[1]王一帆,王韫慧,李金红,等.大豆GmMS1雄性不育分子机制与功能分化研究[J].黑龙江农业科学,2022,(09):14-19.[doi:10.11942/j.issn1002-2767.2022.09.0014]
　WANG Yi-fan,WANG Yun-hui,LI Jin-hong,et al.Molecular Mechanism and Functional Differentiation of GmMS1 Male Sterility in Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(09):14-19.[doi:10.11942/j.issn1002-2767.2022.09.0014]
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大豆GmMS1雄性不育分子机制与功能分化研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年09 页码: 14-19 栏目: 出版日期: 2022-09-10
Title:
Molecular Mechanism and Functional Differentiation of GmMS1 Male Sterility in Soybean
作者:
王一帆; 王韫慧; 李金红; 蔺佳雨; 冯湘池; 曹振林; 姚士恩; 李美娜
广州大学 生命科学学院，广东 广州 510006
Author(s):
WANG Yi-fan;  WANG Yun-hui;  LI Jin-hong;  LIN Jia-yu;  FENG Xiang-chi;  CAO Zhen-lin;  YAO Shi-en;  LI Mei-na
School of Life Sciences， Guangzhou University， Guangzhou 510006， China
关键词:
大豆; 雄性不育; GmMS1; 驱动蛋白; 杂种优势
Keywords:
soybean;  male sterility; GmMS1; kinesin;  heterosis
DOI:
10.11942/j.issn1002-2767.2022.09.0014
文献标志码:
A
摘要:
为探究本实验室克隆的大豆ms1突变体位点编码基因（GmMS1）调控雄性育性的分子机制，通过原核表达分别获得GmMS1和其马达结构域单氨基酸置换突变体（GmMS1A263L）urms1的马达结构域（Motor Domain， MD）与His标签融合蛋白，GmMS1 MD-His和URMS1 MD-His蛋白。体外微管结合实验揭示urms1与微管结合能力显著降低，表明GmMS1第263位精氨酸是参与微管结合的关键氨基酸，因此urms1由于微管结合能力减弱从而影响其驱动蛋白功能，导致雄性不育；为解析GmMS1与其拟南芥同源基因AtNACK2（NPK1-activating kinesin）在进化上是否出现功能分化，利用CRISPR/Cas9技术创制AtNACK2马达结构域功能丧失型突变体，对育性表型进行观察，结果表明，AtNACK2功能丧失导致植株半不育，因此从遗传上证明GmMS1与AtNACK2的确出现了基因功能分化。
Abstract:
In order to explore the molecular mechanism of the locus coding gene (GmMS1) of soybean ms1 mutant cloned in our laboratory， we provided preliminary evidence in cell biology that GmMS1 may affect fertility by regulating the depolymerization of phragmoplast. To explore the molecular mechanism of GmMS1 in regulating male fertility， we produced the motor domain (MD) and His-tag fusion proteins of GmMS1 and its mutant urms1 with single amino acid replacement （GmMS1A263L）in motor domain， named GmMS1MD-His and URMS1MD-His， respectively. In vitro microtubule binding experiment revealed that the binding ability of urms1 to microtubules was significantly reduced， indicating that the arginine at position 263 of GmMS1 protein is the key amino acid involved in microtubule binding. Therefore， the male sterile phenotype of urms1 was assumed to weaken the microtubule binding ability. To compare whether GmMS1 and its Arabidopsis homologous gene AtNACK2 (NPK1 activating kinesin) had functional divergence during evolution， the AtNACK2 motor domain loss of function mutant was generated using CRISPR/cas9 technology. The male fertility results showed that the loss of function led to semi-sterile phenotype. Therefore， we first revealed that GmMS1 and AtNACK2 have gene functional divergence in controlling male fertility.

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备注/Memo

收稿日期：2022-06-02

基金项目：国家自然科学基金（32072084）。
第一作者：王一帆（1997－），女，硕士研究生，从事大豆杂种优势利用研究。E-mail:yifanwang0611@163.com。
通信作者：李美娜（1977－），女，博士，副教授，从事大豆杂种优势利用和生物钟与环境互作研究。E-mail:limeina@gzhu.edu.cn。

更新日期/Last Update: 2022-12-02