[1]司　 亮.肾蕨离体培养技术的研究[J].黑龙江农业科学,2009,(05):20-22.[doi:10.11942/j.isn1002-2767.2009.05.0020]
　SI Liang.Studies on in vitro Culture of Nephrolepis auriculata[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(05):20-22.[doi:10.11942/j.isn1002-2767.2009.05.0020]
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肾蕨离体培养技术的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年05 页码: 20-22 栏目: 出版日期: 2009-09-10
Title:
Studies on in vitro Culture of Nephrolepis auriculata
文章编号:
1002-2767(2009)05-0020-03
作者:
司　 亮
(黑龙江省农业科学院园艺分院,黑龙江哈尔滨 150069)
Author(s):
SI Liang
(Horticultural Sub-academy of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang150069)
关键词:
肾蕨; 离体培养; 绿色小体(GGB)
Keywords:
Nephrolepisauriculata; in vitro culture; green globular bodies(GGB)
分类号:
S682
DOI:
10.11942/j.isn1002-2767.2009.05.0020
文献标志码:
A
摘要:
以肾蕨匍匐茎茎尖为外植体, 对离体再生培养技术进行了研究。 结果表明:GGB 的最佳诱导培养基为 MS+BA0.7 mg·L -1 , 诱导率为95%;GGB增殖培养基为 MS+BA0.7 mg·L -1 , 增殖系数为5.3;孢子体诱导培养基为MS+BA0.7 mg·L -1 +NAA0.7 mg·L -1 , 诱导率可达到 82%;适合诱导生根培养基为 MS+NAA0.2 mg·L -1 +0.1%Ac, 生根率为100%。
Abstract:
Using the stem apex of the stolon of Nephrolepisauriculata as explants, the study on in vito culture was conduc-ted.The results showed that the most appropriate induction culture medium of GGB was MS +BA0.7 mg·L -1 , the ratio ofinduction was 95%.The most proper multiplication medium was MS+BA0.7 mg·L -1 , multiplication coefficient was to be5.3;The medium for GGB to shoot was MS+BA0.7 mg·L -1 +NAA0.7 mg·L -1 , rate of inducement was 82%;The mostappropriate roots-planting culture medium was MS +NAA0.2 mg·L -1 +1.0% Ac, and the rooting rate wasup to 100%.

参考文献/References:

[ 1] 　吴北洪, 秦仁昌.中国蕨类植物科属志[ M] .北京:科学技术出版社,1991:4-7.

[ 2] 　邵莉楣.观赏蕨类的栽培与用途[ M] .北京:金盾出版社, 1994.
[ 3] 　林德钦, 张文珠, 郑国华.铁线蕨的离体快速繁殖[ J] .农业生物技术学报, 2007, 15(增刊):99-101.
[ 4] 　彭晓明,曾宋君.铁线蕨的组织培养及植株再生[ J] .植物生理学通讯,2004,40(5):575.
[ 5] 　曾宋君, 关丽.波士顿蕨的组织培养[ J] .植物生理学通讯, 1996, 32(3):198-200.
[ 6] 　程磊, 浦冰洁,周根余.若干理化因子对日本肾蕨绿色球状体分化及生长的影响[ J] .热带亚热带植物学报, 2001, 9(2):142-148.
[ 7] 　金建平, 兰涛,顾 等.皱叶肾蕨卷曲叶尖的离体培养[ J] .植物生理学通讯,1992(5):359-360

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备注/Memo

作者简介:司亮(1980-), 女, 山东维县人,硕士, 研究实习员, 主要从事组织 培养 和植 物基 因工 程研 究。 E-mail:siliang7698192 @163.com

更新日期/Last Update: 2016-11-27