Naoganchaolu,ZHANG Sainan,ZHAO Mingmin,et al.Degradation Effects of Compound Strain 17N on Maize Stalks[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(12):82-86.[doi:10.11942/j.issn1002-2767.2023.12.0082]
复合菌系17N对玉米秸秆的降解作用
- Title:
- Degradation Effects of Compound Strain 17N on Maize Stalks
- 文章编号:
- 14
- Keywords:
- maize stalks; compound strain; degradation rate; degradation effect
- 文献标志码:
- A
- 摘要:
- 秸秆是一类重要的生物质能源，但由于秸秆直接还田后降解速率慢，影响下一茬作物的生长；因此，筛选秸秆降解菌对于农业生产中秸秆还田的高效利用尤为重要。为筛选能够快速高效降解玉米秸秆的菌系资源，将玉米秸秆壳分别浸泡于土浸液、土浸液(10%培养基)、土浸液(20%培养基)和纯净水中。结果表明，14和35 d时的土浸液对玉米秸秆壳的降解率均高于纯净水的降解率,且土浸液与土浸液(10%培养基)、土浸液(20%培养基)的降解率无明显差异。土浸液中进一步分离获得了复合降解菌系17N，用该复合菌系的发酵液浸泡三角瓶中的玉米秸秆壳，14和35 d时土浸液与复合菌系17N发酵液的降解率均高于纯净水。复合降解菌系17N发酵液对埋土玉米秸秆块具有良好的降解效果，优于三角瓶中对玉米秸秆块的降解效果。灭菌花盆土+灭菌网袋秸秆块+600 mL发酵液的降解率为37.0%，未灭菌花盆土+灭菌网袋秸秆块+600 mL发酵液的降解率为24.7%，三角瓶+发酵液+秸秆块的降解率为34.7%，不加发酵液的处理，即灭菌花盆土+灭菌网袋秸秆块处理的降解率仅为18.1%，明显低于3个加发酵液的处理。说明复合菌系17N对埋土玉米秸秆具有良好的降解作用。
- Abstract:
- Straw is a kind of important biomass energy. However， due to the slow degradation rate after the straw is directly returned to the field， it also affects the growth of the next crop. Therefore， screening degrading bacteria to straw is particularly important for the efficient utilization of straw in agricultural field.In order to screen bacterial resources that can degrade maize straw quickly and efficiently. In this study， maize straw husks were soaked in soil solution， soil solution (10% medium)， soil solution (20% medium) and purified water， respectively. The degradation rate analysis at 14 days and 35 days showed that the degradation rate of maize stalks husks by soil leaching solution was higher than that of pure water. There was no obvious difference in the degradation rate between soil solution and soil solution (10% medium) and soil solution (20% medium). The composite degrading strain 17N was isolated from soil solutions. When the fermentation broth of the composite bacterial strain was soaked in the maize stalks husks in the triangular flask， the degradation rate of the soil solution and the fermentation broth of the composite bacterial strain 17N was higher than that of pure water at 14 days and 35 days. The results showed that the composite degrading strain 17N fermentation broth had a good degradation effect on buried maize stalks blocks， superior to the degradation effect on maize stalks blocks in a triangular flask. The degradation rate of the treatment without fermentation broth， namely sterilized flower pot soil + sterilized mesh bag straw block， was obviously lower than that of the three treatments with fermentation broth. The degradation rate of sterilized flower pot soil + sterilized mesh bag straw block was 18.1%， the degradation rate of sterilized flower pot soil + sterilized mesh bag straw block + fermentation broth was 370%， the degradation rate of not sterilized flower pot soil + sterilized mesh bag straw block + fermentation broth was 24.7%， and the degradation rate of triangular flask + fermentation broth + straw block was 34.7%. The results indicated that the composite strain 17N had a good degradation effect on buried maize stalks.
参考文献/References:
［1］青格尔，于晓芳，高聚林，等.玉米秸秆低温降解复合菌系降解能力及微生物组成研究[J].中国生态农业学报，2020，28(11)：1753-1765.[2]MUZAMIL M，MANI I，KUMAR A，et al.An engineering intervention to prevent paddy straw burning through in situ microbial degradation[J].Journal of The Institution of Engineers (India)：Series A，2021，102(1)：11-17.[3]PETERSSON L，KVIEN I，OKSMAN K.Structure and thermal properties of poly (lactic acid) /cellulose whiskers nanocomposite materials[J].Composites Science and Technology，2007，67(11/12):2535-2544.[4]LOOSE J S M，FORSBERG Z,KRACHER D，et al.Activation of bacterial lytic polysaccharide monooxygenases with cellobiose dehydrogenase[J].Protein Science:a Publication of the Protein Society，2016，25(12):2175-2186.[5]BARSBERG S,SELIG M J,FELBY C.Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification[J].Biotechnology Letters，2013,35(2):189-195.[6]秦子鎛，刘子琪，曾庆亚，等.玉米秸秆还田对东北黑土地生态效益的影响[J].安徽农业科学，2012，40(18):9711-9714.[7]李泽义，高九思，张利敏，等.玉米秸秆还田应用效果研究[J].现代农业科技，2012(12):238-239.[8]FENG Y J，YU Y L，WANG X，et al.Degradation of raw corn stover powder (RCSP) by an enriched microbial consortium and its community structure[J].Bioresource Technology，2011，102(2)：742-747.[9]GAO Z M，XU X，〖KG(0.01mm〗RUAN L W.Enrichment and 〖KG)〗characterization of an anaerobic cellulolytic microbial consortium SQD-1.1 from mangrove soil[J].Applied Microbiology and Biotechnology，2014，98(1)：465-474.[10]周柳强，黄美福，罗文丽，等.粉碎和添加菌剂对红壤区自然堆沤条件下稻秆养分释放的影响[J].生态学报，2014，34(18)：5200-5205.[11]张建峰，侯红燕，付志金，等.速腐菌剂在东北地区秸秆堆肥中的功能验证及微生物菌群动态研究[J].中国农学通报，2013，29(26):112-117.[12]鲁耀雄，涂晓嵘，程新，等.稻草还田配施菌剂对晚稻生长和土壤酶活性的影响[J].中国土壤与肥料，2013(6):32-36.[13]BASSIL N M，SMALL J S，LLOYD J R.Enhanced microbial degradation of irradiated cellulose under hyperalkaline conditions[J].FEMS Microbiology Ecology，2020，96(7):fiaa102.[14]青格尔，高聚林，于晓芳，等.玉米秸秆低温高效降解复合菌系GF-20的温度和pH适应性研究[J].西北农林科技大学学报（自然科学版），2017，45(1):156-164.[15]王振，于晓芳，高聚林，等.玉米秸秆低温高效降解复合菌系GF-20的粉剂配方及其降解效果研究[J].中国农学通报，2020，36(21):69-74.[16]黄青盈，吕嘉昕，何秋愉，等.纤维素降解菌种的筛选测定及其对秸秆的降解[J].复旦学报(自然科学版)，2022，61(1):34-42.[17]王新光，田磊，王恩泽，等.玉米秸秆高效降解微生物复合菌系的构建及降解效果评价[J].生物技术通报，2022，38(4):217-229.[18]高娃，其布日，萨初拉，等.表达木质素降解酶的基因工程产朊假丝酵母菌部分生物学特性研究[J].中国饲料，2023(1):53-59.[19]白长胜，刘秋瑾，尹珺伊，等.产木质纤维素降解酶真菌的筛选及产酶特性[J].微生物学通报，2023，50(3):1098-1110.[20]袁宏，钱丹惠，叶文玲，等.一株促生秸秆降解菌的分离与鉴定［J］.微生物学杂志，2023,43(1):77-84.[21]台少华，扈进冬，位绍文，等.低温降解秸秆木霉菌的筛选、鉴定及功能评价[J].山东科学，2023，36(2):50-58.
相似文献/References:
[1]薛菁芳,陈书强,汪景宽.玉米秸秆对棕壤中可溶性无机氮和有机氮的影响[J].黑龙江农业科学,2011,(04):41.
XUE Jing-fang,CHEN Shu-qiang,WANG Jing-kuan.Effect of Maize Residues on Dissolved Organic Nitrogen andDissolved Inorganic Nitrogen in Brown Earth[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):41.
[2]王瑶,王宏燕,赵伟.微生物菌剂对玉米秸秆堆肥的作用效果研究[J].黑龙江农业科学,2013,(08):28.
WANG Yao,WANG Hong-yan,ZHAO Wei.The Effect Inoculating Microbes on Composting of Maize Stover[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(12):28.
[3]王珊珊,金昱言,赵凯.高效玉米秸秆降解复合菌系的筛选[J].黑龙江农业科学,2016,(04):39.[doi:10.11942/j.issn1002-2767.2016.04.0039]
WANG Shan-shan,JIN Yu-yan,ZHAO Kai.Screening of Composite Microbial System for Efficient Degradation of Maize Straw[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(12):39.[doi:10.11942/j.issn1002-2767.2016.04.0039]
[4]高亚冰,史风梅,裴占江,等.轻度炭化的玉米秸秆生物炭的燃烧行为研究[J].黑龙江农业科学,2016,(07):32.[doi:10.11942/j.issn1002-2767.2016.07.0032]
GAO Ya-bing,SHI Feng-mei,PEI Zhan-jiang,et al.Combustion Dynamic Analysis of Incompletely Carbonized Maize Stalk Biochar[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(12):32.[doi:10.11942/j.issn1002-2767.2016.07.0032]
[5]徐莹莹,刘玉涛,王宇先,等.玉米秸秆综合利用途径探讨[J].黑龙江农业科学,2016,(07):145.[doi:10.11942/j.issn1002-2767.2016.07.0145]
XU Ying-ying,LIU Yu-tao,WANG Yu-xian,et al.Introduction and Discussion on the Comprehensive Utilization of Maize Straw[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(12):145.[doi:10.11942/j.issn1002-2767.2016.07.0145]
[6]王美璇.吉林省玉米秸秆综合利用现状及建议[J].黑龙江农业科学,2017,(07):105.[doi:10.11942/j.issn1002-2767.2017.07.0105]
WANG Mei-xuan.Status and Suggestions of Maize Straw Comprehensive Utilization of Jilin Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(12):105.[doi:10.11942/j.issn1002-2767.2017.07.0105]
[7]郭伟,马传芳,马倩,等.秸秆配施氮素与腐植酸对玉米苗期系统抗性及土壤微生物的影响[J].黑龙江农业科学,2020,(04):32.[doi:10.11942/j.issn1002-2767.2020.04.0032]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):32.[doi:10.11942/j.issn1002-2767.2020.04.0032]
[8]孟祥海,李玉梅,邵广忠,等.玉米秸秆还田深度及腐熟菌对虫害的防控效果研究[J].黑龙江农业科学,2020,(06):59.[doi:10.11942/j.issn1002-2767.2020.06.0059]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):59.[doi:10.11942/j.issn1002-2767.2020.06.0059]
[9]王粟,李家磊,史风梅,等.玉米秸秆生物炭与牛粪混合厌氧发酵工艺优化[J].黑龙江农业科学,2020,(06):87.[doi:10.11942/j.issn1002-2767.2020.06.0087]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):87.[doi:10.11942/j.issn1002-2767.2020.06.0087]
[10]张楠,刘杰,于洪久,等.寒地玉米秸秆生物腐熟后断裂拉力值和减重值的变化[J].黑龙江农业科学,2020,(07):68.[doi:10.11942/j.issn1002-2767.2020.07.0068]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):68.[doi:10.11942/j.issn1002-2767.2020.07.0068]
备注/Memo
收稿日期：2023-08-10