CHAI Wei-shu,TAN Xue-Ii,XIONG Li,et al.Hybrids Derived from Interspecific Hybridization in Lilium through Ovary Slice Technology[J].HEILONGJIANG AGRICULTURAL SCIENCES,2007,(03):67-69.[doi:10.11942/j.issn1002-2767.2007.03.0067]
子房切片技术获得百合杂交种
- Title:
- Hybrids Derived from Interspecific Hybridization in Lilium through Ovary Slice Technology
- 文章编号:
- 1002-2767(2007)03-0067-03
- Keywords:
- lilyi hybridization; embryo rescue; ovary slice
- 分类号:
- S681.9
- 文献标志码:
- A
- 摘要:
- 以不同杂种系的百合品种进行远缘杂交，采收授粉后5—20 d的子房，用子房切片培养方法挽救杂种胚，培育杂种。试验结果表明：百合远缘杂交中，采用子房切片方法挽救杂种胚，培育出杂种苗的关键是合适的胚龄：7—12 d的胚龄比较合适，这一胚龄段中，胚龄越小，胚萌发需要的时间越长。通过品种远缘杂交，培育出了少量的百合远缘杂种。
- Abstract:
- lnterspecific crosses were made to produce hybrids in Lilium, and the hybrid embryos were rescued by using the ovary slice culture after 5一20 days of pollination. The results showed that em- bryo age was a key factor to success in the ovary slice culture in Lilium interspecific hybridization. lf embryo age was younger, more days were required for embryo germination We got a few hybrids.
参考文献/References:
[1]陈俊愉．中国花经[M].上海；上海出版社，1998.180-183.
[2] Okazaki K,Asano Y,Oosawa K,et al. Interspecific Hybrid Be- tween Lilium"Oriental—Hybrid and Lilium"Asiatic”Hybrid with Revised Media[J]. Breeding Science,1994,( 44)159-64.
[3] Okazaki K. Application of Mutation Breeding Methods and O- rientation of Breeding in Ornamental Liliaceous[J]. Plant Hor- ticulture,2001,309-314,
[4] Van Tuyl J M. Overcoming Interspecifre Crossing Barriers in Lilium by Ovary and Embryo Culture[J]. Acta Hort,1990, 266.317-322.
[5] Fernandez A M, Nakazaki T, T8nis8ka T.Development of Diploid and Triploid Interspecific Hybrids between Lilium Lan- giflorum and L.Concolor by Orary Slice Culture[J]. Plant Breeding,1996,115: 167-171.
[6]沈革志，杨红娟，周根余，等．百合杂种胚的离体培养[J]．上海 农业学报，1997,13(1)；63-66.
[7]于晓英，吴铁明，倪沛，等．百合幼胚的离体培养和植株再生 [J]．湖南农业大学学报，2000,26(4):286-288.
[8] Van Tuyl.J M. Application of in vitro pollination,ovary culure and embryo rescue in breeding of Lilium Tulipa and Nerine[J]. Eucarpia,1990:86-97.
[9] Chi H. The Efficiencies of various embryo rescue methods in interspecific crosses of Lilium. Bot[J]. Bull Acad Sin, 2002, (43) .139-146.
[10]周厚高，张西丽，周焱．百合品种交配亲和性研究[刀．广西生物 科学．2000,19(4)1223-225.
[11]杨利平，张学方，高亦珂．10种百合属植物的传粉生物学[刀． 植物研究，1998,18(1)；63-38.
[12] Van Tuyl J lVL Application of in vitro pollination ovary culture 8nd embryo rescue for overcoming incongruity barriers in inter- speafic Lilium Crosses [J]. Plant Science, 1991,( 74): 115- 126.
[13] Roh M S,Robert J,Griesbach. Production and indentific8tion of interspecific hybrids of Lilium Longiflorum and Lilium ele- gans[J]. Acta Hort, 1996 ,414 ；93-96.
[14] Janson J,Michiel T.M Willense. Pollen tube penetration and fertilization jn Lilium Longiflorum(Liliaceae) [J]. American Journal of Botany,1995 ,82(2)1186-196.
[15] Sarmah B K, Sarla N. Hybrids between D.tenuisiliqua and B. campestris produced by embryo culture[J].J Gene and Plant breeding. 1998,58(1) ；35-39.
[16]壬蒂，冉毅东，戴朝曦，等．用胚挽救方法获得马铃薯种间杂种 植株[J].马铃薯杂志，1991,5(2)，79-82.
[17]赵淑清，郭剑波，常留印．特早熟杏胚培养试验[J].中国果树， 2001,(1) ；17-18.
相似文献/References:
[1]王郑昊,王传光,赵琦,等.多花兰与蕙兰杂交及种胚萌发研究[J].黑龙江农业科学,2013,(02):73.
WANG Zheng-hao,WANG Chuan-guang,ZHAO Qi,et al.Study on the Crossing between Cymbidium floribundumwith Cymbidium faberiduse and the Embryo Germination[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):73.
[2]丁海燕,张利敏,郑茂波,等.应用小麦-黑麦代换系间杂交培育小麦新品种探讨[J].黑龙江农业科学,2011,(04):4.
DING Hai-yan,ZHANG Li-min,ZHENG Mao-bo,et al.Study on Breeding High Yield Wheat by the Hybridization of Two Different Wheat-Rye Substitution Lines[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(03):4.
[3]杨佳明,赵兴华,屈连伟,等.喷施不同肥料对切花百合茎生根生长的影响[J].黑龙江农业科学,2013,(09):38.
YANG Jia-ming,ZHAO Xing-hua,QU Lian-wei,et al.Effects of Spraying Different Fertilizer on Rhizogenesis in Stem of Cut Lily[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):38.
[4]赵海滨.小麦与玉米杂交诱导矮败小麦产生单倍体的研究[J].黑龙江农业科学,2010,(05):19.
ZHAO Hai-bin.Studies on induct dwarfing-sterile wheat to create haploid by wheat×maize hybridization[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):19.
[5]韩冬伟.影响大豆杂交成活率因素分析[J].黑龙江农业科学,2010,(09):107.
HAN Dong-wei.Analysis of Factors Affecting the Survival Rate of Soybean Hybrid[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):107.
[6]李 双,袁 霖,贾桂霞.百合花粉管通道法遗传转化的研究[J].黑龙江农业科学,2011,(08):15.
LI Shuang,YUAN Lin,JIA Gui-xia.Study on the Pollen-tube Pathway Transformation in Lily[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(03):15.
[7]张锋,朱彦威,倪大鹏,等.腐殖酸肥对卷丹百合产量和品质的影响[J].黑龙江农业科学,2013,(11):34.
ZHANG Feng,ZHU Yan-wei,NI Da-peng,et al.Effects of Humic Acid Fertilizer on Yield and Quality of Lilium lancifolium Thunb[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):34.
[8]麻继仙,木万福,杨长楷,等.不同播期对萝卜杂交种种子质量和产量的影响[J].黑龙江农业科学,2013,(11):65.
MA Ji-xian,MU Wan-fu,YANG Chang-kai,et al.Effects of Different Sowing Dates on Yield and Quality of Radish Hybrid Seeds[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):65.
[9]崔瑞峰,张丽霞,韩晓红.不同激素配比对百合鳞片愈伤组织诱导的影响[J].黑龙江农业科学,2014,(09):8.
CUI Rui-feng,ZHANG Li-xia,HAN Xiao-hong.Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):8.
[10]李 璟,晏晓霞,蔡仕珍,等.三个百合品种光合特性研究[J].黑龙江农业科学,2015,(04):60.[doi:10.11942/j.issn1002-2767.2015.04.0060]
LI Jing,YAN Xiao-xia,CAI Shi-zhen,et al.Study on the Photosynthetic Characteristics of Three Lily Cultivars[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(03):60.[doi:10.11942/j.issn1002-2767.2015.04.0060]
备注/Memo
收稿日期：2006 -11-15
第一作者简介：柴卫淑（1979-），女，河南安阳人，硕士，讲师。从事花卉遗传改良研究。Tell 0533 - 6825003; E- mail: xuanca0000068@ sina. com.
通讯作者：谭学林．E-mail:rriyau@public. km. yn. en。