[1]唐凤兰,张晓东,王广金,等.利用花粉管通道法向小麦中导入麦谷蛋白高分子量优质亚基基因的研究初报[J].黑龙江农业科学,2002,(03):1-2.[doi:10.11942/j.issn1002-2767.2002.03.0001]
　TANG Feng-lan,ZHANG Xiao-dong,WANG Guang-jin,et al.The Preliminary Report on the Research of Introducing HMW and High Quality Gluten Subunit Gene into Wheat Through Pollen Tube Pathway[J].HEILONGJIANG AGRICULTURAL SCIENCES,2002,(03):1-2.[doi:10.11942/j.issn1002-2767.2002.03.0001]
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利用花粉管通道法向小麦中导入麦谷蛋白高分子量优质亚基基因的研究初报

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2002年03 页码: 1-2 栏目: 出版日期: 2002-05-10
Title:
The Preliminary Report on the Research of Introducing HMW and High Quality Gluten Subunit Gene into Wheat Through Pollen Tube Pathway
文章编号:
1002 -2767(2002)03 -0001 -02
作者:
唐凤兰1 ;  张晓东2 ;  王广金1 ;  李忠杰1 ;  张宏纪1 ;  孙　岩1
1 .黑龙江省农科院作物育种所, 哈尔滨150086 ;
2 .北京市农林科学院细胞工程实验室, 北京100081
Author(s):
TANG Feng-lan1 ;  ZHANG Xiao-dong2 ;  WANG Guang-jin1 ;  LI Zhong-jie1 ;  ZHANG Hong-ji1 ;  SUN Yan1
1 .Crop Breeding Institute , Heilongjiang Academy of Agricultural Sciences ,Harbin 150086 ;
2 .Beijing Ag ricultural Sciences, Beijing , 100081
关键词:
小麦; 花粉管通道; 基因导入; 高分子量麦谷蛋白亚基(HMW -GS)
Keywords:
w heat ; pollen tube pathway ; gene int roduction ; HMW -GS
分类号:
S512 .103.5
DOI:
10.11942/j.issn1002-2767.2002.03.0001
文献标志码:
A
摘要:
通过花粉管通道法向小麦中导入高分子麦谷蛋白(HMW -GS)优质亚基基因, 在D1 代用半粒法进行谷蛋白电泳检测, 获得3 粒种子含5 +10 亚基, 余下的半粒播入花盆中, 三株都已成活。后代变异很小, 除芒型变化外, 其他性状几乎没有变化, 这说明花粉管通道法导入HMW -GS 基因, 在改善小麦品质的同时, 能最大限度的保留受体优异的农艺性状。
Abstract:
HMW and hig h quality gluten subunit gene was int roduced into w heat through pollen tubepathw ay and determined w ith half -grain method by electrophoresis at the first generation .The resultshowed there w ere three g rains wi th 5 +10 gene .The other half -grain w as sow n into small -pot ,now these plants are all living .There w as no variatio n of agronomic t raits ex cept aw n ty pe .Thisshowed that w hen w heat quality w as improved by method of pollen tube pathw ay the parent’ s agronomict raits was retained .

参考文献/References:

[ 1] 赵友梅, 王淑俭.HM W 麦谷蛋白亚基的图谱在小麦品质研究中的应用[ J] .作物学报, 1990 , 16(3):208-218 .

[ 2] 傅宾孝, 赵友梅.小麦高分子量谷蛋白亚基与面粉品质[ J] .郑州粮食学院学报, 1989 ,(1):1-15 .
[ 3] 王金水, 赵友梅.小麦高分子麦谷蛋白与其加工品质的关系[ J] .郑州粮食学院学报, 1994 , 15(4):61-66 .
[ 4] Payne P.I .Genet ics of wheat storage prot eins and the ef fect of allelicvariation on b read making quali ty , Ann Rev[ J] .Plan t physiol.1987 , 38 :141-153 .
[ 5] 刘广田, 许明辉.普通小麦胚乳谷蛋白亚基的遗传研究, 高分子量谷蛋白亚基的多样性及其F1的遗传行为[ J] .中国农业科学,1988 , 21(1):56-60.

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备注/Memo

收稿日期:2002 -01 -03　

基金项目:黑龙江省自然科学基金资助项目部分内容。　
作者简介:唐凤兰(1963 -), 女, 辽宁省宽甸县人, 副研究员, 从事小麦育种及生物技术研究。

更新日期/Last Update: 2016-12-04