LI Yun-long.Isolation of the Endosymbiotic Actinobacteria PC-F1 and Identification Active Metabolites Against Pathogenic Fungi of Maize[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(10):64-67.[doi:10.11942/j.issn1002-2767.2016.10.0064]
抗玉米病原真菌的内生放线菌PC-F1的分离及活性物质鉴定
- Title:
- Isolation of the Endosymbiotic Actinobacteria PC-F1 and Identification Active Metabolites Against Pathogenic Fungi of Maize
- 文章编号:
- 1002-2767（2016）10-0064-04
- Keywords:
- actinomycetes; active metabolites; Bafilomycin B1
- 分类号:
- Q939.96
- 文献标志码:
- A
- 摘要:
- 放线菌是一类具有分支状菌丝形态的细胞，革兰氏呈阳性，与人类的生产、生活密切相关，被医药行业广泛应用的抗生素，其中70%都是从放线菌的代谢产物中获得。近年来，从新分离环境中获得稀有放线菌，并研究其活性代谢产物逐渐成为热点。本试验以从农业生境中采集的昆虫为研究对象，对其稀有放线菌进行筛选。结果表明：分离得到一株活性较好的内生放线菌PC-F1，并对其活性成分进行鉴定，初步判断是一种巴弗洛霉素系列化合物-巴弗洛霉素B1。
- Abstract:
- Actinomycetes are a class of branched hyphae cell morphology,Gram-positive.Closely related to the production of human life,antibiotics are widely used in the pharmaceutical industry,of which 70% were obtained from actinomycetes metabolites.In recent years,the new environment,access to scarce isolated actinomycetes,and to study its active metabolites become a hot spot.The test collected from agricultural habitats for the study of insects,rare screening of its actinomycetes,good activity was isolated strain within actinomycetes PC-F1,and identify its active ingredient,preliminary judgment was one kind Bafilomycin series compounds-Bafilomycin B1.
参考文献/References:
[1]
张玉便，张改云
.
南大西洋深海沉积物中可培养放线菌的多样性
[J].
应用海洋学学报，
2014
，
33(4): 509-515.
[2] 李春霞，苏俊.黑龙江省玉米主要病害的发生因素分析及其防治对策[J].黑龙江农业科学，2001(6): 38-39. [3] 魏刚.海洋放线菌Y12-26活性次级代谢产物的分离纯化、结构鉴定及其菌种鉴定 [D].上海：华东理工大学,2010. [4] Frndberg Emma，Petersson Carl，Lundgren L N，et al.Streptomyces halstedii K122 produces the antifungal compounds bafilomycin B1 and C1 [J].Canadian Journal of Microbiology,2000,46(8): 753-758. [5] Werner G，Hagenmaier H,Drautz H,et al.Metabolic products of microorganisms.224.Bafilomycins,a new group of macrolide antibiotics.Production,isolation,chemical structure and biological activity [J].Journal of Antibiotics,1984,37(2):110-117. [6] Bowman e J,Siebers A,Altendorf K.Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms,animal cells,and plant cells [J].Proceedings of the National Academy of Sciences of the United States of America,1988,85(21): 7972-7976. [7] Poulsen M,OH D-C,Clardy J,et al.Chemical Analyses of Wasp-Associated Streptomyces Bacteria Reveal a Prolific Potential for Natural Products Discovery [J].PLoS ONE,2011,6(2): e16763.
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备注/Memo
收稿日期：2016-09-21