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[1]谢立波,郭亚华,李景富,等.辣椒离体培养和植株再生体系建立[J].黑龙江农业科学,2006,(01):48-52.[doi:10.11942/j.issn1002-2767.2006.01.0048]
　XIE Li-bo,GUO Ya-hua,LI Jing-fu,et al.Establishment of in Vitro and Plant Regeneration System of Pepper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2006,(01):48-52.[doi:10.11942/j.issn1002-2767.2006.01.0048]

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辣椒离体培养和植株再生体系建立

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2006年01 页码: 48-52 栏目: 出版日期: 2006-01-10

Title:: Establishment of in Vitro and Plant Regeneration System of Pepper

文章编号:: 1002 - 2767(2006)01 - 0048 - 05

作者:: 谢立波¹; 郭亚华¹ ; 李景富² ; 王　雪¹ ; 邓立平¹; 1. 黑龙江省农科院园艺分院, 哈尔滨150069 ;
2. 东北农业大学, 哈尔滨150030

Author(s):: XIE Li-bo¹; GUO Ya-hua¹ ; LI Jing-fu² ; WANG Xue¹ ; DENG Li-ping¹; 1. Hort icultural Sub - academy of Heilo ng jiang Academy of Agricul tural Sciences , Harbin150069 ;
2. No rtheast Ag riculture University , Harbin 150086

关键词:: 辣椒; 离体培养; 植株再生

Keywords:: pepper ; tissue cul ture ; regenerat ion system

分类号:: S641. 304. 3

DOI:: 10.11942/j.issn1002-2767.2006.01.0048

文献标志码:: A

摘要:: 选3 种不同基因型的辣椒(Capsicum annuum L. )材料, 建立了辣椒高频离体再生体系, 试验结果表明:芽分化的最适培养基为:MS +6 - BA 5 mg /L +I AA 0. 2 mg /L ;芽伸长的最适培养基为MS +6 - BA 5 mg /L +I AA 0. 2 mg /L +GA3 2 mg /L ;生根的最适培养基为MS +NAA 0. 1mg /L , 当植株长到10 ～ 12 片叶时, 进行移栽。

Abstract:: Using the three dif ferent genotype s of the pepper as e xperimental materials. A rapid andef ficient in vit ro regeneration sy stem of pepper w as e stablished. The result s as fo llow s :the opt imumshoo t inducing medium is MS +6 - BA 5 mg /L +IAA 0. 2 mg /L , the optimum shoo t ex tentioninducing medium is MS +6 - BA 5 mg /L +I AA 0. 2 mg /L +GA3 2 mg /L , the optimum rootinducing medium is MS +NAA0. 1 mg /L. When the plant heig ht is 10 - 12cm , They can bet ransptanted.

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[ 5]王玉文, 杨美珠, 陈章良, 等. 甜椒的离体再生及基因转化[ J] .园艺学报, 1991, 33(10):780-786.

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[ 10]王纪方, 金波, 高秀云, 等. 蔬菜组织培养[ M] . 上海:上海科学技术出版社, 1983. 245-254.

[ 11]何晓明, 王鸣, 王喆之, 等. 辣椒子叶原生质体培养和植株再生[ J] . 园艺学报, 1997 , 24(3):298-300.

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备注/Memo

收稿日期:2005 - 04 - 03　

基金项目:黑龙江省“ 十五”科技攻关项目(GB02B101)　

第一作者简介:谢立波(1973 - ), 女, 黑龙江省绥化市人,

更新日期/Last Update: 2016-12-10