GAO Chong-jing,ZHANG Yan-bin,LI Ji-lin,et al.An A-PAGE Method Applied for Quality Breeding of Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):1-3.
适用于小麦品质育种的一种A-PAGE方法
- Title:
- An A-PAGE Method Applied for Quality Breeding of Wheat
- 文章编号:
- 1002-2767（2010）010-0001-03
- Keywords:
- wheat; quality; gliadin; near-isogenic lines(NILs); A-PAGE
- 分类号:
- S512
- 文献标志码:
- A
- 摘要:
- 为育种需要大批量地分析5+10亚基龙麦19中对小麦品质有较大影响的醇溶蛋白块品系，利用双板夹芯式垂直槽，凝胶面积为182 mm(宽)×95 mm(高)，分离胶浓度为6％，以甲酸为缓冲体系的不连续的酸性聚丙烯酰胺凝胶电泳（A-PAGE）来鉴定该醇溶蛋白块。结果表明：该系统可以较好地鉴定该醇溶蛋白块，具有简便、经济、高效、重复性好等特点，是适合于小麦育种过程中快速大量鉴定需要的一种A-PAGE电泳方法。
- Abstract:
- Due to breeding requirements,large quantities of gliadin block lines of Longmai 19 were needed to be identified.The gliadin block came from its donor cultivar Marquis and had great influence on the quality of wheat.In this study,a discontinuous acidic polyacrylamide gel electrophoresis(A-PAGE)was developed.Beijing 61 Factory DYCZ-30 dual vertical sandwich board slot was used because it could analyse 104 samples of wheat one time.The gel area was 182 mm(width)×95 mm(height).The buffer system was formic acid and the separating gel concentration was 6%.The results showed that this A-PAGE system could better identify some specific gliadin blocks.It was featured for simple,economical,efficient and well repeatable.It is suitable for rapidly identifying large numbers of gliadin blocks in the process of wheat breeding.
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备注/Memo
基金项目：国家自然科学基金资助项目（30871525）