[1]刘昭军,李　铁,刘　琦,等.大豆油份分子标记[J].黑龙江农业科学,2006,(06):6-8.[doi:10.11942/j.issn1002-2767.2006.06.0006]
　LIU Zhao-jun,LI Tie,LIU Qi,et al.RAPD Marker Related with Oil Content of Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2006,(06):6-8.[doi:10.11942/j.issn1002-2767.2006.06.0006]
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大豆油份分子标记

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2006年06 页码: 6-8 栏目: 出版日期: 2006-11-10
Title:
RAPD Marker Related with Oil Content of Soybean
文章编号:
1002 - 2767(2006)06 - 0006 - 03
作者:
刘昭军;  李　铁;  刘　琦;  刘丽艳;  李希臣
黑龙江省农科院生物技术研究中心, 哈尔滨150086
Author(s):
LIU Zhao-jun;  LI Tie ;  LIU Qi ;  LIU Li-yan ;  LI Xi-chen
Bio technolog y Research Center , Heilongjiang Academy o f Agricul tural Sciences , Harbin150086
关键词:
高油大豆; RAPD ; 分子标记
Keywords:
RAPD ; molecular marker ; oil content ; soy bean
分类号:
S565. 1
DOI:
10.11942/j.issn1002-2767.2006.06.0006
文献标志码:
A
摘要:
采用BSA 法对垦农18(高油, 23. 21 %)与黑农小粒豆(低油, 19. 0 %)的杂交F2 :3 群体进行RAPD 分析。用200 个随机引物对构建的高油DNA 池和低油DNA 池进行扩增, 有46 个引物产生了RAPD 扩增产物, 共获得315 个片断, 其中产生多态性片段的随机引物为4 个, 同高油含量相关的特征性条带分别为S56-900bp 、S61-600bp 、S107-600 /450 /370bp 、S134-450 /500bp 。这些标记平均可以区分F2 :3群体中87. 1 %的高油与低油单株, 重复性好, 可以用作高油含量的分子标记。
Abstract:
With Bulked Seg reg ation Analy sis , RAPD metho d w as employed to analy ze genome DNA of F2 :3 populat ion obtained by cro ssing Kennong 18(oil co ntent , 23. 21 %)w i th Heinongx iaolidou(oil content , 19. 0 %). Tw o hundred random primers w ere used to amplify betw een thehig h and low oil co ntent DNA poo ls , amo ng w hich there w ere 315 amplified f ragment s by 46primer s that produced RAPD product and w e found there w ere 4 random prime rs produced polymorphicbands. 4 markers rela ted w ith oil co ntent w ere S56-900bp 、S61-600bp 、S107-600 /450 /370bp 、S134-450 /500bp respectively. T hese markers could separate 87. 1 % high or low plant s inF2 :3 po pulatio n. The result s showed very stable and repeatable. The RAPD markers was useful to assistantthe selection fo r high oil content soybean plant s in soybean hybrid breeding.

参考文献/References:

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[ 2]关荣霞, 常汝镇, 邱丽娟, 等. 用于SSR 分析的大豆DNA 的快速提取[ J] . 大豆科学, 2003 , 22(1):73-74.
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备注/Memo

收稿日期:2006 - 08 - 24

基金项目:黑龙江省青年基金项目(QC04C34)
第一作者简介:刘昭军(1974 - ), 男, 黑龙江海林市人, 博士, 助研, 从事植物生物技术研究。E - mai l:liuz haoju n7 @y ah oo. com 。

更新日期/Last Update: 2016-12-14