province,Nanjing 210095,China； 3．Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing 210095,China
关键词:
木霉菌; 番茄; 促生; 生物防治; 酶活性
Keywords:
Trichoderma spp.;  tomato;  growth-promotion;  biological control;  enzyme activity
DOI:
10.11942/j.issn1002-2767.2019.01.0048
文献标志码:
A
摘要:
木霉既能够防治植物病害，还能促进植物生长，具有较高田间应用价值。为促进促生性木霉菌株的开发利用，以不同地区采集到的土壤和树皮样品中分离到的137株木霉菌株为参试菌株，利用平板对峙法初步筛选出对枯萎病菌、灰霉病菌拮抗效果较好的40株，根据菌株产滤纸酶活性、羧甲基纤维素酶活性、几丁质酶活性测定结果进一步复筛出20株木霉菌，用于温室试验效果评估。结果表明：在番茄上接种各木霉菌株的孢子悬浮液，接种后45 d测量、比较各菌株对番茄促生效果，最终筛选出的7株木霉菌可明显提高番茄株高、增加茎粗和叶片叶绿素含量，具有高效促进番茄生长作用，鉴定结果显示其中4株为哈茨木霉，2株为深绿木霉，1株为未知木霉属菌株。
Abstract:
The activities of Trichoderma spp.to control plant diseases and promote plant growth,which are with high application values in field.In order to promote the development and utilization of growth-promoting Trichoderma strains,137 Trichoderma strains were isolated from soil and bark samples from different areas,and 40 strains antagonistic against Fusarium Oxysporum and Botrytis cinerea were screened out from them.According to the abilities to secret cellulase,carboxymethyl cellulose enzyme and chitinase,20 strains were selected for green house experiment,in which tomatoes were inoculated with spore suspension of these 20 strains and efficiency of growth promoting were measured 45 days after treatments.The resuts showed that only 7 strains increased shoot length,stem width and chlorophyll concentration of tomatoes treated significantly,which were identified as 4 Trichoderma harzianum,2 Trichoderma atroviride,and 1 unknown Trichoderma strain.

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备注/Memo

收稿日期：2018-08-17基金项目：国家自然科学基金青年基金资助项目（31701829）；国家重点研发计划“新型高效生物杀菌剂研发”资助项目（2017YFD0201100）；黄淮海暖温带区设施蔬菜化肥农药减施技术模式建立与示范资助项目（2016YFD0201007）。第一作者简介：郑明子（1997-），女，在读学士，从事植物保护研究。E-mail：12115125@njau.edu.cn。通讯作者：蒋春号（1989-），男，博士，助理研究员，从事植物病虫害生物防治研究。 E-mail：chjiang@njau.edu.cn。

更新日期/Last Update: 2019-03-11