[1]韩微波,唐凤兰,张月学,等.三叶草RAPD 反应条件的优选[J].黑龙江农业科学,2006,(01):10-12.[doi:10.11942/j.issn1002-2767.2006.01.0010]
　HAN Wei-bo,TANG Feng-lan,ZHANG Yue-xue,et al.Optimalization of RAPD Reaction System for Clover[J].HEILONGJIANG AGRICULTURAL SCIENCES,2006,(01):10-12.[doi:10.11942/j.issn1002-2767.2006.01.0010]
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三叶草RAPD 反应条件的优选

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2006年01 页码: 10-12 栏目: 出版日期: 2006-01-10
Title:
Optimalization of RAPD Reaction System for Clover
文章编号:
1002 - 2767(2006)01 - 0010 - 03
作者:
韩微波1; 唐凤兰1; 张月学1; 张弘强1; 姜艳喜1; 李道明1; 刘杰淋1; 蒿若超1; 白 晶2; 李集临2; 徐香玲2; 杨冬鹤2
1. 黑龙江省农科院作物育种所, 哈尔滨150086 ;
2. 哈尔滨师范大学生物系, 哈尔滨150080
Author(s):
HAN Wei-bo1 ;  TANG Feng-lan1 ;  ZHANG Yue-xue1 ;  ZHANG Hong-qiang1 ;  JIANG Yan-xi1 ; LI Dao-ming1 ;  LIU Jie-l in1 ;  HAO Ruo-chao1 ;  BAI Jing2 ;  LI Ji-l in2 ;  XU Xiang-l ing2 ;  YANG Dong-he2
1. Institute o f crop breeding , Hei long jiang A cademy of ag ricultural sciences , Harbin 150086 ;
2.Biolo gy depar tment , Harbin Normal U niversity , Harbin 150080
关键词:
苜蓿; RAPD ; 扩增反应; 优化
Keywords:
clover ; RAPD ; amplified reaction ; optimalization
分类号:
S541. 2
DOI:
10.11942/j.issn1002-2767.2006.01.0010
文献标志码:
A
摘要:
以红三叶( Tri f ol ium pretense L. )、白三叶(Tri f ol ium repens L . ) 和杂三叶(Tri f ol iumHybrid um L. )三种三叶草为材料, 对RAPD 反应条件中的dN TP 浓度、随机引物浓度、模板DNA用量、Taq DNA 聚合酶用量、退火温度以及预变性时间分别进行梯度试验。结果表明, 该反应体系体积为25 μL :ddH2O , 19. 1 μL ;10 ×Buf fer , 2. 5 μL ;Primer , 200 nM ;dNTP , 200 μM ;Taq DN A 聚合酶, 1U ;Template DNA 1ng /μL 。通过试验建立了一套稳定的RAPD 反应体系, 该反应体系扩增效果良好。
Abstract:
Three specie s of clover w ere used as the material s o f ex t ract ing DNA in this experiment, they w ere T rifolium pretense L. 、T rifolium repens L. and Trifolium Hybridum L. . Thee xperiment step to the concent rat ion of dN TP 、random primers 、template DNA 、Taq DNA polymerase、annealing temperature and preheat denaturalizatio n time on RA PD w ere conducted. Thena stable reactio n sy stem of RAPD w as set up. In the optimalization of RAPD sy stem wi th to talevolume o f 25μL :ddH2O , 19. 1μL ;10 ×Buf fer , 2. 5μL ;Primer , 200nM ;dN TP , 200μM ;Taq DNAE , 1U ;Template DNA 1ng /μL. The effect of amplifying w ith the reaction sy stem w as the mo stoptimal.

参考文献/References:

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备注/Memo

收稿日期:2005 - 10 - 19　

第一作者简介:韩微波(1979 -), 男, 陕西兴平人, 硕士, 实习研究员, 主要从事牧草诱发突变与生物技术育种研究。　
通讯作者:唐凤兰, 女, 研究员, E - mail :nkypzk @163. com

更新日期/Last Update: 2016-12-10