CUI Rui-feng,ZHANG Li-xia,HAN Xiao-hong.Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):8-9.
不同激素配比对百合鳞片愈伤组织诱导的影响
- Title:
- Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale
- 文章编号:
- 1002-2767(2014)09-0008-02
- Keywords:
- Lilium bulbiferum; scale; callus; induction
- 分类号:
- S682.29
- 文献标志码:
- A
- 摘要:
- 为筛选出适宜百合鳞片愈伤组织诱导的最佳激素配比,以给百合大规模的培养与繁殖创造有利的条件,以百合鳞片作为外植体,利用正交试验设计,研究不同激素配比对百合鳞片愈伤组织诱导的影响。结果表明:以MS为基本培养基,2,4-D浓度,6-BA浓度,NAA浓度3个因素对愈伤组织诱导率的影响表现为6-BA>NAA>2,4-D。最优的激素配比组合为A2 B1 C3,即2,4-D 0.6 mg.L-1,6-BA 0.3 mg.L-1,NAA 0.9 mg.L-1,其愈伤组织诱导率为91.21%。
- Abstract:
- For screening suitable optimized hormone combinations for inducing callus of lily scale,so as to create advantage conditions for culturing and propagating on a large scale,taking lily scales as explants,the effect of different hormone combinations on callus induction frequency of lily scales were studied with orthogonal design.The results showed that:taking MS as the basic culture medium,the effect of hormones on callus induction frequency was 6-BA>NAA>2,4-D.The optimized hormone combinations for inducing callus of lily scale was 2,4-D 0.6 mg.L-1 +6-BA0.3 mg.L-1+NAA 0.9 mg.L-1,the callus induction frequency was 91.21%.
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备注/Memo
收稿日期:2014-03-13
第一作者简介:崔瑞峰(1977-),女,山西省吕梁市人,硕士,讲师,从事植物生物技术相关教学与科研研究。E-mail:897824064@qq.com。