TANG Jingquan,LIU Wenlin,SUN Yan,et al.Molecular Detection and Resistance Analysis of the Stem Rust Resistance Gene Sr31 in Northeast Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(02):1-7.[doi:10.11942/j.issn1002-2767.2024.02.0001]
东北春小麦抗秆锈病基因Sr31的分子检测及其抗性分析
- Title:
- Molecular Detection and Resistance Analysis of the Stem Rust Resistance Gene Sr31 in Northeast Spring Wheat
- 文章编号:
- 1
- 关键词:
- 小麦; Sr31基因; 分子标记; 1BL/1RS易位系; 田间抗病性
- 文献标志码:
- A
- 摘要:
- 为促进东北春小麦抗性育种，利用与抗秆锈病基因Sr31连锁的分子标记SCSS30.2和iag95，对300份东北春小麦抗秆锈病基因进行分子检测及抗性分析。结果表明，在300份春小麦品种中，有8份小麦品种被检测到含有iag95或SCSS30.2分子标记，含有抗秆锈病Sr31基因，而小麦品种农林45号中只鉴定到了含有iag95标记，钢09-558中只鉴定到了含有SCSS30.2标记。通过ω-sec标记，发现9.67%的供试材料含有1BL/1RS易位系。其中，同时携带与抗性基因Sr31连锁的两个标记SCSS30.2和iag95的8份材料，均被检测到含有1BL/1RS易位系。田间抗性鉴定进一步表明，被SCSS30.2检测到的9个材料中，除黑春1号外，其余材料在田间均表现出了极强的抗病性。而仅被iag95标记检测到的农林45号，对21C3CTHQM和34MKGQM两类生理小种均表现为感病。本研究成功鉴定出携带Sr31基因及1BL/1RS易位系的小麦材料，可作为小麦抗病育种材料进一步利用。
- Abstract:
- In order to identify the presence of the resistance gene Sr31 in wheat materials the linked molecular markers SCSS30.2 and iag95 were used and to further determine the existence of the 1BL/1RS translocation in these materials. Using the PCR method, target fragments of 576 bp and 1100 bp were successfully amplified from positive control materials with SCSS30.2 and iag95, respectively. Among the 300 tested wheat materials, 10 varieties were detected with either the iag95 or SCSS30.2 molecular markers, with eight varieties carrying both markers. Using the ω-sec marker, we identified 9.67%, of materials possessing the 1BL/1RS translocation. Notably, all 8 materials identified as possibly containing the Sr31 gene were found to carry the 1BL/1RS translocation. Field disease resistance evaluations further revealed that, of the nine materials detected with the SCSS30.2 marker, all except Heichun 1 demonstrated strong field resistance. In contrast, the Nonglin 45, detected only with the iag95 marker, displayed susceptibility to both 21C3CTHQM and 34MKGQM pathotypes. In conclusion, this research successfully identified wheat materials carrying the Sr31 gene and the 1BL/1RS translocation, offering valuable molecular tools and germplasm resources for wheat resistance breeding.
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备注/Memo
收稿日期：2023-10-19