[1]杨贵春,刘海龙,王世发,等.大豆干旱和低温cDNA 文库的构建与检测[J].黑龙江农业科学,2009,(02):1-3.
　YANGGui-chun,LIU Hai-long,WANG Shi-fa,et al.Construction and Identification of cDNA Libraries of Soybean Treated byDrought and Low Temperature[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):1-3.
点击复制

大豆干旱和低温cDNA 文库的构建与检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年02 页码: 1-3 栏目: 出版日期: 2009-03-10
Title:
Construction and Identification of cDNA Libraries of Soybean Treated byDrought and Low Temperature
文章编号:
1002-2767(2009)02-0001-03
作者:
杨贵春1 ; 刘海龙1 ;  王世发1 ;  刘淑莲1 ;  韦正乙2 ; 蔡勤安2 ; 邢少辰2
1.吉林省农业科学院经济植物研究所, 吉林公主岭136105;2.吉林省农业科学院生物技术研究中心,吉林长春130124
Author(s):
YANGGui-chun1 ; LIU Hai-long1 ; WANG Shi-fa1 ; LIU Shu-lian1 ; WEI Zheng-yi2 ; CAI Qin-an2 ; XINGShao-chen2
1 .Indust rial Botany Institute of Jilin Academy of Agricultural Sciences ,Gongzhuling , Jilin 136105 ;2.BiotechnologyResearch Center of Jilin Academy of Agricultural Sciences ,Changchun , Jilin 130124
关键词:
大豆; 干旱; 低温; cDNA 文库; 酵母单杂交
Keywords:
soybean; droug ht ; low temperature ; cDNA library ; yeast one-hybrid
分类号:
S565.1
文献标志码:
A
摘要:
以栽培大豆(Glycine max)吉林35 为材料, 在幼苗期进行干旱、低温处理, 分离mRNA 经逆转录合成cDNA 后, 插入到融合表达载体中, 分别构建了干旱和低温两个融合表达文库。检测表明, 两个初始文库的滴度分别为2 .75×106pfu·mL-1和5.52×105 pfu·mL-1 ;插入的cDNA 片断长度在0.5～ 2.0 kb。此文库的构建为今后分离与干旱、低温相关基因奠定了基础。
Abstract:
Two kinds of mRNA were isolated from cultivated soybean variety Jilin 35 pre-treated with drought and low temperatureat seedling stage ,anti-transcripted into cDNA, to construct two relevant fusion expression libraries with insertion ofthese cDNA collection.The primary analy sis showed that the titer of two initial libraries were 2.75×106 pfu·mL-1(drought)and 5.52×105 pfu·mL-1(low temperature).The size of mostly insertion was between 0.5and 2.0 kb.The construction ofthese two libraries will be helpful for the related gene isolation in the future.

参考文献/References:

[ 1] 　雨水资源及其利用[ DB/ OL] .ht tp :// ww w .gnqx.gov.cn/ qxfw/jszy .asp.

[ 2] 　吴晓林, 琦祖.现代分子生物学实验技术[ M] .2 版.北京:中国协和医科大学出版社,1999 :319-328.
[ 3] 　李太武.中国对虾cDNA 文库的构建[ J] .动物学报, 1998 , 44(2):237 .
[ 4] 　LIU Qiang ,Kasuga Mie,Sakuma Yoh , et al.Tw o Transcripti on Factors, DREB1 and DREB2 , with an EREBP/ AP2 DNA Binding DomainSeparat e Two Cellular Signal Transduction Pathways inDrought- and Low-Temperature-Responsive Gene Expression, Respectively,in Arabidopsi s, The Plant [ J] .Cell , 1998 ,(10):1391-1406 .
[ 5] 　姚泉洪,邢彦彦, 王宗阳, 等.以酵母单杂交体系克隆水稻RAPB 基因cDNA 及其序列测定[ J] .中国科学(C 辑),1999 ,29(4):389-396 .
[ 6] 　谢永丽,王自章, 刘强,等.草坪草狗牙根中抗逆基因BeDREB 的克隆及功能鉴定[ J] .中国生物化学与分子生物学报, 2005, 21(4):521-527 .
[ 7] 　韩素英,张守攻, 汪泉,等.构建脱水反应元件(DRE)报告子用于小叶杨DRE 转录因子的克隆[ J] .农业生物技术学报, 2007 , 15(3):446-450 .
[ 8] 　QIN Feng, LI Jie, ZHANG Gui-you , et al.Isolation and St ructural Analysisof DRE-Binding Transcription Factor From Maize(zea maysL.)[ J] .Acta Botanaca Sini ca ,2003, 45(3):331-339 .

相似文献/References:

[1]吕静,韩莉梅,张所有.超氧化物歧化酶模拟物（SODM）对大豆保护酶系统的影响[J].黑龙江农业科学,2013,(01):23.
　LYUJing,HANLi-mei,ZHANGSuo-you.EffectsofSuperoxideDismutaseMimics(SODM)onSoybeanProtectiveEnzymeSystem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):23.
[2]吴俊彦,于晓光.黑河地区灾后补种大豆品种筛选试验[J].黑龙江农业科学,2014,(07):13.
　WU Jun yan,YU Xiao guang.Soybean Varieties Screening Test for Reseeding in Heihe Region after Disaster[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):13.
[3]安咏梅,王家军,李进荣,等.大豆抗胞囊线虫的分子标记研究[J].黑龙江农业科学,2014,(07):15.
　AN Yong mei,WANG Jia jun,LI Jin rong,et al.Molecular Marker Assisted Selection on Soybean Resistant to Soybean Cyst Nematode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):15.
[4]刘兴龙,王克勤,朱立新,等.大豆蚜对大豆阶段性危害研究[J].黑龙江农业科学,2014,(06):57.
　LIU Xinglong,WANG Keqin,ZHU Lixin,et al.Study on Effect of Soybean Aphid on Soybean Growth at Different Stages[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):57.
[5]赵 寅.富裕县大豆生产现状及发展建议[J].黑龙江农业科学,2014,(05):150.
　ZHAO Yin.Production Situation and Suggestions of Soybean in Fuyu County[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):150.
[6]郑 旭,赵秀梅,王连霞,等.25%阿维〖DK1〗·福〖DK1〗·莠锈悬浮种衣剂病害防效试验[J].黑龙江农业科学,2014,(05):54.
　ZHENG Xu,ZHAO Xiu-mei,WANG Lian-xia,et al.Field Efficacy Trial of 25% Abamectin·Thiram·Carboxin FSC Against Soybean Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):54.
[7]袁明. 齐齐哈尔大豆综合试验站现代农业产业示范基地建设成效的探讨[J].黑龙江农业科学,2013,(01):143.
　YUANMing.DiscussiononConstructionEffectofQiqiharSoybeanComprehensiveExperimentalStationofModernAgriculturalIndustrializationDemonstrationBase[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):143.
[8]李亮,李泽宇,王丽娜,等.不同大豆品种耐盐性表现分析[J].黑龙江农业科学,2013,(02):17.
　LI Liang,LI Ze-yu,WANG Li-na,et al.Performance Analysis on Salt Tolerance for Different Soybean Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):17.
[9]郑新利,张丙双,寇贺.东北地区大豆生产主要问题及发展对策[J].黑龙江农业科学,2012,(02):146.
　ZHENG Xin-li,ZHANG Bing-shuang,KOU He.Main Problems and Developing Countermeasuresof Soybean in Northeast China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(02):146.
[10]孙羽,冯延江,王麒,等.玉米茬原垄卡种大豆对其生长及产量的影响[J].黑龙江农业科学,2014,(03):26.
　SUN Yu,FENG Yan jiang,WANG Qi,et al.Effect of Original Ridge Tillage on the Growth and Yield of Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):26.
[11]张振宇.干旱条件下大豆叶片性状分析[J].黑龙江农业科学,2011,(11):18.
　ZHANG Zhen-yu.Soybean Leaf Traits under Drought Analysis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):18.
[12]赵桂范.干旱对不同大豆品种叶片的影响[J].黑龙江农业科学,2010,(10):19.
　ZHAO Gui-fan.Effect of Drought on Leaves of Different Soybean Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):19.
[13]赵桂范.干旱对合丰42 不同部位叶片发育的影响[J].黑龙江农业科学,2009,(01):29.
　ZHAO Gui-fan.Effect of Drought on Development of Different Part of Leaves of Hefeng 42[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):29.

备注/Memo

收稿日期:2008-11-04基金项目:国家植物转基因专项资助项目(J99-B-01)第一作者简介:杨贵春(1967-), 男, 吉林省公主岭市人, 硕士, 副研究员, 主要从事植物分子生物学和遗传育种研究。Tel :13843454181 ;E-mail:yanggch @126 .com 。通讯作者:邢少辰, E-mail:xingsc64 @yahoo.com .cn。

更新日期/Last Update: 2016-05-25